Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,498,019	G→A	T6I (ACA→ATA)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,498,019	0	G	A	100.0%	71.8 / NA	21	T6I (ACA→ATA)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/9);  total (12/9)

GTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACT  >  REL606/3497953‑3498088                                                                   |                                                                      gTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAg                                                              >  1:711124/1‑76 (MQ=255)      cATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTc                                                         >  2:752966/1‑76 (MQ=255)         cAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCata                                                      <  1:434097/76‑1 (MQ=255)            cTTTTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAg                                                   <  1:574603/76‑1 (MQ=255)             ttGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAgg                                                  >  1:710124/1‑76 (MQ=255)                  aGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGttt                                             <  2:709623/76‑1 (MQ=255)                      aTAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTg                                         >  1:580528/1‑76 (MQ=255)                      aTAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTg                                         <  2:576865/76‑1 (MQ=255)                         gagTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGaaa                                      <  1:349586/76‑1 (MQ=255)                                 aaGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTc                              >  1:84158/1‑76 (MQ=255)                                 aaGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTc                              >  2:565394/1‑76 (MQ=255)                                  aGGGCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCa                             >  2:675386/1‑76 (MQ=255)                                   gggCTTCTACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCaa                            <  2:218542/76‑1 (MQ=255)                                     gCTTCTACGCTAAGCGTGGGCGATGGATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGc                          <  1:591606/76‑1 (MQ=255)                                          tACGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAg                     >  2:214827/1‑76 (MQ=255)                                            cGCTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTa                   >  2:236600/1‑76 (MQ=255)                                              cTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTaaa                 <  1:731044/76‑1 (MQ=255)                                              cTAAGCGTGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTaaa                 >  1:287654/1‑76 (MQ=255)                                                     tGGGCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACg          >  2:366849/1‑76 (MQ=255)                                                       ggCGATGAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGt        >  2:42025/1‑76 (MQ=255)                                                             gAATATATAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACt  <  1:175732/76‑1 (MQ=255)                                                                   |                                                                      GTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACT  >  REL606/3497953‑3498088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑