| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,378,885 | 0 | T | G | 41.1% | 246.1 / 55.6 | 141 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
| Reads supporting (aligned to +/- strand): ref base T (13/70); new base G (58/0); total (71/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.36e-27 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 168 total aligned reads displayed.
GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 | tGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGgtgt < 2:1758592/76‑1 (MQ=255) gACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGtt < 2:4691270/76‑1 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAc < 1:2806223/76‑1 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTAc < 2:2227122/76‑1 (MQ=255) ccGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTAc > 2:3832070/1‑76 (MQ=255) gCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑t < 2:1157395/76‑1 (MQ=255) ccTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑Tg < 1:236642/76‑1 (MQ=255) cTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑Tgg > 1:4286261/1‑76 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCC‑TGGt > 1:1694216/1‑76 (MQ=255) tttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTTCC‑TGGt > 1:4503783/1‑76 (MQ=255) ttACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGtt < 2:1984853/76‑1 (MQ=255) tACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGttt < 1:4240013/76‑1 (MQ=255) tACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTTAC‑TGGttt > 1:3545776/1‑76 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTg < 1:597537/76‑1 (MQ=255) aCCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTg > 2:2009959/1‑76 (MQ=255) ccTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGa < 1:4813973/76‑1 (MQ=255) ccTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACCTTGGttta > 2:844116/1‑75 (MQ=255) ccTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGa > 2:545636/1‑76 (MQ=255) ccTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGTTTGa > 1:512528/1‑76 (MQ=255) cTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGAt > 2:752756/1‑76 (MQ=255) tGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGGTTGAtt > 1:1776613/1‑76 (MQ=255) ggTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTg < 1:5136029/76‑1 (MQ=255) ggTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTTCC‑TGGGTTTATTg > 1:4347672/1‑76 (MQ=255) ggTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTTCC‑TGGCTTGGTTg > 1:4160158/1‑76 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTgcagc > 2:1130459/1‑76 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTgcagc > 2:2843887/1‑76 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGGTTGATTgcagc > 1:45354/1‑76 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGGTTGATTgcacg > 2:1060262/1‑74 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGTTTTATTtcagc > 1:1382296/1‑76 (MQ=255) tCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGTTTGATTggagc > 2:1442670/1‑76 (MQ=255) cATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTAGC‑CGGTTTTATTgtagca > 1:1346351/1‑76 (MQ=255) tttCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATc < 1:2995065/76‑1 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAAc < 1:3235913/76‑1 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTTC‑TGGGTTGATTTCAGAATTaaa > 1:2099669/1‑75 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCC‑TGGGTTGGTTTCAGCATCAAc > 1:4699511/1‑76 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAAc > 1:1858647/1‑76 (MQ=255) cTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGTTTGATTGCAGGATCAAc > 1:1002894/1‑76 (MQ=255) aCGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGc > 2:4168703/1‑76 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTTGTTGCAGCATCAACGcc > 2:874418/1‑76 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGGTTTATTGCAGCATCAAAGcc > 1:829208/1‑76 (MQ=255) cGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGGTTGAATGCAGCATCCACGcc > 2:2633916/1‑76 (MQ=255) gTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCa > 2:5148893/1‑76 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAg < 1:2518307/76‑1 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAg < 1:2583187/76‑1 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTTCC‑TTGTTTCGTTGCAACACTAACGccca > 2:2850640/1‑74 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAg > 1:2523295/1‑76 (MQ=255) ttATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGGTTGATTGCAGCATCAACGCCAg > 1:1164271/1‑76 (MQ=255) ttATGAACAGTAGAAACAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTgc < 1:3562940/62‑1 (MQ=255) ttATGAACAGTAGAAACAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTgc > 2:3562940/1‑62 (MQ=255) tATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGa < 1:2660523/76‑1 (MQ=255) tATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTTATTGCAGCATCCACGCCAGa > 1:2885963/1‑76 (MQ=255) tATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGGTTGATTGCAGCATCAACGCCAGa > 2:623648/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGGTTGATTGCAGCATCAACGCCAGAc > 1:4995766/1‑76 (MQ=255) aTGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGGAAC‑TGGGGTGATTGCAGACACAACGCCAGAc > 1:319566/1‑76 (MQ=255) tGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACt < 1:3080151/76‑1 (MQ=255) tGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGCTTGAATGGAGCACTAAagacagaca > 2:3227479/1‑75 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAAt > 1:1467909/1‑76 (MQ=255) cAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGGTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAAt > 1:3978643/1‑76 (MQ=255) tAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAAACCCAGACTGAATCTg > 2:3374709/1‑76 (MQ=255) tAGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTAAC‑TGGTTTGGTTGCAGCATCAACGCCAGACTGCATCTg > 2:13678/1‑76 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTgg > 2:1632539/1‑76 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTgg > 1:956850/1‑76 (MQ=255) aGAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGGTTGATTGCAGCATCAACGCCAGACTGAATCTgg > 1:926935/1‑76 (MQ=255) gAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGt < 2:4575696/76‑1 (MQ=255) gAACCAACCGGGATGTTGCGCATCGGCAGGGGGTTACC‑CGGGTTGGTTGCAGCATCAACGCCAGACTGGATCTggg > 2:1435316/1‑75 (MQ=255) aaccaaccGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTc < 1:449005/76‑1 (MQ=255) accaaccGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCg < 1:4703681/76‑1 (MQ=255) ccaaccGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGgcgc > 1:2274147/1‑76 (MQ=255) ccaaccGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGc > 2:4001020/1‑76 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc < 1:3227479/76‑1 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCCGACTGAATCTGGTCGcc > 1:1060327/1‑76 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc > 1:2036666/1‑76 (MQ=255) caaccGGGATGTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGcc > 2:445744/1‑76 (MQ=255) aaccGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCa < 2:3472817/76‑1 (MQ=255) accGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAg < 1:776640/76‑1 (MQ=255) accGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAg < 1:49396/76‑1 (MQ=255) ccGGGATGTTGCGCATCGGCAGGGGGTTACC‑TTGTTTGATTGCAGCATCAACGCCAGCCTGAATCTGTGCTCCAGc > 2:2400845/1‑76 (MQ=255) ggATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCttt < 2:4884289/76‑1 (MQ=255) gATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTc < 2:639874/76‑1 (MQ=255) gATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTc < 1:2764087/76‑1 (MQ=255) tGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAg < 1:3543319/76‑1 (MQ=255) tGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAg < 1:1656555/76‑1 (MQ=255) gTTGCGCATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTTAgg > 2:3644912/1‑76 (MQ=255) gcATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCttt < 1:1485002/76‑1 (MQ=255) gcATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCttt < 1:3420068/76‑1 (MQ=255) cATCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGGCAGCTTTCAGGCCtttt > 2:2288824/1‑75 (MQ=255) aTCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAg < 1:1435316/76‑1 (MQ=255) tCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAgg < 2:1909741/76‑1 (MQ=255) tCGGCAGGGGGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGGCTTTAgg > 2:4835631/1‑76 (MQ=255) cGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAggg > 1:3348255/1‑76 (MQ=255) cGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAggg > 1:2126410/1‑76 (MQ=255) cAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGcc > 2:4200654/1‑76 (MQ=255) aGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:810956/1‑76 (MQ=255) aGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:918607/1‑76 (MQ=255) aGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa > 2:1696619/1‑76 (MQ=255) aGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCa < 2:3298855/76‑1 (MQ=255) ggTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAgg < 2:5081286/76‑1 (MQ=255) ggTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAgg > 2:773017/1‑76 (MQ=255) tgtTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt > 2:4675147/1‑76 (MQ=255) tgtTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAt > 1:4403468/1‑76 (MQ=255) | GCTGACCGCCTTTACCTGGTTTCATTTCTACGTTATGAACAGTAGAACCAACCGGGATGTTGCGCATCGGCAGGGTGTTACC‑TGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGAT > REL606/3378810‑3378960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |