| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,928,884 | T→G | V223G (GTA→GGA) | rho → | transcription termination factor Rho |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,928,884 | 0 | T | G | 100.0% | 44.3 / NA | 12 | V223G (GTA→GGA) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (5/7); total (5/7) | |||||||||||
GGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGAT > REL606/3928816‑3928956 | ggATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGGAAAAGGt < 1:736981/76‑1 (MQ=255) tGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGGAAAAGGTGAAGTTGTTGc > 2:196241/1‑76 (MQ=255) aTGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGGAAAAGGTGAAGTTGTTGCtt < 1:499072/76‑1 (MQ=255) ctgATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGGAAAAGGTGAAGTTGTTGCTTCTACCTTTg > 2:664878/1‑76 (MQ=255) cgacgaACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGGAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGaa < 1:296177/76‑1 (MQ=255) aaCGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGGAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGc < 2:450925/76‑1 (MQ=255) gaagaaGTAACCGAGATGCAGCGTCTGGGAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGcc > 1:545186/1‑76 (MQ=255) aagTAACCGAGATGCAGCGTCTGGGAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGt < 2:31774/76‑1 (MQ=255) aaCCGAGATGCAGCGTCTGGGAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAg > 2:121531/1‑76 (MQ=255) gaTGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCg < 2:339666/76‑1 (MQ=255) gCAGCGTCTGGGAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGaa < 1:144054/76‑1 (MQ=255) tCTGGGAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTg < 2:467944/76‑1 (MQ=255) tGGGAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGAt > 2:173437/1‑76 (MQ=255) | GGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGAT > REL606/3928816‑3928956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |