| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 37.5% | 144.3 / 17.9 | 89 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (11/44); new base G (33/0); total (45/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.12e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 117 total aligned reads displayed.
CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 | cACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtg > 1:2454786/1‑76 (MQ=255)cACCAGATGAGGCGAACTGTAGACGCCCACTTAGGACCCTGCCGCCATCAGAATCGACTCCAGCGAACGGCAggtg < 2:2542984/76‑1 (MQ=255) aCCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggggg > 1:1344476/1‑76 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtggt < 1:329121/76‑1 (MQ=255) cAGATGAGGCGAACTGTAGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGgtggtg < 2:3347330/76‑1 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAgggggggg > 1:4347362/1‑73 (MQ=255) aGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGc > 2:3888964/1‑76 (MQ=255) gATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGcc > 1:1812466/1‑76 (MQ=255) aggAGGCGAACTGTAGACGCCCCTTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCt < 2:718175/74‑1 (MQ=255) aTGAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCt < 2:4912898/76‑1 (MQ=255) aTGAGCCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCt < 2:2638053/76‑1 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCtt > 2:3874062/1‑76 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGccct > 2:1005174/1‑74 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGCGGGGCCtt > 2:1921738/1‑76 (MQ=255) tGAGGCGAACGGTAGCCCCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCtt < 2:2344835/76‑1 (MQ=255) tGAGGCGAAATGTAGACGCCCATTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCtt < 2:458378/76‑1 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCttt > 1:1253950/1‑76 (MQ=255) gAGGCAAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 2:177328/76‑1 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTg < 2:3815379/76‑1 (MQ=255) ggCGACCTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGc < 2:624350/76‑1 (MQ=255) ggCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGc < 1:4970996/76‑1 (MQ=255) ggCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCctttg > 1:1923513/1‑74 (MQ=255) aaCTGTAGCCGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCAtt < 1:2940356/76‑1 (MQ=255) aaCTGTAGACCCCCCCTTTGTCCCCGGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCAtt < 1:2871120/76‑1 (MQ=255) cTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGGCTTTGCCATTCg > 2:4092131/1‑76 (MQ=255) cTGGAGACGCCCCCTTGGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCg < 1:3425114/76‑1 (MQ=255) tttAGACCCCCACTTTTTACCTTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGt < 2:5050901/74‑1 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCCTTGCCCTTTGTa > 1:16592/1‑76 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGCCCTTCGTa > 1:1075379/1‑76 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTTCCATTCGTAc > 1:3012453/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTAcc > 1:4190736/1‑76 (MQ=255) aGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGGCATTTGTaac > 1:1601009/1‑74 (MQ=255) gccGCCCCCTTTGCCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAccc < 1:4166693/74‑1 (MQ=255) gACGCCCATTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAccc < 1:709991/76‑1 (MQ=255) gACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTAccc < 1:5172601/76‑1 (MQ=255) gACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGGCCTTCGTAccc > 1:1142676/1‑76 (MQ=255) cGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCTTTTCCATTCGTTCCCGc > 2:3205372/1‑76 (MQ=255) gCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGGCTTTGCCATTCGTACCCGcc > 2:4075670/1‑75 (MQ=255) ccccTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAAc < 1:1423202/73‑1 (MQ=255) ccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGACATTCGTACCCGCCAc > 2:1178626/1‑76 (MQ=255) cACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGGCTTTGCCATTCGTACCCGCAACg > 1:3708073/1‑76 (MQ=255) acgtttGTCCCCTGCCTCCATCAGAATCGACTCCAGCGTCAGGAAGGTGGTCCCTTTGCCATTCGTACCCGCAACg < 2:1152285/73‑1 (MQ=255) aCTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTAACCCCAACgg > 1:450706/1‑76 (MQ=255) cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGACATTCGTACCCGCAACgga > 2:1829645/1‑75 (MQ=255) cTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCCCAACGGt > 2:4325917/1‑76 (MQ=255) cTTTGCCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGt < 1:3944261/76‑1 (MQ=255) tttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGGCATTCGTACCCGCAACGGTa > 2:641895/1‑76 (MQ=255) tttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACggga > 1:1795507/1‑74 (MQ=255) ttGTCCCCGCCCGCCATCAGAATCGACCCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTaa < 2:3098097/76‑1 (MQ=255) ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGACTTTGGCATTCGTACCCGCCACGGGaa > 1:606609/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGGCATTCGTAACCGCAAAGGGaaa > 2:1709142/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTaaa > 1:1208409/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGaaa > 2:3623165/1‑76 (MQ=255) tccccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 1:4547458/74‑1 (MQ=255) tccccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAaca < 1:1175763/74‑1 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGCCGCTGCCCTTCGTACCCGCACCGGTTAaca > 1:4986627/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCATTCGTACCCGCCACGGTAAaca > 1:4172607/1‑76 (MQ=255) tACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCCTTGGCATTCGGACCCCCAACCGGaaaac > 1:794912/1‑74 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCCACGGGAAacaa > 1:3007401/1‑75 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCCCAACGGGAAacac > 1:883640/1‑76 (MQ=255) cccTCCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAAcaca < 1:515984/76‑1 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaaa > 2:2551617/1‑76 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCCACGGGAAACACaaa > 1:735523/1‑76 (MQ=255) tGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGCCATTCGTACCCGCAACGGTAAACACaaaa > 2:875863/1‑75 (MQ=255) cgccATCCGAATCGGCTCCAGCGTCCGGCGGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGgc < 2:3720734/76‑1 (MQ=255) cgccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGgc > 2:3875335/1‑76 (MQ=255) ccATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGgcgc > 2:4213158/1‑76 (MQ=255) caccAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCt < 2:1781057/73‑1 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCt > 2:3708592/1‑76 (MQ=255) cATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAAAGGTAAAAACAAATGgggcg > 1:523570/1‑75 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTg < 1:3871785/76‑1 (MQ=255) gAATCGCCTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttt < 2:2984296/76‑1 (MQ=255) gAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCATTCGTAACCGCCACGGTAAAAAAAAATGGCGCTGGgtt > 2:2968577/1‑76 (MQ=255) aaccGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTc < 2:292846/73‑1 (MQ=255) aaTCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTc < 1:773495/76‑1 (MQ=255) aaTCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTc < 1:3930662/76‑1 (MQ=255) aaTCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTc < 1:1408523/76‑1 (MQ=255) agcGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 2:2121158/74‑1 (MQ=255) agcGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCa < 1:1982174/74‑1 (MQ=255) cGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTTCAgg > 1:1439831/1‑76 (MQ=255) gACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGa < 2:4844371/76‑1 (MQ=255) aCTCCAGCGTACGGCAGGTGGTGCCTTTGACATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGAc > 2:3913918/1‑76 (MQ=255) ccAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAAAACAAATGGCGCTGGTTTCAGGACGcc > 2:2777720/1‑76 (MQ=255) cAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCa < 2:1899239/76‑1 (MQ=255) aGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTTCAGGACGCCaa > 2:3972257/1‑76 (MQ=255) gCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:4972198/76‑1 (MQ=255) cGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGa < 1:2696889/76‑1 (MQ=255) cGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAACAAAAATGGCGCTGGGTTTTGGATGCCCAGa > 2:952784/1‑76 (MQ=255) tccGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcg < 2:3940578/74‑1 (MQ=255) gggCAGGTGGTGCTTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg < 2:4300622/75‑1 (MQ=255) gggCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg < 1:270927/75‑1 (MQ=255) cGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg > 2:454534/1‑76 (MQ=255) cGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg > 2:4317287/1‑76 (MQ=255) cGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcgcg > 2:1834707/1‑76 (MQ=255) cGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGGCGCTGGGTTCAGGACGCCCAGAagcc > 2:2159731/1‑75 (MQ=255) ggCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACgcgc > 1:1496569/1‑76 (MQ=255) ggCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACgcgc > 2:5064369/1‑76 (MQ=255) cAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACgcgctg < 2:1511264/76‑3 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg > 1:213191/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 2:253596/1‑76 (MQ=255) | CACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376274‑2376423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |