| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,392,556 | 0 | C | T | 41.8% | 90.5 / 72.6 | 91 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (39/14); new base T (0/38); total (39/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 146 total aligned reads displayed.
AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 | aGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTc < 2:4094698/76‑1 (MQ=255) gCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCt > 2:4758819/1‑76 (MQ=255) gCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCt < 2:3625311/76‑1 (MQ=255) gCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCt < 1:3343045/76‑1 (MQ=255) gCAACTTATACCTGTCTGATGGCGCGCTCTGCAAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCt < 2:259671/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGAAAGCGATTTTTTa > 2:4139269/1‑76 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg > 1:2469174/1‑76 (MQ=255) aCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGc < 2:3988746/76‑1 (MQ=255) aCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGACTTTCTAGc > 1:1055859/1‑76 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGGTTTTCTAGcc > 1:135900/1‑76 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAggc > 1:1612444/1‑74 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 1:5165116/76‑1 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 2:3901211/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGGGGTTTTTTAGCCa > 1:964057/1‑76 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCa > 1:1539092/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGcccg > 1:2908187/1‑74 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAACCAg > 1:2775108/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg > 1:1126022/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg > 2:1073754/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg < 2:1539092/76‑1 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGt > 1:2826941/1‑76 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGt > 1:3879380/1‑76 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGt > 2:2875418/1‑76 (MQ=255) tcccTGTCTGAGGGCGCGCTCGGGGAAGCGGTAACGGGTGAAGGTGCTGGGGGGGATAGCTTTTTTTTAGCCAGTa < 2:4778875/74‑1 (MQ=255) taCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTa > 1:1311683/1‑76 (MQ=255) aCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTAt > 2:4374287/1‑76 (MQ=255) aCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTAt > 2:2620354/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGGATTg > 1:4658868/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCTTTTTTTTAGCCAGTATTg < 2:695381/76‑1 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGCGCTGGCTGGGATAACGATTTTCTAGCCAATATTg > 1:4818126/1‑76 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTgg > 1:1493838/1‑76 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGAGCTGGCTGGGACAGCGATTTTCTTGCCACTATTgc > 2:941640/1‑75 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGGTTTTCTAGCCCGTTTTGGt > 2:2208998/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGt > 1:3758499/1‑76 (MQ=255) gcggATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTc < 2:139765/73‑1 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTTGCCAGTATTGGttc > 1:1766813/1‑74 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTca > 1:192404/1‑76 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGCTTTTCTACCCAGTAATGGTca > 2:960604/1‑76 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGTATGCCACTTTCTAGGCAGTATTGGTccg > 1:1761511/1‑74 (MQ=255) ggATGGCGCCCTGTGCGAAGCGTTAACGCGTGAGGGTGCTGGCTGGGATAGCTATTTTTTAGCCAGTATTGGTcag < 2:4898095/75‑1 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTcagc > 2:4378267/1‑76 (MQ=255) aTGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTcagca > 1:2485611/1‑76 (MQ=255) gggCGCGCTCTGGGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTcagcag < 2:2020557/75‑1 (MQ=255) gcgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGtt > 1:3594935/1‑76 (MQ=255) gcgcgcTCGGCGAAGCGGTAGCGCGTGAGGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGtt < 1:4720185/76‑1 (MQ=255) gcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAg < 2:2385512/76‑1 (MQ=255) gcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAg > 2:3478353/1‑76 (MQ=255) gcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGGATTTGTCAGCAATTAg > 2:4023325/1‑76 (MQ=255) gcgcTCGGCGAAGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAg < 2:458085/76‑1 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGGCACTATTGGTCAGCGGTTatg > 2:2820806/1‑74 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGAGAGGGGTTTTCTAACCAGTATTGGTCAACAGTTTgg > 1:1872315/1‑76 (MQ=255) gcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGa > 2:35358/1‑76 (MQ=255) ctctGCGAAGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:71646/76‑1 (MQ=255) ctcgGCGAGGCGGAAACGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:4505316/76‑1 (MQ=255) tctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTTAGCAGTTAAGAAc > 1:301585/1‑76 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGGGCTTTTCTAGCCAGTATTGGTCAGCAGTTAGGTACg > 2:1072767/1‑76 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg > 2:318685/1‑76 (MQ=255) tgggAAGCGGTAACGGGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACgg < 1:1705988/73‑1 (MQ=255) ggCGAAGCGGTACCGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACgg < 1:562861/75‑1 (MQ=255) ggAAGGGGTAAGGCGTAAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt < 1:2112254/75‑1 (MQ=255) cGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt > 2:3153490/1‑76 (MQ=255) gAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTg > 1:1472977/1‑76 (MQ=255) aggCGGTAACGGGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 1:4579054/74‑1 (MQ=255) aaGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGATGa > 1:4265722/1‑76 (MQ=255) gCGGTAACGGGGGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAAt < 2:884604/76‑1 (MQ=255) gCGGTAACGCGTGAGGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAAt < 1:1428593/76‑1 (MQ=255) cGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATc > 1:5183071/1‑76 (MQ=255) ggTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATcc > 1:4877743/1‑76 (MQ=255) gTAACGCGTGAAGGTGCTGGCTGGGATAGCTATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATccc < 2:1877898/76‑1 (MQ=255) aaCGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGGTAGGAACGGCTGAATCCCtt > 1:2225272/1‑76 (MQ=255) aaCGCGTGAAGGTGCTGGCTGGGAAAGCGATTTTCTAGCCAGTATTGAGCAGCAGGTAGGAACGGCTGCAACCCtt > 2:1453625/1‑76 (MQ=255) aCGCGTAAAGGTGCGGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTg < 1:3539032/76‑1 (MQ=255) cgggTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGa < 1:4451713/73‑1 (MQ=255) tGAGGGTGCTGGCTGGGATGGCTATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTg < 1:1434364/76‑1 (MQ=255) tGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTTGACTg > 2:2768572/1‑76 (MQ=255) tGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTTAACTg > 1:1041742/1‑76 (MQ=255) gAAGGTGCGGGCTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgg < 1:1039504/76‑1 (MQ=255) aGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTTAACTgggg > 2:5075516/1‑76 (MQ=255) gggctgggtgGGATGGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcg < 2:359005/74‑1 (MQ=255) gTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcg < 2:1637465/76‑1 (MQ=255) tGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggagg < 2:4909924/76‑1 (MQ=255) ctggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcgggt > 2:4599240/1‑74 (MQ=255) tggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTg > 1:4397919/1‑76 (MQ=255) ggcgggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:5096434/76‑1 (MQ=255) gtggggATGCCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGc < 2:4633795/73‑1 (MQ=255) ctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCg > 1:618473/1‑76 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGa > 2:4631174/1‑76 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCgg > 2:3040716/1‑75 (MQ=255) gATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGaaag > 2:1070787/1‑74 (MQ=255) gATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGGAtg > 1:3251196/1‑76 (MQ=255) gCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGAAagtgga > 2:3331319/1‑74 (MQ=255) tgtttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAAt < 1:4303845/73‑1 (MQ=255) gtttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc < 2:3419949/74‑1 (MQ=255) tttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATcc < 2:3997300/75‑1 (MQ=255) aTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGCACTGGGGCGGCCGGCCAAAGTGAGTcc > 2:4623425/1‑76 (MQ=255) ttttTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCTGc < 2:3259917/76‑1 (MQ=255) tCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCt < 1:1231381/76‑1 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGAATGTGAATACGCCTg > 1:2851262/1‑76 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGAATGTGAAATCGcccg > 1:4538146/1‑74 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCCGGCGAACGTCAATCcccccg > 2:3402595/1‑74 (MQ=255) | AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |