BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	474,629	A→G	L226P (CTG→CCG)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	474,629	0	A	G	100.0%	392.3 / NA	96	L226P (CTG→CCG)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (54/42); total (54/42)

Only 100 of 110 total aligned reads displayed.

AAAGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATA  >  REL606/474556‑474700

aaaGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGc                                                                       >  2:2110752/1‑76 (MQ=255)

aaaGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGc                                                                       >  2:1135670/1‑76 (MQ=255)

aaaGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGc                                                                       >  2:290681/1‑76 (MQ=255)

aaaGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGc                                                                       >  2:2810017/1‑76 (MQ=255)

 aaGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCt                                                                      >  1:3679952/1‑76 (MQ=255)

 aaGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGCAAACGGCt                                                                      >  1:4947240/1‑76 (MQ=255)

   ggAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTcg                                                                    <  1:4533130/76‑1 (MQ=255)

   ggAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTcg                                                                    <  1:4420191/76‑1 (MQ=255)

     agaCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTcgcg                                                                  >  1:513841/1‑76 (MQ=255)

     agaCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTcgcg                                                                  >  1:4798758/1‑76 (MQ=255)

       aCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCgag                                                                >  2:2153046/1‑76 (MQ=255)

       aCATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCgag                                                                >  2:418089/1‑76 (MQ=255)

        cATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCgaga                                                               <  2:3074156/76‑1 (MQ=255)

        cATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCgaga                                                               >  1:545780/1‑76 (MQ=255)

          tCAAACAGCTCTACCGCTCCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAAc                                                             >  2:1022732/1‑76 (MQ=255)

          tCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAAc                                                             <  1:1600495/76‑1 (MQ=255)

           cAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAAcc                                                            >  2:1993661/1‑76 (MQ=255)

           cAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAAcc                                                            >  2:1437147/1‑76 (MQ=255)

            aaaCAGCTCTCCCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCg                                                           <  2:483097/76‑1 (MQ=255)

            aaaCAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCg                                                           >  1:4147818/1‑76 (MQ=255)

            aaaCAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCg                                                           >  2:2861637/1‑76 (MQ=255)

             aaCAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGCGAACCgg                                                          >  2:1440219/1‑76 (MQ=255)

             aaCAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCgg                                                          >  2:2407657/1‑76 (MQ=255)

             aaCAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCgg                                                          <  1:2548731/76‑1 (MQ=255)

             aaCAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCgg                                                          <  1:1762628/76‑1 (MQ=255)

              aCAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGt                                                         <  1:223402/76‑1 (MQ=255)

              aCAGCGCTACCGCACAAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGt                                                         <  2:1503828/76‑1 (MQ=255)

                aGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTg                                                       <  2:2395566/76‑1 (MQ=255)

                aGCCCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTg                                                       <  1:3912468/76‑1 (MQ=255)

                 gCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCTAGCACCGACAGGGTAAACGGCTCGCGAGAAGCGGTTGc                                                      >  2:4711243/1‑76 (MQ=255)

                 gCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGtgcc                                                      >  2:2671367/1‑73 (MQ=255)

                  ctctACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGGTGcc                                                     >  2:2208082/1‑76 (MQ=255)

                   tctACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTACACGGCACGCGAGAACCGGGTGCcg                                                    >  2:1231120/1‑76 (MQ=255)

                   tctACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCcg                                                    >  2:1701352/1‑76 (MQ=255)

                   tctACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCcg                                                    >  2:1676000/1‑76 (MQ=255)

                    ctACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGGTGcccc                                                   >  1:579953/1‑74 (MQ=255)

                     tACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCcgcg                                                  <  1:58670/76‑1 (MQ=255)

                      aCCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGGACCGGTTGCcgccc                                                 >  1:1594984/1‑74 (MQ=255)

                        cGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTg                                               <  2:1450239/76‑1 (MQ=255)

                         gCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGc                                              <  2:3067262/76‑1 (MQ=255)

                          gcccAAAGGCACCCCCTACCGCCAGCGCCAGCGCCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCg                                             <  2:2175598/74‑1 (MQ=255)

                           aCCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGt                                            <  1:521191/76‑1 (MQ=255)

                             cAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGc                                          >  2:3894293/1‑76 (MQ=255)

                             cAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGGTGCCGCGCCGCGTGc                                          >  1:2111392/1‑76 (MQ=255)

                              aaaGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTTCGTGcc                                         >  2:2888975/1‑76 (MQ=255)

                              aaaGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCCCGAGAACCGGGTGCCGCGCCGCGTGcc                                         >  1:257179/1‑76 (MQ=255)

                                aGGCAGCCCCTAACGCCAGCGCCAGCACCGACAGGTTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCAt                                       <  1:512592/76‑1 (MQ=255)

                                aGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCAt                                       <  1:2445505/76‑1 (MQ=255)

                                 ggCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGAGcccca                                      >  2:2990425/1‑73 (MQ=255)

                                 gccAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATa                                      <  2:2887472/74‑1 (MQ=255)

                                   cAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAAt                                    <  2:5068203/76‑1 (MQ=255)

                                    aaCCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATc                                   <  2:459210/76‑1 (MQ=255)

                                     aCCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATcc                                  <  1:3517209/76‑1 (MQ=255)

                                       cccTACCGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCAc                                <  1:1729862/76‑1 (MQ=255)

                                        ccTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACg                               >  1:5013652/1‑76 (MQ=255)

                                         cTAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCGGCGTGCCATAATCCACgg                              >  1:2558146/1‑76 (MQ=255)

                                          tAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGc                             <  1:2923807/76‑1 (MQ=255)

                                          tAACGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGGGCCATAATCCACGGc                             >  1:4740494/1‑76 (MQ=255)

                                           agcgccagcgcaagCACCGACAGGTTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCa                            <  1:1231120/74‑1 (MQ=255)

                                           aaCGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGcc                            >  2:3367787/1‑75 (MQ=255)

                                           aaCGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCa                            >  2:135627/1‑76 (MQ=255)

                                           aaCGCCAGCGCCAGCACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCa                            <  1:1701352/76‑1 (MQ=255)

                                              gccagcgccagcACCGACAGGGTAAACGGCTCGCGAGAACCGGGTGCCGCGCTGCGGGCCATAATCCACGGCAccc                         >  2:821485/1‑75 (MQ=255)

                                                cagcgccagcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAgc                       >  2:1080304/1‑75 (MQ=255)

                                                 agcgccagcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGAc                      <  1:4834037/76‑1 (MQ=255)

                                                 agcgccagcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCCCGCTGCGTGCCATAATCCACGGCACCAgcc                      >  1:172704/1‑74 (MQ=255)

                                                  gcgccagcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACg                     <  2:2947545/76‑1 (MQ=255)

                                                   cgccagcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACgg                    <  1:314393/76‑1 (MQ=255)

                                                    gccggcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGc                   <  1:231031/76‑1 (MQ=255)

                                                    gccagcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGc                   <  2:4878614/76‑1 (MQ=255)

                                                    gccagcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGc                   >  1:3389617/1‑76 (MQ=255)

                                                    gccagcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGc                   <  1:454588/76‑1 (MQ=255)

                                                      cagcACCGACAGGGTAAACGGCTCGCGAGAACCGGGTGGCCCGCTGCGTGCCCTAATCCACCGCACCAGACGGCGa                 >  2:157785/1‑76 (MQ=255)

                                                       agcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGAc                <  2:2426576/76‑1 (MQ=255)

                                                       agcACCGACAGGGTAAACGGCTCGCGAGAAACGGGTGCCGCGCTCCGTGCCATAATCCACGGCACCCGACAGGGAc                >  2:3040898/1‑76 (MQ=255)

                                                        gcACCGCCAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGAcc               <  2:1777430/76‑1 (MQ=255)

                                                        gcACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCCCCAGACGGCGAcc               <  2:4888193/76‑1 (MQ=255)

                                                         ccccGACAGGGTAAACGGCTCGCGAGAACCGTTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCt              <  2:4456517/74‑1 (MQ=255)

                                                         cACCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCt              <  2:665174/76‑1 (MQ=255)

                                                          aCCGACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTa             <  1:1254306/76‑1 (MQ=255)

                                                           ccGACAGGGTAACCGGCTCGCAAGAACCGGTTGCCGCGCTGCGTGCCATAACCCACGGCCCCAGACGGCGACCTAc            <  2:4097036/76‑1 (MQ=255)

                                                             gACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCCGACGGCGACCTACCa          >  1:1500268/1‑76 (MQ=255)

                                                             gACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCCGACGGCGACCTACCa          >  1:1792634/1‑76 (MQ=255)

                                                             gACAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCa          <  2:1072608/76‑1 (MQ=255)

                                                              gcAGGGTAAGCGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAg         <  2:866835/75‑1 (MQ=255)

                                                              aCAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAg         <  2:4912172/76‑1 (MQ=255)

                                                              aCAGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAg         <  2:4039955/76‑1 (MQ=255)

                                                                aGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCCGca       >  1:5060150/1‑76 (MQ=255)

                                                                aGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGca       >  1:307675/1‑76 (MQ=255)

                                                                aGGGTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGca       >  1:2622316/1‑76 (MQ=255)

                                                                  gttAAACGGCTCGCGAGACCCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGcatc     <  2:4865490/74‑1 (MQ=255)

                                                                  ggcaaaCGGCTGGCGAGACCCGGTTGCCGCCCTGGGTGCCATACTCCCCGGCCCCAGCCGGCGACCTACCAGcatc     <  2:4947240/73‑1 (MQ=255)

                                                                  ggTAAGCGGCTCGCGAGAACCGGTTTCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGcatc     <  2:3679952/76‑1 (MQ=255)

                                                                   gTAACCGGCTCGCGAGACCCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGcatca    <  1:602672/76‑1 (MQ=255)

                                                                   gTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCCTAATCCACGGCACCAGACGGCGACCTACCAGcatca    >  2:4808202/1‑76 (MQ=255)

                                                                   gTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGcatca    <  1:2644688/76‑1 (MQ=255)

                                                                   gTAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCACcatca    >  2:687968/1‑76 (MQ=255)

                                                                    tAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGGCCTCCCAGcatcat   >  1:894448/1‑76 (MQ=255)

                                                                    tAAACGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGcatcat   >  1:3581675/1‑76 (MQ=255)

                                                                     aaaCGGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACCGCGACCTACCCGCATCATa  >  1:2616240/1‑76 (MQ=255)

AAAGGAGACATCAAACAGCTCTACCGCACCAAAGGCAACCCCTAACGCCAGCGCCAGCACCGACAGGGTAAACAGCTCGCGAGAACCGGTTGCCGCGCTGCGTGCCATAATCCACGGCACCAGACGGCGACCTACCAGCATCATA  >  REL606/474556‑474700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`