| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,201,841 | Δ1 bp | coding (719/825 nt) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,201,839 | 0 | A | . | 96.2% | 116.1 / ‑1.3 | 26 | coding (721/825 nt) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (10/15); total (10/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01 | |||||||||||
TCCTTCGCCGCTTTAATCACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCA > REL606/4201766‑4201907 | tccttcGCCGCTTTAATCACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGaat < 1:927748/76‑2 (MQ=255) cttcGCCGCTTTAATCACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGaatag < 2:1147647/76‑4 (MQ=255) cgcTTTAATCACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTg < 2:379523/76‑1 (MQ=255) cgcTTGAATCACCATAGCGCCAAGCTCGGTCACGCTGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTg < 2:379506/76‑1 (MQ=255) cTTTAATCACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCg > 2:594673/1‑76 (MQ=255) tAATCACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCa < 1:234753/76‑1 (MQ=255) aaTCACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCaa < 1:726468/76‑1 (MQ=255) aTCACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCaaa < 2:622263/76‑1 (MQ=255) cACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACg < 1:471058/76‑1 (MQ=255) cgcCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACgg < 1:81369/76‑1 (MQ=255) cgcCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACgg > 1:681844/1‑76 (MQ=255) aaCTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTc < 1:31797/76‑1 (MQ=255) tCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCAt < 1:353197/76‑1 (MQ=255) cGGTCACGCGGTCATCGGNAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCg < 1:939810/76‑1 (MQ=255) ggTCACGCGGTCATCGGTAACACGTTAAATCGGT‑CGGGAATAGCAAGTGCGGCATACGGTTCACGGTGCTCATcta > 1:127474/1‑74 (MQ=255) cACGCGGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGaaaa < 2:389861/76‑1 (MQ=255) gcgGTCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATac > 2:111935/1‑76 (MQ=255) gcgGTCATCGGTAATACCTGCAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATac < 2:165970/76‑1 (MQ=255) tCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAATGGTTCACGCTGCTCATCGAAAATACACGc > 1:1077142/1‑76 (MQ=255) tCATCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGc > 1:803032/1‑76 (MQ=255) tCGGTAATACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCTCGGTGCTCATCGAAAATACACGCTGc < 1:111935/76‑1 (MQ=255) gTAATACGTGAAATCGGTCCAG‑AATCGAAATTGCGACAAACGGTTCCCGGTGCTCATCGAAAATACACACTGCAAg > 1:132947/1‑76 (MQ=255) aTACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGATAATACACGCTGCAAGGCa < 2:1080724/76‑1 (MQ=255) aTACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGTAAGGCa < 1:1116572/76‑1 (MQ=255) tACGTGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCaa > 2:525601/1‑76 (MQ=255) tGAAATCGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTa > 2:711082/1‑76 (MQ=255) aaaTCGGTCCGG‑AATAGAAAATGCGTCAAACGGTTAACGGTGCCCATCGAAAATACACGCTGCAAGGCTACGTacc > 1:127202/1‑74 (MQ=255) cGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCa > 1:735055/1‑76 (MQ=255) cGGTCCGG‑AATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCa < 1:293795/76‑1 (MQ=255) | TCCTTCGCCGCTTTAATCACCATTGCGCCAAACTCGGTCACGCGGTCATCGGTAATACGTGAAATCGGTCCGGAAATAGAAATTGCGGCAAACGGTTCACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCA > REL606/4201766‑4201907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |