| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 501,165 | (A)7→6 | intergenic (+98/+646) | ybbD → / ← ylbG | hypothetical protein/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 501,159 | 0 | A | . | 94.6% | 162.4 / 3.7 | 37 | intergenic (+92/+652) | ybbD/ylbG | hypothetical protein/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (17/18); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTG > REL606/501086‑501229 | ggTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGNATCAAAGAATGATCCAGATACaaa < 2:209202/76‑1 (MQ=255) tttAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaa < 2:403784/76‑1 (MQ=255) tttAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACaaaaa < 2:809370/76‑1 (MQ=255) ttGACAGTGAGGGCCAGGGATCAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTaa < 2:928528/76‑1 (MQ=255) ttGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTaa < 2:928524/76‑1 (MQ=255) tGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTaaa < 1:372174/76‑1 (MQ=255) aCAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAata > 1:656377/1‑76 (MQ=255) cAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAata < 2:691069/76‑1 (MQ=255) cAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAatat < 1:622520/76‑1 (MQ=255) aGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATg > 2:414870/1‑76 (MQ=255) aGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATg > 2:129287/1‑76 (MQ=255) tGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGt < 1:716883/76‑1 (MQ=255) gCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGAt > 2:777981/1‑76 (MQ=255) gggAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCa < 2:568558/76‑1 (MQ=255) cAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAATAAGTGTCGTGTAAATATGGTTAGATACCAGGGCa > 2:1064662/1‑76 (MQ=255) cAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGAGGTGTAAATATGTGAAGATACCAGGGCa > 2:535082/1‑76 (MQ=255) aGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAg < 1:486578/76‑1 (MQ=255) cGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTCAGATACCAGGGCAAGGGGAt > 2:999841/1‑76 (MQ=255) gAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATg < 2:575970/76‑1 (MQ=255) aaaaTCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATgg < 2:717075/76‑1 (MQ=255) aaaTCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGgt > 2:612844/1‑76 (MQ=255) aaaTCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGgt < 1:207064/76‑1 (MQ=255) aaaTCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATAgt < 1:207049/76‑1 (MQ=255) aTCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGgtgt < 2:713455/76‑1 (MQ=255) cGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTaa < 2:279736/76‑1 (MQ=255) tATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAAc < 2:1118701/76‑1 (MQ=255) tATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAAc < 2:1118695/76‑1 (MQ=255) aaaGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCttt > 2:888264/1‑76 (MQ=255) aaGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCtttt > 1:620066/1‑76 (MQ=255) gAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCtttttt < 1:671090/76‑1 (MQ=255) tGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTttg < 1:803891/76‑1 (MQ=255) gATCCAGATAC‑NAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTttgt > 1:169074/1‑76 (MQ=255) gATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAATGGGATGGTGTAAACCTTTTTTatgt > 2:1020068/1‑76 (MQ=255) aTCCAGATACAAAAAAAGTGTGGTATAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTttgt > 2:219358/1‑76 (MQ=255) aTCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTttgtt > 2:135000/1‑76 (MQ=255) aTCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTttgtt > 2:364041/1‑76 (MQ=255) tCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTttgttg > 2:428782/1‑76 (MQ=255) ccAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTgg < 2:556747/76‑1 (MQ=255) aGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAGACCTTTTTTTTGTTGGTg > 1:910370/1‑76 (MQ=255) aGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTg > 2:593930/1‑76 (MQ=255) | GGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTG > REL606/501086‑501229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |