| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,096,354 | 0 | A | C | 34.9% | 131.4 / 39.4 | 83 | V163G (GTG→GGG) | sufI | repressor protein for FtsI |
| Reads supporting (aligned to +/- strand): ref base A (50/4); new base C (0/29); total (50/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-18 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 114 total aligned reads displayed.
TCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGA > REL606/3096279‑3096428 | tCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTtca > 1:1444089/1‑76 (MQ=255)tCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTcca > 1:276132/1‑76 (MQ=255) ccTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTccac < 2:2892161/76‑1 (MQ=255) cTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTccccc > 2:2525013/1‑76 (MQ=255) tGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTccccca > 1:3214792/1‑76 (MQ=255) tGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTccacca > 2:1082425/1‑76 (MQ=255) tGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTccacca < 2:2722361/76‑1 (MQ=255) ggATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAg < 2:1270306/76‑1 (MQ=255) gATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGc > 1:2785268/1‑76 (MQ=255) gATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGc > 2:5155200/1‑76 (MQ=255) gATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATACGCAGAGACTTACTGCCTTCATTTTtcaccata > 2:4408408/1‑74 (MQ=255) aaTGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCcac > 2:1482305/1‑76 (MQ=255) aTGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCcaca > 2:2014605/1‑76 (MQ=255) tGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCCTCTTCCACCAGCCACAt > 2:281013/1‑76 (MQ=255) tGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACAt < 1:3731410/76‑1 (MQ=255) gACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACAtt > 1:2476040/1‑76 (MQ=255) gccGAAAAATCCTCCACCCCAAAAGGTTGGGGGATAGGCACCGACTTCCTTACTTCATCTCCCCCCAGCCACATTc < 2:2936680/75‑1 (MQ=255) ccGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATTTTCCACCAGCCACATTcc > 1:471106/1‑76 (MQ=255) caaaaaaCCACCCACCCAAAAAGGGTGGGGGATGGGCGGCGACTTTCTGTCTTCTTCTCCCCCCAGCCACATTCCg < 2:4766829/73‑1 (MQ=255) aaaaCCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCCCCAGCCACATTCCGGCa < 1:1002322/76‑1 (MQ=255) aaaTCATCCACACCATAATGGTTGGGGATAGGCAGCGGCTTGCCTGCTTCATCTTCCAGCAACCACATTCCCGCaa > 1:1319428/1‑76 (MQ=255) atcatcCACACCATAATGGTTGGGGTTAGGCAGCGACTTGCTAGCTTCATCTTCCACCATCCACACTTCGGCAAgg > 1:4169600/1‑76 (MQ=255) atcatcCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCCCCAGCCACATTCCGGCAAgg > 1:681368/1‑76 (MQ=255) atcatcCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAgg > 1:126521/1‑76 (MQ=255) cccccACACAATAATGGTGGGGAATAGGCAGCTCCTTGCTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGcc < 2:4200982/73‑1 (MQ=255) catcCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGcc > 2:3222921/1‑76 (MQ=255) ccACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCCTTTTCCACCAGCCACATTCCGGCACGGCCGtt > 1:220777/1‑76 (MQ=255) ccACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGtt > 1:4570358/1‑76 (MQ=255) ccACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGtt > 1:1807071/1‑76 (MQ=255) ccACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGtt > 1:1261228/1‑76 (MQ=255) cacaCCATAAGGGTGGGGGATAGGCAGCGACTTGCTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTta < 1:4789423/76‑1 (MQ=255) accccATAAGGGTTGGGGAAAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTtat < 1:4407856/73‑1 (MQ=255) ccccATAAGGGTGGGGGAGAGGCAGCGTCTGGCGCACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTtata < 2:1758736/74‑1 (MQ=255) cacCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTtata > 1:1820162/1‑76 (MQ=255) cccAAAAGGGGTGGGGAGAGGCGGCGTCTTGCTGTCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAg < 1:1213235/75‑1 (MQ=255) acCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAg > 1:4940885/1‑76 (MQ=255) ccATAATGGTTGGGGATAGGCAGCGACTTGCCGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGa > 2:279983/1‑76 (MQ=255) caaTAAGGGTGGGGAATAGGCAGCGCCTTGCTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGa < 1:1328992/74‑1 (MQ=255) cATAATGGTTGGGGATAGGCAGCGACTTGCTTACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGGTATAGAc > 1:1674775/1‑76 (MQ=255) cATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGAc < 2:3416198/76‑1 (MQ=255) cATAAGGGTGGGGGAGAGGCAGCGACTTCCTGACTTCATCTTCCCCCAGCCACATTCCGGCAAGGCCGTTATAGAc < 2:3716038/76‑1 (MQ=255) aaTAAGGGTGGGGGATGGGCAGCGACTTGCTGATTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGAc < 2:2277582/75‑1 (MQ=255) aTAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGAcc > 1:3960026/1‑76 (MQ=255) aTAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCAACAGCCACATTCCGGCAAGGCCGGTATAGAcc > 1:1834991/1‑76 (MQ=255) aTAATGGTTGGGGATAGGCAGAGACTTGCTGACTTCATCTTCCGCCAGCCACATTCCTGCAATGCCGTTATacaca > 2:4826837/1‑75 (MQ=255) aTAAGGGTGGGGGAGAGGCAGCGCCTTGCTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGAcc < 1:2872699/76‑1 (MQ=255) tAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACct > 2:3579299/1‑76 (MQ=255) aaTGGTTGGGGATAGGCAGCGACTTTCTGACTTCATCTTCCACCAGCCACAATCCGGCAAGGGCGTTTTAGACctg > 1:1794159/1‑76 (MQ=255) aaTGGTTGGGGATAGGCAGCGACTTGCTGACTTCCTCTTCCACCAGCCACATTTCGGCAAGGCCGTTTTAGGCctg > 2:1398274/1‑76 (MQ=255) aaTGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACctg > 1:4466050/1‑76 (MQ=255) aaTGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACctg > 2:3318752/1‑76 (MQ=255) tggtggGGGATAGCCAGCGGCTTGCTGCCTTCATCTTCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACctgct < 1:2832346/76‑1 (MQ=255) ggTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACctgctg > 1:3295140/1‑76 (MQ=255) ttGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAg > 2:1595030/1‑76 (MQ=255) tgggggAGGGGCCGCGTCTTGCTGCCTTCTCCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAg < 1:4667142/74‑1 (MQ=255) tgggggAGAGGCAGCGACTTGCTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAg < 1:352158/74‑1 (MQ=255) tGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGGTATAGACCTGCTGGGc > 2:1807963/1‑76 (MQ=255) tGGGGATAGGCAGCGACTTGCTGACTTCATCTCCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGc < 1:2444091/76‑1 (MQ=255) ggggATAGGCAGCGACTGGCTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGcc < 2:1441246/76‑1 (MQ=255) gggATAGGCAGAGACTTCCGGACTTCTTCTTCCACCACCCACTTTCTGGCCACGCCGTTTTAGACCTGCTGAGCCg > 2:3986237/1‑76 (MQ=255) ggATAGGCAGCGACCTGCTGACTTCATCTTCCACCAGCCACATTCCGGCCAGGCCGTTATAGACCTGCTGAGcccg > 2:1308608/1‑74 (MQ=255) aTAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTTCGGCAAAGGCGTTTTAGAACCGCGGAGGCgcgg > 2:4339564/1‑73 (MQ=255) aGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCgg > 1:2348562/1‑76 (MQ=255) ggCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGt > 1:2294280/1‑76 (MQ=255) gCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGtt > 2:416046/1‑76 (MQ=255) cAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTg > 2:3668884/1‑76 (MQ=255) cAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTg > 2:2620870/1‑76 (MQ=255) cAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCAGTGCGGTTg > 2:5108610/1‑76 (MQ=255) aGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTgg > 2:2734690/1‑76 (MQ=255) gCGATTTGCTGACTTCCTCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTggg < 2:1456719/76‑1 (MQ=255) gCGACTTGCTGCCTTTCTCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTggg < 2:1854365/76‑1 (MQ=255) gtcTTGCTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAg < 2:141919/74‑1 (MQ=255) gctttGCTGCCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAg < 2:2679041/73‑1 (MQ=255) gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGGGCCGTGCGGGTGGGAg > 1:3203490/1‑76 (MQ=255) gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAg > 1:1035692/1‑76 (MQ=255) cTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTa > 2:3075957/1‑76 (MQ=255) cTTGCTGACTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTa < 2:2389526/76‑1 (MQ=255) ttGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTAt > 1:3701967/1‑76 (MQ=255) ttGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTAt > 1:2177253/1‑76 (MQ=255) tGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTAtt > 1:1996349/1‑76 (MQ=255) gCTGCCTTCCTCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTg < 2:4113611/76‑1 (MQ=255) cTGATTTCTCCTCCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTgg < 2:5033875/76‑1 (MQ=255) cTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTgg > 1:4557711/1‑76 (MQ=255) tgccTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGc < 1:1038438/73‑1 (MQ=255) gACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCg > 1:3396261/1‑76 (MQ=255) aCTTCTTCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGt < 1:2468976/76‑1 (MQ=255) tttCCTCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTg < 1:2713395/75‑1 (MQ=255) cttcttCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTg < 1:621519/76‑1 (MQ=255) cTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTg > 1:2871145/1‑76 (MQ=255) tCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGAt > 2:3052262/1‑76 (MQ=255) tCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGAt > 2:4642488/1‑76 (MQ=255) tCATCCCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGAt < 1:1938208/76‑1 (MQ=255) cATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATa > 1:3776193/1‑76 (MQ=255) cATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATa > 1:3312423/1‑76 (MQ=255) tCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATAGcaca > 1:4007923/1‑76 (MQ=255) tCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACcaca > 1:4111590/1‑76 (MQ=255) ccaccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACag > 1:4313780/1‑76 (MQ=255) ccaccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACag > 2:5067592/1‑76 (MQ=255) caccaGCCACATTCCGTCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACaga > 1:1893940/1‑76 (MQ=255) caccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGCTACCACaga > 2:4479460/1‑76 (MQ=255) | TCCTGGATAATGACCGGAAAATCATCCACACCATAATGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGA > REL606/3096279‑3096428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |