New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	293767 =	24 (1.000)	4 (0.180)	3/142	3.2	12.8%	intergenic (+37/‑490)	ykgD/ykgE	predicted DNA‑binding transcriptional regulator/predicted oxidoreductase
	?	REL606	293978 =	32 (1.420)					intergenic (+248/‑279)	ykgD/ykgE	predicted DNA‑binding transcriptional regulator/predicted oxidoreductase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGG  >  REL606/293698‑293841                                                                      |                                                                           tatCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCgg                                                                      >  1:683569/1‑76 (MQ=255)   tCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGa                                                                    <  2:594093/76‑1 (MQ=255)       gAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTtg                                                                <  2:221034/76‑1 (MQ=255)                   cAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCaa                                                    >  1:235835/1‑76 (MQ=255)                              ntGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAActatttctatt                                         >  1:726438/2‑76 (MQ=255)                              cTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAActatttctatt                                         <  2:668860/76‑1 (MQ=255)                                 aaTAAAACCGCGAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTTTGAGCCTTTCAACTATTTCTATTTcc                                      <  1:569350/76‑1 (MQ=255)                                 aaTAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTcc                                      <  2:373704/76‑1 (MQ=255)                                   tAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCaa                                    <  2:127224/76‑1 (MQ=255)                                     aaaCCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACg                                  <  2:325487/76‑1 (MQ=255)                                      aaCCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACgg                                 >  2:195402/1‑76 (MQ=255)                                      aaCCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACgg                                 >  1:61979/1‑76 (MQ=255)                                         cGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACggtgg                              >  1:8143/1‑76 (MQ=255)                                               aaaTCAGGGCAAAGATAATCCGCATTCCGGTAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTggg                        <  2:359016/76‑1 (MQ=255)                                               aaaTCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTggg                        >  1:50174/1‑76 (MQ=255)                                                  tCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCtt                     >  2:158306/1‑76 (MQ=255)                                                  tCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCtt                     <  2:493064/76‑1 (MQ=255)                                                   cAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCttt                    >  1:370322/1‑76 (MQ=255)                                                          aaGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATAtt             >  2:561580/1‑76 (MQ=255)                                                            gATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATAtttt           <  1:409951/76‑1 (MQ=255)                                                              tAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATAtttttt         >  2:304515/1‑76 (MQ=255)                                                               aaTCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTc        >  2:570557/1‑76 (MQ=255)                                                                   cttaTTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGAt    >  2:24822/4‑76 (MQ=255)                                                                   cttaTTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGAt    >  2:24821/4‑76 (MQ=255)                                                                    gCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATg   >  2:215336/1‑76 (MQ=255)                                                                     cATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATgg  >  1:226440/1‑76 (MQ=255)                                                                      |                                                                           TATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGG  >  REL606/293698‑293841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.