Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,327,115	A→G	I252T (ATT→ACT)	mreC ←	cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,327,115	0	A	G	100.0%	96.4 / NA	24	I252T (ATT→ACT)	mreC	cell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/17);  total (7/17)

CCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAA  >  REL606/3327043‑3327185                                                                         |                                                                       ccGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTca                                                                     <  1:579588/76‑1 (MQ=255)        gATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGtata                                                              <  2:166402/76‑1 (MQ=255)           cTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAgc                                                           >  2:597656/1‑76 (MQ=255)              ccccccACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAgcgcg                                                        <  2:94143/76‑1 (MQ=255)                  ccACAGCAGCAGCAGATAACCCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTgg                                                    >  1:10543/1‑76 (MQ=255)                    acaGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGt                                                  <  2:486343/76‑1 (MQ=255)                        cagcagcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCg                                              <  1:139912/76‑1 (MQ=255)                        cagcagcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCg                                              >  1:192362/1‑76 (MQ=255)                             gcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGttt                                         <  2:403301/76‑1 (MQ=255)                              cagATAACGCAAGCGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGtttt                                        <  2:555768/76‑1 (MQ=255)                               agATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTa                                       <  1:740931/76‑1 (MQ=255)                               agATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTa                                       <  1:339395/76‑1 (MQ=255)                               agATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTa                                       <  2:309568/76‑1 (MQ=255)                                       cAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAagag                               <  2:570202/76‑1 (MQ=255)                                          aCGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACa                            <  1:275364/76‑1 (MQ=255)                                           cGTTGCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACaa                           <  1:525363/76‑1 (MQ=255)                                               gCAGCCCTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACcgc                       >  1:556337/1‑76 (MQ=255)                                                     cTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCgg                 >  2:369603/1‑76 (MQ=255)                                                     cTGCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCgg                 >  1:173670/1‑76 (MQ=255)                                                       gCAGTCGGACGCGCCTGAGTCACAGTATAATCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGAt               <  2:47806/76‑1 (MQ=255)                                                       gCAGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGAt               <  1:694716/76‑1 (MQ=255)                                                         aGTCGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAg             >  2:366836/1‑76 (MQ=255)                                                            cGGACGCGCCTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGcca          <  2:78033/76‑2 (MQ=255)                                                                    ccTGAGTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGaa  <  2:42870/76‑1 (MQ=255)                                                                         |                                                                       CCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAA  >  REL606/3327043‑3327185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑