| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,044,357 | 0 | A | C | 35.3% | 223.0 / 64.6 | 119 | V284G (GTG→GGG) | rfbD | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
| Reads supporting (aligned to +/- strand): ref base A (70/7); new base C (0/42); total (70/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-25 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 141 total aligned reads displayed.
CATCATGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGAC > REL606/2044282‑2044432 | catcatGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CAcaccca > 1:2273514/1‑76 (MQ=255) atcatGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CAcacccac > 1:4715407/1‑76 (MQ=255) atcatGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CAcacccac < 2:4134019/76‑1 (MQ=255) atcatGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CAcacccac > 1:1380853/1‑76 (MQ=255) atcatGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CAcacccac < 2:58336/76‑1 (MQ=255) tcatGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CAcacccacc < 2:4025262/76‑1 (MQ=255) catGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCt < 2:4971947/76‑1 (MQ=255) catGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCt > 1:2781004/1‑76 (MQ=255) catGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCt < 2:1884318/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTg > 2:5144207/1‑76 (MQ=255) atGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTg < 2:2606231/76‑1 (MQ=255) atGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTg > 1:4067582/1‑76 (MQ=255) tGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGc < 1:421170/76‑1 (MQ=255) tGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGc < 2:4889729/76‑1 (MQ=255) gAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGcc < 1:1494966/76‑1 (MQ=255) aGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCa > 1:3554795/1‑76 (MQ=255) gagaTGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CCCCCCCCCCTGCCAGTc < 2:841205/76‑1 (MQ=255) agaTGAAAAAATTTTTA‑AAATTGCCGT‑GGTCGTAAATAATTCGTGGAGCTTTCGTTT‑CACCCCCCCCTGCCAGTCa < 2:3265290/76‑1 (MQ=255) agaTGAAAAAATTGGTA‑AATTTGCCGT‑TGTCGTAAATAATTCTTTGAGCATTCGTTT‑CACCCCCCCCTGCCAGTCa < 2:3777956/76‑1 (MQ=255) tGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGc > 1:4926607/1‑76 (MQ=255) tGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGc > 2:4081278/1‑76 (MQ=255) cAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCaa > 2:4882139/1‑76 (MQ=255) aaaaatTTTTTT‑AAATTGCGGT‑AGTCGAAAATAATTCGTTGAGCTTTCGTTT‑CACCCCCCCCTGCCAGTCAGGCaa < 2:1536180/75‑1 (MQ=255) aaaatTTTGTT‑AAATTCCCGT‑AGTCGAAAATAATTCGTTGAGCATTCGTTT‑CACCCCCCCCTGCCAGTCAGGCAAg < 1:2650697/76‑1 (MQ=255) aaaaaTTTTTTAAAATTGCCGT‑AGTCGTAAAAAATTCGTTGAGCATTCGTTC‑CCCCCCCCCCTGCCAGTCAGGCaa < 2:3093491/76‑1 (MQ=255) aaaaaTTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAg > 2:4661082/1‑76 (MQ=255) aattttttt‑AAATTCCCGT‑AGTAGTAAATAATTCTTTGAGCTTTCGTTT‑CCCCCCCCCCTGCCAGTCAGGCAAGAc < 2:193517/73‑1 (MQ=255) aaaTTTTTT‑AAATTGCCGT‑AGTCGAAAATAATTCTTTGAGCATTCGTTT‑CACCCCCCCCTGCCAGTCAGGCAAGAc < 1:4235052/76‑1 (MQ=255) aaaTTTTTA‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACCCCCCCCTGCCAGTCAGGCAAGAc < 1:1056730/76‑1 (MQ=255) attttGTT‑AAATTCCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACCCCCCCCTGCCAGTCAGGCAAGACa < 2:4305172/74‑1 (MQ=255) attttGTA‑AAATTGCCGT‑AGTCGAAAATAATTCGTTGAGCTTTCGTTT‑CCCCCCCCCCTGCCAGTCAGGCAAGACa < 1:3911809/74‑1 (MQ=255) aaTTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACa > 2:3497762/1‑76 (MQ=255) ttttttt‑AAATTGCCGT‑ATTCGTAAATAATTCTTTGAGCATTCTTTT‑AACCCCCCCCTGCCAGTCAGGCAAGACaa < 1:4133633/75‑1 (MQ=255) tttttt‑AATTGGCCGT‑AGTCGAAAATAATTCGTTGACCATTCTTTC‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAg < 1:2124162/76‑1 (MQ=255) tttGTT‑AATTTGCCGT‑AGTGTTAAAGATTTCGTTAACCATTCTTTT‑CACCCCCCCCTGCCAGTCAGGCAAGACAAg < 2:2136023/76‑1 (MQ=255) tttGTA‑AAATGCCCGT‑AGTCGAAAATATTTCGTTGAGCTTTCTTTC‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAg < 1:1845222/76‑1 (MQ=255) ttttt‑AATTGGCCTT‑AGTCGTAAATATTTCGTTGAGCTTTCTTTT‑CACCCCCCCCTGCCAGTCAGGCAAGACAAgc < 2:3760933/73‑1 (MQ=255) ttttt‑AAATTTGCGTAAGTCGAAAATATTTCGTTGAGCATTCTTTC‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAg < 2:2924032/73‑1 (MQ=255) ttGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAgc > 1:1109009/1‑76 (MQ=255) ttGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAgc > 1:1803768/1‑76 (MQ=255) ttGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAgc > 1:2299856/1‑76 (MQ=255) tGTT‑AAATTGCGGT‑ATTCGTAAATAATTCTTTGAGCTTTCGTTC‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAgcg < 2:3694433/76‑1 (MQ=255) tGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAgcg > 1:1192264/1‑76 (MQ=255) tGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAgcg > 2:1982593/1‑76 (MQ=255) tGTA‑AATTTGCCGT‑ATTCGTAAATATTTCTTTGAGCATTCGTTT‑CACCCCCCCCTGCCAGTCAGGCAAGACAAgcg < 2:4425793/76‑1 (MQ=255) gTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAgcgc > 2:4139506/1‑76 (MQ=255) aaaTTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCaaa > 1:2604834/1‑76 (MQ=255) atttGCCGA‑AGCCGAAAATATTGCGTTGAGCATTCGTTT‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAg < 2:2831877/74‑1 (MQ=255) aTTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGt > 2:4781161/1‑76 (MQ=255) tGCCGT‑AGTCGTAAATAATTCGTTTAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTc > 1:3375566/1‑76 (MQ=255) gT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctgct > 1:2917811/1‑76 (MQ=255) tattCGTAAATAATTCGTTGAGCATTCGTTC‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTctgctg < 1:1982593/73‑1 (MQ=255) aGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGa > 1:2249554/1‑76 (MQ=255) aGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACACACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGa > 2:1642254/1‑76 (MQ=255) tCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaaa > 1:2061231/1‑76 (MQ=255) ggttAAATATTTCTTTGAGC‑CTCGTTTCCCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGaaa < 1:3241029/73‑1 (MQ=255) cGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAAc > 2:3275077/1‑76 (MQ=255) cGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAAc > 2:4614239/1‑76 (MQ=255) cGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAAc > 2:223591/1‑76 (MQ=255) gTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACt > 2:1772562/1‑76 (MQ=255) aaaaTAATTCGTTGAGCATTCGTTT‑CACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtt < 2:2660581/75‑1 (MQ=255) aataatTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACtttt > 1:2189753/1‑76 (MQ=255) taatTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCt > 1:2441734/1‑76 (MQ=255) taatTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCt > 2:4426074/1‑76 (MQ=255) taatTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCt > 1:4532005/1‑76 (MQ=255) aatTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTg > 2:903502/1‑76 (MQ=255) atTCTTTGAGCTTTCTTTC‑CACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGt < 2:2798151/76‑1 (MQ=255) ttCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTa > 1:4487831/1‑76 (MQ=255) cGTTGAGCATTCGTTT‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt < 2:1754420/76‑1 (MQ=255) cGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTAtt > 2:1409328/1‑76 (MQ=255) tttGATCATTCTTTC‑CACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTg < 1:4728406/75‑1 (MQ=255) gtggAGCTTTCGTTC‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTg < 1:3196705/73‑1 (MQ=255) ttGGGCTTTCTTTT‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGa < 2:882633/76‑1 (MQ=255) ttGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGa > 1:1652955/1‑76 (MQ=255) ttGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGa > 2:2745703/1‑76 (MQ=255) tGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAg > 1:1971212/1‑76 (MQ=255) gaccATTCGTTC‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAgg < 1:404315/73‑1 (MQ=255) gAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAgg > 2:2672574/1‑76 (MQ=255) gAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAgg > 1:458155/1‑76 (MQ=255) aGCATTTGTTC‑CCCCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGc < 1:625645/76‑1 (MQ=255) cATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCga > 1:1614972/1‑76 (MQ=255) cATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCga > 1:1707547/1‑76 (MQ=255) cATTCGTTC‑CACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCga < 1:5170180/76‑1 (MQ=255) aTTCTTTT‑CACCCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgag < 2:3080622/76‑1 (MQ=255) aTTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCgag > 2:4634955/1‑76 (MQ=255) cGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGt > 1:877367/1‑76 (MQ=255) cGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGt > 2:4799129/1‑76 (MQ=255) cGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGCTCTGCTGAAACTTTTCTGCATTGAGGCGAGAGt > 1:3037509/1‑76 (MQ=255) gTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGtt > 1:3528637/1‑76 (MQ=255) ttt‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTa > 2:2249620/1‑76 (MQ=255) ttt‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTa > 1:4810568/1‑76 (MQ=255) tt‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTAt > 1:2479109/1‑76 (MQ=255) tccacaCCCCCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTAt < 2:3441579/74‑1 (MQ=255) t‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAATCTTTTCTGTATTGAGGCGAGCGTTCTg > 2:1261005/1‑76 (MQ=255) cacaCCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATgg > 2:8373/1‑76 (MQ=255) cccccccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGt < 1:935393/75‑1 (MQ=255) ccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCg > 1:4154624/1‑76 (MQ=255) ccaccTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCg > 1:4880087/1‑76 (MQ=255) accTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGAc > 2:1368661/1‑76 (MQ=255) accTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGAc > 2:1594396/1‑76 (MQ=255) | CATCATGAGCGAGATGCAAAAATTTGTT‑AAATTGCCGT‑AGTCGTAAATAATTCGTTGAGCATTCGTTT‑CACACCCACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAACTTTTCTGTATTGAGGCGAGAGTTATGGGGTCGAC > REL606/2044282‑2044432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |