Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,489,218	T→A	intergenic (‑12/+5)	glpR ← / ← glpG	DNA‑binding transcriptional repressor/predicted intramembrane serine protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,489,218	0	T	A	100.0%	140.5 / NA	38	intergenic (‑12/+5)	glpR/glpG	DNA‑binding transcriptional repressor/predicted intramembrane serine protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (22/16);  total (22/16)

TGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGA  >  REL606/3489146‑3489290                                                                         |                                                                         tGACATAACCCTGCTGTTTTACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGa                                                                       >  1:195352/1‑76 (MQ=255)     aTAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAatta                                                                   <  2:690150/76‑1 (MQ=255)       aaCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAattatt                                                                 >  2:218085/1‑76 (MQ=255)           ctgctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTAttttcg                                                             >  1:365576/1‑76 (MQ=255)           ctgctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTAttttcg                                                             >  2:178165/1‑76 (MQ=255)            tgctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTAttttcgt                                                            >  2:549214/1‑76 (MQ=255)              ctgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTAttttcgttt                                                          >  1:509889/1‑76 (MQ=255)               tgTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTAttttcgtttt                                                         >  1:586562/1‑76 (MQ=255)                 tttAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTAttttcgttttcg                                                       >  1:406841/1‑76 (MQ=255)                  ttAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTcgc                                                      >  1:150702/1‑76 (MQ=255)                   tAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTcgcg                                                     >  1:230643/1‑76 (MQ=255)                      ccAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCAt                                                  <  2:710501/76‑1 (MQ=255)                        aGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTg                                                <  2:458136/76‑1 (MQ=255)                            cGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCg                                            <  1:478863/76‑1 (MQ=255)                              ataataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGaa                                          >  2:196635/1‑76 (MQ=255)                              ataataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGaa                                          <  2:598476/76‑1 (MQ=255)                              ataataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGaa                                          <  2:86387/76‑1 (MQ=255)                              ataataCCGTTGGGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGaa                                          <  2:199029/76‑1 (MQ=255)                               taataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                         <  1:439832/76‑1 (MQ=255)                               taataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                         <  1:431977/76‑1 (MQ=255)                                aataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATc                                        >  2:662484/1‑76 (MQ=255)                                      gTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaa                                  >  1:137310/1‑76 (MQ=255)                                        tgtgACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAg                                >  1:388598/1‑76 (MQ=255)                                          tgACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                              <  1:510315/76‑1 (MQ=255)                                          tgACGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                              >  1:466630/1‑76 (MQ=255)                                            aCGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGTGCGAATCAACAAAAGCCAt                            <  2:14695/76‑1 (MQ=255)                                            aCGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt                            <  2:221562/76‑1 (MQ=255)                                            aCGTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt                            <  2:548953/76‑1 (MQ=255)                                              gTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg                          >  1:151029/1‑76 (MQ=255)                                              gTTGTGTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg                          >  2:3172/1‑76 (MQ=255)                                                tgtgTTTGTTTCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                        >  2:341322/1‑76 (MQ=255)                                                   gtttgtttCATTTCTACATCCCAGGCATTATTTTCGTTTTCGCGCCTTGAGCGCATCACCAACAGCCCTCGCTaaa                     >  1:382908/1‑76 (MQ=255)                                                   gtttgtttCATTTCTAAATTCCAGGAATTCTTTTCGCTTTCGCGCATTGAGCTAATCAAAAACAGACATCGCtcac                     >  1:382849/1‑73 (MQ=255)                                                   gtttgtttCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGACTCCACAAAAGCCATCGCtcac                     >  1:382867/1‑73 (MQ=255)                                                   gtttgtttCATTTCTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa                     >  1:382896/1‑76 (MQ=255)                                                            aTTTCTAAATCCCAGGAATTATTGTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCt            <  2:619535/76‑1 (MQ=255)                                                                cTAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc        <  2:676891/76‑1 (MQ=255)                                                                 tAAATCCCAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAccc       <  2:291697/76‑1 (MQ=255)                                                                      cccAGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGa  >  1:118419/1‑76 (MQ=255)                                                                         |                                                                         TGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGA  >  REL606/3489146‑3489290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑