Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,762,7410AT100.0% 447.5 / NA 124K662I (AAA→ATA) spoTbifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (78/46);  total (78/46)

Only 100 of 133 total aligned reads displayed.

ACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCT  >  REL606/3762707‑3762774
                                  |                                 
aCTTCGAATATTCAAAGTTTGAATACGGAAGAGATAg                                 >  2:1281721/1‑37 (MQ=255)
aCTTCGAATATTCAAAGTTTGAATACGGAAGAGATAg                                 >  2:3552365/1‑37 (MQ=255)
aCTTCGAATATTCAAAGTTTGAATACGGAAGAGATAg                                 >  2:9719058/1‑37 (MQ=255)
aCTTCGAATATTCAAAGTTTGAATACGGAAGAGATAg                                 >  2:8825398/1‑37 (MQ=255)
 cTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGa                                >  1:7427565/1‑37 (MQ=255)
 cTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGa                                <  2:8078721/37‑1 (MQ=255)
 cTTCGAATATTCAAAGTTTGAATACGGAAGAGATAGa                                <  1:7222499/37‑1 (MQ=255)
  ttCGAATATTCAAAGTTTGAATACGGAAGagatagat                               >  2:8715472/1‑37 (MQ=255)
  ttCGAATATTCAAAGTTTGAATACGGAAGagatagat                               >  1:3240353/1‑37 (MQ=255)
  ttCGAATATTCAAAGTTTGAATACGGAAGagatagat                               <  1:10280387/37‑1 (MQ=255)
  ttCGAATATTCAAAGTTTGAATACGGAAGagatagat                               >  2:7270509/1‑37 (MQ=255)
   tCGAATATTCAAAGTTTGAATACGGAAGAGATAGATg                              >  1:5947909/1‑37 (MQ=255)
   tCGAATATTCAAAGTTTGAATACGGAAGAGATAGATg                              >  1:8675952/1‑37 (MQ=255)
    cGAATATTCAAAGTTTGAATACGGAAGAGATAGATgg                             <  2:811415/37‑1 (MQ=255)
    cGAATATTCAAAGTTTGAATACGGAAGAGATAGATgg                             >  2:5418283/1‑37 (MQ=255)
    cGAATATTCAAAGTTTGAATACGGAAGAGATAGATgg                             <  2:8609225/37‑1 (MQ=255)
    cGAATATTCAAAGTTTGAATACGGAAGAGATAGATgg                             >  1:4128152/1‑37 (MQ=255)
     gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt                            >  1:2200022/1‑37 (MQ=255)
     gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt                            <  2:8571716/37‑1 (MQ=255)
     gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt                            <  1:3980088/37‑1 (MQ=255)
     gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt                            >  2:8891453/1‑37 (MQ=255)
     gAATATTCAAAGTTTGAATACGGAAGAGATAGATGGt                            >  2:6578214/1‑37 (MQ=255)
      aaTATTCAAAGTTTTAATACGGAAGAGATAGATGGTc                           <  2:7086882/37‑1 (MQ=37)
      aaTATTCAAAGTTTGAATACGGAAGAGATAGATGGTc                           <  2:482688/37‑1 (MQ=255)
      aaTATTCAAAGTTTGAATACGGAAGAGATAGATGGTc                           >  1:9553305/1‑37 (MQ=255)
      aaTATTCAAAGTTTGAATACGGAAGAGATAGATGGTc                           <  1:157033/37‑1 (MQ=255)
        tatTCAAAGTTTGAATACGGAAGAGATAGATGGTcgc                         >  1:10641232/1‑37 (MQ=255)
        tatTCAAAGTTTGAATACGGAAGAGATAGATGGTcgc                         >  2:2754126/1‑37 (MQ=255)
        tatTCAAAGTTTGAATACGGAAGAGATAGATGGTcgc                         <  2:7987599/37‑1 (MQ=255)
        tatTCAAAGTTTGAATACGGAAGAGATAGATGGTcgc                         <  2:6558970/37‑1 (MQ=255)
         atTCAAAGTTTGAATACGGAAGAGATAGATGGTcgcg                        >  2:10825613/1‑37 (MQ=255)
         atTCAAAGTTTGAATACGGAAGAGATAGATGGTcgcg                        >  1:11252403/1‑37 (MQ=255)
         atTCAAAGTTTGAATACGGAAGAGATAGATGGTcgcg                        >  1:8106456/1‑37 (MQ=255)
          ttCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGt                       <  2:11068011/37‑1 (MQ=255)
          ttCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGt                       <  2:8836089/37‑1 (MQ=255)
          ttCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGt                       <  1:6391853/37‑1 (MQ=255)
          ttCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGt                       >  1:11169378/1‑37 (MQ=255)
           tCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTc                      <  2:1573176/37‑1 (MQ=255)
           tCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTc                      >  1:8979074/1‑37 (MQ=255)
           tCAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTc                      >  1:4617961/1‑37 (MQ=255)
            cAAAGTTTGAATACGGAAGAGATAGATGGTCGCGTCt                     >  2:1291698/1‑37 (MQ=255)
             aaaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTa                    <  2:468902/37‑1 (MQ=255)
             aaaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTa                    <  1:4382037/37‑1 (MQ=255)
             aaaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTa                    >  1:9289147/1‑37 (MQ=255)
             aaaGTTTGAATACGGAAGAGATAGAGGGTCGCGTCTa                    <  2:6104706/37‑1 (MQ=37)
             aaaGTNTGAATACGGAAGAGATAGATGGTCGCGTCTa                    >  2:10601390/1‑37 (MQ=38)
              aaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTAc                   >  1:2633217/1‑37 (MQ=255)
              aaGTTTGAATACGGAAGAGATAGATGGTCGCGTCTAc                   >  1:7151794/1‑37 (MQ=255)
              aaGTTTGAATACGGAAGAGATAGATGGTCGCGTATAc                   >  1:1485521/1‑37 (MQ=37)
               aGTTTGCATCCGGAAGAGATAGATGGTCGCGTCTACa                  <  1:1935043/37‑1 (MQ=25)
               aGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACa                  <  1:11160321/37‑1 (MQ=255)
               aGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACa                  <  2:5269474/37‑1 (MQ=255)
               aGTTTGAATACGGAAGAGATAGATGGTCGCGTCTACa                  >  2:4087698/1‑37 (MQ=255)
                gTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAg                 >  1:6006012/1‑37 (MQ=255)
                gTTTGAATACGGAAGAGATAGATGGTCGCGTCTACAg                 >  2:3999915/1‑37 (MQ=255)
                 tttGAATACGGAAGAGATAGATGGTCGCGTCTACAgc                <  2:5526039/37‑1 (MQ=255)
                 tttGAATACGGAAGAGATAGATGGTCGCGTCTACAgc                >  2:9098768/1‑37 (MQ=255)
                 tttGAATACGGAAGAGATAGATGGTCGCGTCTACAgc                <  2:1522098/37‑1 (MQ=255)
                  ttGAATACGGAAGAGATAGATGGTCGCGTCTACAgcg               >  2:4504845/1‑37 (MQ=255)
                  ttGAATACGGAAGAGATAGATGGTCGCGTCTACAgcg               >  1:886265/1‑37 (MQ=255)
                  ttGAATACGGAAGAGATAGATGGTCGCGTCTACAgcg               >  1:4941727/1‑37 (MQ=255)
                   tGAATACGGAAGAGATAGATGGTCGCGTCTACAgcgc              <  2:7196530/37‑1 (MQ=255)
                    gAATACGGAAGAGATAGATGGTCGCGTCTACAGCGcc             <  1:923629/37‑1 (MQ=255)
                    gAATACGGAAGAGATAGATGGTCGCGTCTACAGCGcc             >  1:5131009/1‑37 (MQ=255)
                     aaTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCt            >  2:1718069/1‑37 (MQ=255)
                     aaTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCt            >  2:6693646/1‑37 (MQ=255)
                     aaTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCt            >  2:1453505/1‑37 (MQ=255)
                      aTACGGAAGAGATAGATTGTCGCGTCTACAGCGCCtt           <  1:8841280/37‑1 (MQ=37)
                      aTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCtt           >  1:2004797/1‑37 (MQ=255)
                      aTACGGAAGAGATAGATGGTCGCGTCTACAGCGCCtt           <  2:10730402/37‑1 (MQ=255)
                       tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt          >  1:1720800/1‑37 (MQ=255)
                       tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt          <  2:11109846/37‑1 (MQ=255)
                       tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt          >  2:4118809/1‑37 (MQ=255)
                       tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt          >  1:10996051/1‑37 (MQ=255)
                       tACGGAAGAGATAGATGGTCGCGTCTACAGCGCCttt          >  1:5856348/1‑37 (MQ=255)
                        aCGGAAGAGATAGATGGTCGCGTCTACAGCGCCtttc         >  1:3043561/1‑36 (MQ=38)
                        aCGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTa         <  2:7835761/37‑1 (MQ=255)
                        aCGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTa         >  1:5026920/1‑37 (MQ=255)
                        aCGGAAGAGATAGATGGTCGCGTCTACAGCGCCTTTa         >  1:6454680/1‑37 (MQ=255)
                         cGGAAGGGATAGATGGTCGCGTCTACAGCGCCTTTAt        <  1:7187405/37‑1 (MQ=38)
                         cGGAAGAGATAGGGGGTCGCGTCTACAGCGCCTTTAt        <  1:1419480/37‑1 (MQ=37)
                         cGGAAGAGATAGATGGTCGCGTCTACAGCGCCGTTAt        >  1:954427/1‑37 (MQ=38)
                          ggAAGAGATAGATGGTCGCGTCTACAGCGCCTTTAtt       >  1:9820470/1‑37 (MQ=255)
                          ggAAGAGATAGATGGTCGCGTCTACAGCGCCTTTAtt       >  1:6480295/1‑37 (MQ=255)
                          ggAAGAGATAGATGGTCGCGTCTACAGCGCCTTTAtt       >  1:7824961/1‑37 (MQ=255)
                           gAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTc      >  2:9858601/1‑37 (MQ=255)
                           gAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTc      >  1:9873076/1‑37 (MQ=255)
                           gAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTc      <  1:4473146/37‑1 (MQ=255)
                           gAAGAGATAGATGGTCGCGTCTACAGCGCCTTTATTc      >  1:2764566/1‑37 (MQ=255)
                           gAAGAGATAGATGGTCGCGTCTACAGCGACTTTATTc      >  2:6050411/1‑37 (MQ=37)
                            aaGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCg     >  2:808623/1‑37 (MQ=255)
                            aaGAGATAGATGGTCGCGTCTACAGCGCCTTTATTCg     <  2:1854107/37‑1 (MQ=255)
                             agagATAGATGGTCGCGTCTACAGCGCCTTTATTCGt    >  2:5046961/1‑37 (MQ=255)
                             agagATAGATGGTCGCGTCTACAGCGCCTTTATTCGt    >  2:10841508/1‑37 (MQ=255)
                             agagATAGATGGTCGCGTCTACAGCGCCTTTATTCGt    >  1:9493737/1‑37 (MQ=255)
                             agagATAGATGGTCGCGTCTACAGCGCCTTTATTCGt    <  1:3057188/37‑1 (MQ=255)
                              gagaTAGATGGTCGCGTCTACAGCGCCTTTATTCGTc   <  2:7359728/37‑1 (MQ=255)
                               agatagatGGTCGCGTCTACAGCGCCTTTATTCGTCt  >  2:5611678/1‑37 (MQ=255)
                               agatagatGGTCGCGTCTACAGCGCCTTTATTCGTCt  <  1:3999450/37‑1 (MQ=255)
                               agatagatGGTCGCGTCTACAGCGCCTTTATTCGTCt  <  1:10926553/37‑1 (MQ=255)
                                  |                                 
ACTTCGAATATTCAAAGTTTGAATACGGAAGAGAAAGATGGTCGCGTCTACAGCGCCTTTATTCGTCT  >  REL606/3762707‑3762774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑