| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,490,530 | (T)6→7 | intergenic (‑106/‑84) | glpE ← / → glpD | thiosulfate sulfurtransferase/sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,490,524 | 1 | . | T | 85.7% | 108.1 / 13.2 | 35 | intergenic (‑100/‑90) | glpE/glpD | thiosulfate sulfurtransferase/sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base T (15/15); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
GGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATG‑T‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAA > REL606/3490451‑3490593 | gggCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATG‑t‑t > 2:494392/1‑76 (MQ=255) tttACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATG‑t‑t‑tttttca > 2:865897/1‑73 (MQ=255) gCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTa < 2:867067/76‑1 (MQ=255) acaGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATG‑T‑T‑TTTTCAATGTTACCt > 1:808146/1‑76 (MQ=255) gTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTaa > 1:57915/1‑76 (MQ=255) gTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGCTACCTaa < 1:791280/76‑1 (MQ=255) gTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGcgcg > 1:440532/1‑76 (MQ=255) aaGCGGCAGATAAACGCCATAATGTTATACATATCACTCTCAAATGTT‑T‑TTTTGAATGTTACCTAAAGCGCGATTc > 1:864141/1‑76 (MQ=255) cGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCttt > 2:946613/1‑76 (MQ=255) cGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCttt < 1:647011/76‑1 (MQ=255) cAGATAAACGCCATAATGTTATACATATCACTCTAAAATG‑TCT‑TTTTNAATGTTNCCTAAAGCGCGATTCTCTGcn > 1:998708/1‑75 (MQ=255) aTAAACGCCATAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAat > 2:895531/1‑76 (MQ=255) tAAACGCCATAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAata < 2:666372/76‑1 (MQ=255) actccATAATGTTATACATATCACTCTTAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGt < 1:557256/73‑1 (MQ=255) ccATAATGTTATACATATCACGCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCg < 2:1005969/76‑1 (MQ=255) aTAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGAt < 1:214661/76‑1 (MQ=255) aTAATGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGAt > 2:1016246/1‑76 (MQ=255) tAATGTTATACATATCACTCTAAAATG‑T‑T‑TTTTCAATGTTACCGNAAGGGCGATTCTTTGCTAATATGTTCGATaa < 1:93720/76‑1 (MQ=255) aTGTTATACATATCACTCTAAAATGTT‑T‑TTTTCGATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAAc < 1:776102/76‑1 (MQ=255) aTGTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAAc < 2:817520/76‑1 (MQ=255) aTGTTATACATATCACTCTAAAATG‑T‑TCTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAAc < 1:494392/76‑1 (MQ=255) gTTATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGa > 2:571762/1‑76 (MQ=255) ttATACATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGaa > 1:878639/1‑76 (MQ=255) ataCATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATCACGAACa > 2:766560/1‑76 (MQ=255) taCATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACAt > 1:316704/1‑76 (MQ=255) aCATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACAtt < 2:72392/76‑1 (MQ=255) cATATCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACAttt > 2:9032/1‑76 (MQ=255) atatCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTa > 1:891421/1‑76 (MQ=255) tatCACTCTAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTAt > 1:790328/1‑76 (MQ=255) atCACTCTAAAATGTT‑T‑TTTTCAATGCTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATg < 1:569685/76‑1 (MQ=255) tctAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAGTATGTTCGATAACGAACATTTATGAGCtt < 1:1166825/76‑1 (MQ=255) ctAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCttt < 2:360688/76‑1 (MQ=255) ctAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCttt > 2:329197/1‑76 (MQ=255) ctAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCttt > 1:898504/1‑76 (MQ=255) tAAAATGTT‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTa < 1:1119040/76‑1 (MQ=255) tAAAATG‑T‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTaa > 2:500515/1‑76 (MQ=255) aaaaTGTT‑T‑TTTTCAATGTTACCTAAAGAGCGATTCTTTGCTAATATGTTCGATAACTAACATTTATGAGCTTTaa < 2:1081511/76‑1 (MQ=255) | GGGCAAATTTACCGCACAGTTTACGTCGAAGCGGCAGATAAACGCCATAATGTTATACATATCACTCTAAAATG‑T‑T‑TTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAA > REL606/3490451‑3490593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |