BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	541.2 / NA	158	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (77/81); total (77/81)

Only 100 of 158 total aligned reads displayed.

TCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAA > REL606/2193745‑2193812

tCGGAAATCAGCGCCAATAACAGCAAAACGCCAATcc                                 <  2:10496580/37‑1 (MQ=25)

tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcc                                 >  1:11093155/1‑37 (MQ=255)

tCGGAAATCAGCCCCAATAACAGCAAAACGACAATcc                                 >  2:2503087/1‑37 (MQ=38)

 cGGAAATCAGCCCCAATAACAGCAAAACGCCAATccc                                >  2:1945977/1‑37 (MQ=255)

 cGGAAATCAGCCCCAATAACAGCAAAACGCCAATccc                                <  1:1816644/37‑1 (MQ=255)

 cGGAAATCAGCCCCAATAACAGCAAAACGCCAATccc                                <  2:5843161/37‑1 (MQ=255)

  ggAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                               >  1:6274541/1‑37 (MQ=255)

  ggAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                               >  2:8901587/1‑37 (MQ=255)

  ggAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                               >  1:1516731/1‑37 (MQ=255)

   gAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                              <  2:10421582/37‑1 (MQ=255)

   gAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                              <  2:6482690/37‑1 (MQ=255)

   gAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                              >  2:570426/1‑37 (MQ=255)

   gAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                              >  2:3913740/1‑37 (MQ=255)

   gAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                              <  1:9188951/37‑1 (MQ=255)

   gAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                              >  2:10295610/1‑37 (MQ=255)

   gAAATCAGCCCAAACAACAGAAAAACGCCAATCCcca                              >  2:5800075/1‑37 (MQ=21)

    aaaTCAGCCCCAATAACAGCAAAACGCCAATCCccac                             <  1:10227588/37‑1 (MQ=255)

    aaaTCAGCCCCAATAACAGCAAAACGCCAATCCccac                             <  2:7089178/37‑1 (MQ=255)

    aaaTCAGCCCCAATAACAGCAAAACGCCAATCCccac                             <  1:5480619/37‑1 (MQ=255)

    aaaTCAGCCCCAATAACAGCAAAACGCCAATCCccac                             >  2:8104660/1‑37 (MQ=255)

    aaaTCAGCCCCAATAACAGCAAAACGCCAATCCccac                             >  2:9446189/1‑37 (MQ=255)

     aaTCAGCCCCAATAACAGCAAAACGCCAATCCccacc                            >  1:5208278/1‑37 (MQ=255)

     aaTCAGCCCCAATAACAGCAAAACGCCAATCCccacc                            >  2:2995919/1‑37 (MQ=255)

     aaTCAGCCCCAATAACAGCAAAACGCCAATCCccacc                            <  1:11028277/37‑1 (MQ=255)

     aaTCAGCCCCAATAACAGCAAAACGCCAATCCccacc                            <  2:2519550/37‑1 (MQ=255)

     aaTCAGCCCCAATAACAGCAAAACGCCAATCCccacc                            >  1:9012818/1‑37 (MQ=255)

      aTCAGCCCCAATAACAGCAAAACGCCAATCCccacca                           >  1:4559977/1‑37 (MQ=255)

      aTCACCCCCAATAACAGCAAAACGCCAATCCccacca                           <  1:10893254/37‑1 (MQ=25)

         aGCCCCAATAACAGCAAAACGCCAATCCTCACCAGAt                        >  1:8435560/1‑37 (MQ=25)

         aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                        >  2:4464647/1‑37 (MQ=255)

         aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                        >  2:10161855/1‑37 (MQ=255)

         aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                        <  1:3833301/37‑1 (MQ=255)

          gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                       >  1:4870305/1‑37 (MQ=255)

          gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                       <  2:9398155/37‑1 (MQ=255)

          gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                       <  1:3772891/37‑1 (MQ=255)

          gCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                       >  1:5793215/1‑37 (MQ=255)

           ccccAATAACAGCAAAACGCCAATCCCCACCAGATaa                      >  1:3989088/1‑37 (MQ=255)

           ccccAATAACAGCAAAACGCCAATCCCCACCAGATaa                      <  2:8549554/37‑1 (MQ=255)

            cccAATAACAGCAAAACGCCAATCCCCACCAGATaaa                     >  1:1718443/1‑37 (MQ=255)

             ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                    <  2:4784747/37‑1 (MQ=255)

             ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                    <  1:2432599/37‑1 (MQ=255)

             ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                    <  2:422758/37‑1 (MQ=255)

             ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                    >  1:7627781/1‑37 (MQ=255)

             ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                    <  2:698042/37‑1 (MQ=255)

              cAATAGCAGCAAAACGCCAGTCCCCACCAGATAAAGc                   <  2:8102060/37‑1 (MQ=37)

              cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                   >  2:6680762/1‑37 (MQ=255)

              cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                   >  2:10353706/1‑37 (MQ=255)

               aaTAACAGCAAGACGCCAATCCCCGCCAGATAAAGCa                  <  2:9406299/37‑1 (MQ=25)

               aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                  <  2:1680562/37‑1 (MQ=255)

               aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                  >  1:10661227/1‑37 (MQ=255)

                aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCAt                 <  2:2142834/37‑1 (MQ=255)

                 tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                <  2:9596014/37‑1 (MQ=255)

                 tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                >  1:5241136/1‑37 (MQ=255)

                  aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGa               <  2:820877/37‑1 (MQ=255)

                  aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGa               >  2:612962/1‑37 (MQ=255)

                  aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGa               <  1:9540889/37‑1 (MQ=255)

                  aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGa               >  1:7543441/1‑37 (MQ=255)

                  aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGa               <  1:9065281/37‑1 (MQ=255)

                   aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc              >  1:7501964/1‑37 (MQ=255)

                   aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc              <  1:3579288/37‑1 (MQ=255)

                   aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc              >  1:4969547/1‑37 (MQ=255)

                   aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc              >  2:3233584/1‑37 (MQ=255)

                   aCAGCAAAACACCAATCCCCACCAGATAAAGCATGAc              <  1:4201509/37‑1 (MQ=38)

                    cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg             >  2:10361976/1‑37 (MQ=255)

                    cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACg             <  1:908846/37‑1 (MQ=255)

                     aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGc            <  2:4211983/37‑1 (MQ=255)

                     aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGc            <  1:6597019/37‑1 (MQ=255)

                      gCAAAGCGCCAATCCCCACCAGATAAAGCATGACTcc           <  1:5382184/37‑1 (MQ=25)

                      gCAAAACGCCAATCCCCACCAGATAAAGCATGACGcc           <  1:7876770/37‑1 (MQ=255)

                       cAAAACGCCAATCCCCACCAGATAAGGCATGACGCCt          <  1:4585651/37‑1 (MQ=37)

                       cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt          <  2:7271386/37‑1 (MQ=255)

                       cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt          <  1:8631445/37‑1 (MQ=255)

                       cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt          <  1:8081571/37‑1 (MQ=255)

                       cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt          >  2:8682794/1‑37 (MQ=255)

                       cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt          <  1:7993965/37‑1 (MQ=255)

                       cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt          <  2:11235806/37‑1 (MQ=255)

                       cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt          >  1:1735236/1‑37 (MQ=255)

                       cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt          >  1:9686209/1‑37 (MQ=255)

                        aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTg         >  2:5090563/1‑37 (MQ=255)

                        aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTg         >  1:7373033/1‑37 (MQ=255)

                        aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTg         <  2:474262/37‑1 (MQ=255)

                        aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTg         >  2:1492927/1‑37 (MQ=255)

                        aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTg         <  2:2164214/37‑1 (MQ=255)

                        aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTg         <  2:396690/37‑1 (MQ=255)

                        aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTg         <  1:7797756/37‑1 (MQ=255)

                         aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGt        <  1:6791868/37‑1 (MQ=255)

                         aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGt        >  2:11282001/1‑37 (MQ=255)

                          aaCGCNAATCCCCACCAGATAAAGCATGACGCCTGTa       >  2:10616799/1‑37 (MQ=38)

                           aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt      >  1:7030624/1‑37 (MQ=255)

                           aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt      >  2:2929489/1‑37 (MQ=255)

                           aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt      >  2:825245/1‑37 (MQ=255)

                           aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt      <  1:10079293/37‑1 (MQ=255)

                            cGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt     >  1:381461/1‑37 (MQ=255)

                             gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa    <  1:8824197/37‑1 (MQ=255)

                             gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa    >  1:7319381/1‑37 (MQ=255)

                             gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa    >  1:3238778/1‑37 (MQ=255)

                             gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa    <  2:9820961/37‑1 (MQ=255)

                              ccAATCCCCACCAGATAAAGCATGACGCCTGTATTaa   >  1:8261190/1‑37 (MQ=255)

                               cAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa  <  1:5487483/37‑1 (MQ=255)

                               cAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa  >  1:3200210/1‑37 (MQ=255)

TCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAA > REL606/2193745‑2193812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`