| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,485,450 | 0 | A | G | 34.3% | 238.7 / 91.7 | 137 | G193G (GGA→GGG) | ydcV | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/90); new base G (47/0); total (47/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.67e-38 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 160 total aligned reads displayed.
ATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTA > REL606/1485377‑1485525 | aTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggggg > 2:4527707/1‑76 (MQ=255)aTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGCTCGGCGTTACTGGCaggggg > 2:4661221/1‑76 (MQ=255) tCTTGGGGCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAggagga < 1:439198/76‑1 (MQ=255) ctgggggCAAGTGGCGGGAAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGaa < 1:719810/73‑1 (MQ=255) cTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGaa < 2:3813704/76‑1 (MQ=255) ttGGGGCCAATGGCTGACAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggggggaa > 1:1334921/1‑73 (MQ=255) tgggggCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAt < 1:3771725/74‑1 (MQ=255) tgtggCCAAGGCCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑g < 2:2543808/73‑1 (MQ=255) tGGGGCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑g < 2:4452886/76‑1 (MQ=255) tGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑g < 2:1584907/76‑1 (MQ=255) tGGGCCAAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑g < 2:2663224/76‑1 (MQ=255) gCCAATGCCTGGCAATCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTgg < 1:4878184/76‑1 (MQ=255) cAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCg < 2:297262/76‑1 (MQ=255) cAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCg < 1:4704672/76‑1 (MQ=255) aaTGCCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGt < 1:1643184/76‑1 (MQ=255) aTGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGtt < 2:593472/76‑1 (MQ=255) tggctggcAAATCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAA‑GCAGGCGctt > 1:1389560/1‑76 (MQ=255) tgcctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGttt < 2:3165504/73‑1 (MQ=255) tgctggaAAGCCTTCCGCTACGTATTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTg < 2:264092/75‑1 (MQ=255) ggctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GGTGGGGGTTg > 2:1706330/1‑76 (MQ=255) gctggcAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGc < 1:1428311/76‑1 (MQ=255) ggcAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCtt < 1:2702362/76‑1 (MQ=255) ggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCtt < 2:2313745/76‑1 (MQ=255) ggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCtt < 2:724795/76‑1 (MQ=255) cAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAG‑GCCGGGGTGTGCCtttt > 1:875143/1‑74 (MQ=255) aacccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTc < 1:25552/73‑1 (MQ=255) aaCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAA‑GCTGGCGTTTGGCTTGttg > 2:3259666/1‑74 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGATGGCGGGGGTTTG‑CTTGTCGtt > 2:1099187/1‑76 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GGCGGGGGTTTCCTTGTCGtt > 1:623994/1‑76 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAA‑GCTGGCGGTTGCCTTTTCGtt > 2:4323431/1‑76 (MQ=255) cTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GGTGGCGTTTGCCTCTTCGttg > 1:2208255/1‑75 (MQ=255) cTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTc < 1:2079610/76‑1 (MQ=255) tCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAA‑TATGGCGGTTGCCCTGTCGCTCgt > 1:1087335/1‑75 (MQ=255) tCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGa < 2:2374286/76‑1 (MQ=255) cGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGGT‑GCTGGGGGTTGCCTTGTCGTTCGATg > 1:4328335/1‑76 (MQ=255) cGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATg < 2:1607817/76‑1 (MQ=255) cGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATg < 2:1676669/76‑1 (MQ=255) cGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATg < 1:86680/76‑1 (MQ=255) cGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATg < 2:2926306/76‑1 (MQ=255) gCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGa < 1:1016970/76‑1 (MQ=255) gCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGa < 2:4487418/76‑1 (MQ=255) cTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAG‑GGGGGGGGTTTCCTTGTCGTTTTATGaa > 1:2368749/1‑76 (MQ=255) cTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGaa < 1:2907689/76‑1 (MQ=255) tACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GGTGGCGGTTGCCTTGTCGTTTGATGaaa > 1:1040386/1‑76 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GGTGGCGTTTTCCCTGTCGTTTGGTGAAat > 2:1890903/1‑76 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGCGGTTTTCCTGTCGTTTGGTGAAat > 1:487075/1‑76 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGCGGTTGCCCTGTCGTTTGGTGAAat > 1:1571325/1‑76 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAG‑GCTGGCGGTTGCCTTGTTCTTTGATGgaaa > 2:2958248/1‑75 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAat < 1:2549822/76‑1 (MQ=255) cGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTTGCGTTTGCCTTTTCGTTGTCTGAAatc > 2:1452816/1‑76 (MQ=255) cGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAA‑GCTGGCGTTTTGCTTGTCGTTTGATGcaaaa > 1:2332437/1‑74 (MQ=255) cGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAatc < 2:5074112/76‑1 (MQ=255) cGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAatc < 2:4293273/76‑1 (MQ=255) cGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAatc < 1:4560617/76‑1 (MQ=255) ttAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAatca < 1:633308/75‑1 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGGGGTTGCCTTGTCGTTCGCTGAAataa > 2:854467/1‑74 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAatca < 1:2742423/76‑1 (MQ=255) tAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGCGTTTTCCTTGTCGTTCGATGAAatcat > 1:3666698/1‑76 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGCGTTTGCCTTTTCGTTCGATGAAaacatc > 1:957294/1‑76 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAatcatc < 1:404305/76‑1 (MQ=255) tgtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGGGTTTGCCCTGTCGTTCGATGAAAATATCGt > 1:4689041/1‑76 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GGTGGCGTTTTCCCTTTCGTTCGGTGAAaacatctct > 1:1789753/1‑73 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGtt < 1:4527707/76‑1 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGtt < 1:765704/76‑1 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAAACATCGTTAc > 1:2254684/1‑76 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAG‑GCTGGCGTTTGCCTTGTTGTTCGCTGAAAACATCGTTAc > 2:1873381/1‑76 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAA‑GGTGGGGGTTGGCTTGTCGTTTGCTGAAATAATCGTTAc > 2:3901157/1‑76 (MQ=255) gCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTAca < 2:5048325/76‑2 (MQ=255) gCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACg < 1:3881169/76‑1 (MQ=255) gCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACg < 1:2954614/76‑1 (MQ=255) ccGAATCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGCGTTTGTCTTGTTGTTCGATGAAATCATCGTTTCGa > 1:2830778/1‑76 (MQ=255) cGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGAc < 2:3705965/76‑1 (MQ=255) gAATCCCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGAcc < 1:4939771/76‑1 (MQ=255) aaTCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGCGGTTGGCTTGTCGTTCGCTGAAATCATCGTTTCGACCt > 2:4189249/1‑76 (MQ=255) aaTCTCAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGCGGTTGCCTTGTCGTTCGATGAAAACATCATTACGACCt > 2:240957/1‑76 (MQ=255) aaTCTCAGTTCGGCGTTACTGGCAGGGGGGAA‑GGTGGGGGTTTTCTTGTCGTTCGGTGAAATAATCGTTACGACCt > 2:3403530/1‑76 (MQ=255) aaTCTCAGTTCGGCGTTACTGGCAGGGGGAAA‑TCTGGGGGGTTCCTTCGCGGTCGATGAAATCAATGGTACGATCt > 2:4552720/1‑76 (MQ=255) aaTCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCt < 1:4853311/76‑1 (MQ=255) tctcAGTTCGGCGTTACTGGCAGGGGGGAT‑GGTGGGGGTTTGCTTGTTGTTCGATGAAAACCTCGTTACGACCttt > 2:4598355/1‑76 (MQ=255) ctcAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTa < 2:1185575/76‑1 (MQ=255) cAGTTCGGCGTTACTGGCAGGGGGGAT‑GCTGGGGTTTGCCTTGTCGTTCGCTGAAATCATCGTTTCGACCTTTACg > 1:1011175/1‑76 (MQ=255) gtgcGGCGTTACTGGCCGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGc < 1:5157229/73‑1 (MQ=255) gTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCTTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGc < 2:122323/76‑1 (MQ=255) ggCGTTACTGGCAGGGGGGAA‑GCTGGCGGTTTCCTTGTAGTTCGGTGAAAACATCGTTACGACCTTTTCGGGAggg > 2:631621/1‑75 (MQ=255) gCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTc < 1:2299092/76‑1 (MQ=255) gTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCAt < 1:4552490/76‑1 (MQ=255) gTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCAt < 2:3277300/76‑1 (MQ=255) aCTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaa < 1:654932/76‑1 (MQ=255) cTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaac < 1:4493307/76‑1 (MQ=255) gCAGGAGGAAT‑GCT‑GCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac < 1:4023363/76‑1 (MQ=255) cAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac < 2:767159/76‑1 (MQ=255) cAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac < 2:2880853/76‑1 (MQ=255) cAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac < 1:5058608/76‑1 (MQ=255) cAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac < 1:97798/76‑1 (MQ=255) cAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac < 1:1960163/76‑1 (MQ=255) cAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac < 2:3025253/76‑1 (MQ=255) aggaggAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGaacgaacg < 2:4974410/76‑1 (MQ=255) aggaggAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGaacgaacg < 1:4179254/76‑1 (MQ=255) aggaggAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTGACGACCTGTACGGCAGGGCGTggacgacac > 2:2774340/1‑73 (MQ=255) aggaAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTa < 1:3779331/76‑1 (MQ=255) | ATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAT‑GCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTA > REL606/1485377‑1485525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |