| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,101 | +CA | coding (1205/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,099 | 1 | . | C | 91.7% | 127.5 / 6.2 | 36 | coding (1207/1677 nt) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (18/15); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01 | |||||||||||
| * | REL606 | 474,099 | 2 | . | A | 91.4% | 110.8 / 5.5 | 36 | coding (1207/1677 nt) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base A (18/14); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01 | |||||||||||
ACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTC‑‑CAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGG > REL606/474028‑474165 || aCCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTC‑‑Caca < 2:620675/76‑3 (MQ=255)aCCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTC‑‑Caca < 2:857190/76‑3 (MQ=255) accaGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTC‑‑CAGCGTCTGCTCtt < 1:179558/76‑1 (MQ=255) ccaGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGctct < 1:543768/76‑1 (MQ=255) naGTGGCGCATGGTTGCAAATNTCCACTNGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCtt < 1:1001738/75‑1 (MQ=255) gcgcATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGc < 2:292050/76‑1 (MQ=255) gcATGGTTGCAAATATCCGCTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGt < 1:914684/76‑1 (MQ=255) aTGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGttt < 2:906659/76‑1 (MQ=255) tGCAAATGTCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTc > 1:782230/1‑76 (MQ=255) tGCAAATATCCATTAGGAGCTGCACCTCTTCTTCAATCGCCTNATCGACTGCGTCTGNTCTTCCAGCGTTTCTGtn > 1:184977/1‑75 (MQ=255) gCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTcn > 1:1021722/1‑75 (MQ=255) aaTATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCg < 1:532477/76‑1 (MQ=255) atCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCa > 2:694816/1‑76 (MQ=255) ccACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGa < 2:460007/76‑1 (MQ=255) cACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGat > 1:528646/1‑76 (MQ=255) cACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGat > 1:62397/1‑76 (MQ=255) aCTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGata > 2:861416/1‑76 (MQ=255) ggATCTGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTtc > 2:849457/1‑76 (MQ=255) tCTGCTTCTCTTATTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTcc > 2:954192/1‑76 (MQ=255) cTGCTTCTCTTCTTCGATTGCCTCNTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCa > 2:198515/1‑76 (MQ=255) cTGCTTCTCATCTTCGATTGCCTCTTCTACAGCGTCTGATCTTCCAGAGTTACGGTATTCGCTAGANATTTCTCCa < 1:1086300/76‑1 (MQ=255) tGCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGATCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCag < 1:849457/76‑1 (MQ=255) gCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCagt < 1:141675/76‑1 (MQ=255) gCTTCTCTTCTTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCagt < 1:583244/76‑1 (MQ=255) ttctcttctTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCagtcg > 1:411897/1‑74 (MQ=255) ttctcttctTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCagtag > 1:411913/1‑76 (MQ=255) tctcttctTCGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCagtagt < 1:5857/76‑1 (MQ=255) tcGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCag < 2:290405/64‑1 (MQ=255) tcGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCag > 1:290405/1‑64 (MQ=255) tcGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAg < 1:626520/76‑1 (MQ=255) cGATTGCCTCTTCCGCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGt < 2:1157975/76‑1 (MQ=255) cGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTNGTGCGAACAGt < 1:170383/76‑1 (MQ=255) cGATTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGt > 1:427750/1‑76 (MQ=255) aTTGCCTCTTCCACAGCGTTTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTAc > 1:757186/1‑76 (MQ=255) aTTGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTAc > 1:329025/1‑76 (MQ=255) tGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCg > 1:41164/1‑76 (MQ=255) tGCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCg > 2:977834/1‑76 (MQ=255) gCCTCTTCCACAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCCGATATTTCTCCAGTAGTGCGAACAGNACCgg > 2:968362/1‑76 (MQ=255) || ACCGTAACCCACCAGTAGCGCATGGTTGCAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTC‑‑CAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGG > REL606/474028‑474165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |