| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,972 | (T)7→8 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,965 | 1 | . | T | 98.3% | 489.1 / 0.6 | 118 | intergenic (‑166/‑157) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (39/77); total (40/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
GAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAA > REL606/3911891‑3912034 | gAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACGTTCATACTGAAATTGAA‑t < 2:4833658/76‑1 (MQ=255) aaTGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tt > 1:4679948/1‑76 (MQ=255) tgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tttt > 1:4547348/1‑76 (MQ=255) cTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttttc > 2:3490008/1‑74 (MQ=255) cTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttttttttc < 1:5120635/76‑3 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactc < 1:3710897/76‑1 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactc < 2:4205440/76‑1 (MQ=255) aaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactcac > 2:2084070/1‑76 (MQ=255) aaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTtcactcac < 1:128520/76‑1 (MQ=255) aaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTcactcact < 1:1983025/76‑1 (MQ=255) aaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTcactcact < 2:1557258/76‑1 (MQ=255) aaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTcactcact < 1:953368/76‑1 (MQ=255) aaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTcactcact < 1:2424089/76‑1 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta < 2:3336199/76‑1 (MQ=255) aaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta < 2:4072239/76‑1 (MQ=255) aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtat < 1:4876661/76‑1 (MQ=255) aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtat < 2:520544/76‑1 (MQ=255) tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatt > 1:29173/1‑76 (MQ=255) tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatt < 2:893861/76‑1 (MQ=255) tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatt < 2:1580096/76‑1 (MQ=255) tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatt > 1:1677109/1‑76 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt < 1:2596028/76‑1 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt < 2:1492459/76‑1 (MQ=255) gCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattt < 2:4946880/76‑1 (MQ=255) cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtatttt < 2:1175736/76‑1 (MQ=255) gCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttat < 1:2038331/76‑1 (MQ=255) cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttatt < 1:1759561/76‑1 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttattt < 1:4602528/76‑1 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttattt < 2:182944/76‑1 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttattt > 1:4686343/1‑76 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACtattttattt < 2:3546379/76‑1 (MQ=255) gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATGCTGAAATTGAATTTTTTTTCACTCACTattttatttt < 1:2657724/76‑1 (MQ=255) gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTattttatttt < 1:3049554/76‑1 (MQ=255) aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTAttttt < 2:2449106/76‑1 (MQ=255) aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTAttttt > 2:1515499/1‑76 (MQ=255) aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTAttttt > 2:885303/1‑76 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaa < 1:4724171/76‑1 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaa > 2:3651299/1‑76 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaa < 1:2067676/76‑1 (MQ=255) aaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaa < 2:4597542/76‑1 (MQ=255) aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaa < 1:2232205/76‑1 (MQ=255) ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaaa < 1:384491/76‑1 (MQ=255) ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaaa > 1:3910933/1‑76 (MQ=255) gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaaaa < 2:3507217/76‑1 (MQ=255) gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTaaaaaa > 2:2423770/1‑76 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta < 2:4167774/59‑1 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACta > 1:4167774/1‑59 (MQ=255) tGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaaca < 1:3931481/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa < 1:2334769/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa > 1:2794292/1‑76 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa > 1:685278/1‑76 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa < 1:782050/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaa > 2:130131/1‑76 (MQ=255) aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAaacaac < 1:1833977/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca < 2:3815602/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca < 1:1993417/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca < 1:1182270/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAacaaca < 2:4185216/76‑1 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt > 1:4755927/1‑76 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt > 2:1186567/1‑76 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt > 2:2470273/1‑76 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt > 1:700114/1‑76 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt < 2:3157875/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt < 2:1764333/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAtt > 1:1988812/1‑76 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTta > 1:3548001/1‑76 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 1:1874190/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 2:3132188/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 2:3834065/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 1:3973356/76‑1 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 1:2794657/76‑1 (MQ=255) gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat < 1:352991/76‑1 (MQ=255) gAAGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat < 1:2720036/76‑1 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATAtt < 1:1126864/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg > 1:1194877/1‑76 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg < 1:3259885/76‑1 (MQ=255) ggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa > 2:1712726/1‑76 (MQ=255) ggAACAACATTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa > 2:1212984/1‑76 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt > 2:1403458/1‑76 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt < 2:1227225/76‑1 (MQ=255) caacaTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatta < 1:303883/76‑1 (MQ=255) acaTTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta < 1:3299186/76‑1 (MQ=255) caTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta < 2:1043220/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa < 2:3795714/76‑1 (MQ=255) tCATACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc > 1:2170843/1‑76 (MQ=255) aTACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGc > 1:3304542/1‑76 (MQ=255) tACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca > 2:3141903/1‑76 (MQ=255) tACTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca < 1:3427195/76‑1 (MQ=255) aCTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 1:372568/1‑76 (MQ=255) aCTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 2:4950272/1‑76 (MQ=255) aCTGAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 1:415543/1‑76 (MQ=255) gAAATTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:3199738/76‑1 (MQ=255) aaaTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa > 1:3495052/1‑76 (MQ=255) aaTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 2:674398/76‑1 (MQ=255) aaTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 2:2959792/76‑1 (MQ=255) aTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 2:2344672/76‑1 (MQ=255) aTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:4071796/76‑1 (MQ=255) aTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 1:323721/1‑76 (MQ=255) aTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:690130/76‑1 (MQ=255) aTTGAATTTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:2822241/76‑1 (MQ=255) | GAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAA > REL606/3911891‑3912034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |