Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,168,340	A→C	E1127A (GAA→GCA)	rpoC →	DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,168,340	0	A	C	100.0%	86.8 / NA	22	E1127A (GAA→GCA)	rpoC	DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/13);  total (9/13)

TAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGC  >  REL606/4168269‑4168400                                                                        |                                                             tAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATcc                                                          >  2:127760/1‑76 (MQ=255)  aaaGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCcg                                                         >  2:475936/1‑76 (MQ=255)        aTTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTa                                                   <  2:672230/76‑1 (MQ=255)          tGTTCAGCTGGAAGATGGCGTACAGATCAACTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTAcc                                                 <  1:146912/76‑1 (MQ=255)           gTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCa                                                >  2:545009/1‑76 (MQ=255)            ttCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCaa                                               <  1:44384/76‑1 (MQ=255)            ttCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCaa                                               <  2:353795/76‑1 (MQ=255)                gCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGTGTATTCCGCAGGCATCCGGCGGTACCAAGGAc                                           <  1:317045/76‑1 (MQ=255)                    gAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCa                                       <  1:508240/76‑1 (MQ=255)                         tGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCggt                                  <  2:669181/76‑1 (MQ=255)                          ggCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCggtg                                 >  1:685159/1‑76 (MQ=255)                          ggCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCggtg                                 >  2:446501/1‑76 (MQ=255)                              tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCt                             <  1:230411/76‑1 (MQ=255)                                        ctctGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGtt                   <  2:746172/76‑1 (MQ=255)                                           tGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCg                <  2:121618/76‑1 (MQ=255)                                              tGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGAc             >  2:667955/1‑76 (MQ=255)                                                acacCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGGTGCGGACCt           >  2:67357/1‑76 (MQ=255)                                                 cacCCTGGCGCGTATTCCGCAGGCATCCGTCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTg          <  2:231630/76‑1 (MQ=255)                                                   cccTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGtt        <  2:351350/76‑1 (MQ=255)                                                    ccTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGAGGACCTGTTc       >  2:160817/1‑76 (MQ=255)                                                     cTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGTGGACCTGTTCg      <  2:448278/76‑1 (MQ=255)                                                         cgcgTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGc  >  1:376207/1‑76 (MQ=255)                                                                        |                                                             TAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGC  >  REL606/4168269‑4168400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑