Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	73.6 / NA	28	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/18);  total (10/18)

TGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193711‑2193850                                                                     |                                                                        tGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc                                                                  <  2:293945/76‑1 (MQ=255) tGCATCCGGCGTCCAGGCGCGGCTGGACCGGCTGTCGGAAATCAGCCCCAATAACAGCAACAAGCCAATCCccacc                                                                  >  2:896599/1‑76 (MQ=255)     tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                              <  1:451546/76‑1 (MQ=255)         gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                          <  1:588507/76‑1 (MQ=255)                   gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                <  1:416074/76‑1 (MQ=255)                    cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCGACCAGATAAAGCATGACGCCTg                                               >  1:858834/1‑76 (MQ=255)                       cTGGTCTGGCTGTCGGATAGCAGCNCCAATAACAGCAAAACGCCAATCCCCACCAAATNAAGCATGACGCCTGTAt                                            <  2:991002/76‑1 (MQ=255)                       cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                            <  2:329911/76‑1 (MQ=255)                       cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                            <  2:221797/76‑1 (MQ=255)                        tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCACACCAGATAAAGCATGACGCCTGTAtt                                           >  1:707377/1‑76 (MQ=255)                         ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          >  2:601309/1‑76 (MQ=255)                         ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          <  2:168036/76‑1 (MQ=255)                         ggTCTGACTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          >  1:175179/1‑76 (MQ=255)                          gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                         <  1:35562/76‑1 (MQ=255)                           tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                        <  2:140909/76‑1 (MQ=255)                              ggCTGTCGGCAATCAGCNCCCATAACAGCAAAACGCCAATCCCCACCAGATNAAGCATCACCCCTGTATTATACAg                                     >  2:993881/1‑76 (MQ=255)                              ggCTGTCGGAAATCAGCCCCAATAACAGGAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     <  2:539782/76‑1 (MQ=255)                                cTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                   <  1:626582/76‑1 (MQ=255)                                      gAAATCAGCCCCAATAACNGCAAAACGCCAATCGCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                             <  1:939691/76‑1 (MQ=255)                                       aaaTCAGCCCCAATAACAGCGAAACGCCAATCCCCACCAGATAAAGCATGACTCCTGTATTAAACAGGCTTTTTAc                            <  1:896599/76‑1 (MQ=255)                                            aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                       <  2:114286/76‑1 (MQ=255)                                              ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                     >  2:938766/1‑76 (MQ=255)                                                     aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCATGTATTAAACAGGCTTTTTACCGATGGAGCATcgc              >  1:379218/1‑76 (MQ=255)                                                        aGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa           <  2:324767/76‑1 (MQ=255)                                                          cgaaaCGCCAATCCCCACCAGAAAAAGCACGACGCCTGTATTAAACAGGCTTCTTACCGATGGCGCATCTCGCAcc         >  1:1027701/3‑76 (MQ=255)                                                            aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg       <  2:812917/76‑1 (MQ=255)                                                            aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg       >  2:892738/1‑76 (MQ=255)                                                            aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg       <  2:403552/76‑1 (MQ=255)                                                               cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg    >  2:723547/1‑76 (MQ=255)                                                                 ccAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  <  2:868788/76‑1 (MQ=255)                                                                     |                                                                        TGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193711‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑