| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,145,750 | C→T | intergenic (+81/‑114) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,145,750 | 0 | C | T | 98.8% | 630.1 / ‑1.9 | 164 | intergenic (+81/‑114) | dnaG/rpoD | DNA primase/RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (92/70); total (92/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01 | |||||||||||
Only 100 of 166 total aligned reads displayed.
CTTAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATC > REL606/3145678‑3145822 | cTTAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCt > 2:2301175/1‑76 (MQ=255)cTTAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCt > 1:2313211/1‑76 (MQ=255)cTTAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCt > 1:1742805/1‑76 (MQ=255) ttAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTc > 2:1258157/1‑76 (MQ=255) ttAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTc > 1:2205062/1‑76 (MQ=255) tAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCg > 2:4080953/1‑76 (MQ=255) tAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCg > 2:1391440/1‑76 (MQ=255) aaGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGt > 2:1834209/1‑76 (MQ=255) aaGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGt > 2:513685/1‑76 (MQ=255) aaGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGt > 2:238528/1‑76 (MQ=255) tGCCGAAGAGCGAGCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAAt < 1:2259514/76‑1 (MQ=255) gCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAAtt > 1:4899160/1‑76 (MQ=255) gCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAAtt < 1:4256602/76‑1 (MQ=255) gCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAAtt < 2:3629968/76‑1 (MQ=255) gCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAAtt < 1:3994282/76‑1 (MQ=255) cgcGAAGAGCTATCGGAACCCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAAtt < 2:2594105/74‑1 (MQ=255) cGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTAt > 2:4646941/1‑76 (MQ=255) cGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTAt > 2:2483012/1‑76 (MQ=255) cGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTAt < 1:3762200/76‑1 (MQ=255) gAAGAGCGTCCGGGAGCCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATc < 2:4785074/76‑1 (MQ=255) gAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATc > 2:35700/1‑76 (MQ=255) gAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATc > 1:2795316/1‑76 (MQ=255) aaGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCg > 2:343650/1‑76 (MQ=255) aaGAGCGAGCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCg < 1:4802143/76‑1 (MQ=255) agagCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGt > 2:1738226/1‑76 (MQ=255) agagCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGt > 2:1025306/1‑76 (MQ=255) agagCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGt > 2:2968793/1‑76 (MQ=255) ggCGATGGGAACGCCCCCGCCAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTg < 2:4662626/75‑1 (MQ=255) gATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCg < 1:2170252/76‑1 (MQ=255) cGGGAAGCCCCCGACAGCCGCACTGAGCGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCggga > 1:2827287/1‑74 (MQ=255) cGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTa > 2:1372674/1‑76 (MQ=255) cGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTa > 1:4641182/1‑76 (MQ=255) cGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTa > 2:2610874/1‑76 (MQ=255) cGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTa > 1:4991847/1‑76 (MQ=255) gggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTaa < 2:1116092/76‑1 (MQ=255) gggAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTaa > 1:549469/1‑76 (MQ=255) gAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAaac < 1:2463998/76‑1 (MQ=255) gccccccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAaacaa < 2:4982696/74‑1 (MQ=255) gCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAaacaac < 1:320568/76‑1 (MQ=255) gCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAaacaac > 1:4713595/1‑76 (MQ=255) cccccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAAcc > 2:2561592/1‑76 (MQ=255) cccccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGCAAACAAcc > 2:4051728/1‑76 (MQ=255) ccccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCg < 1:3144125/76‑1 (MQ=255) ccccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCg < 1:2074215/76‑1 (MQ=255) ccccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCg < 1:387806/76‑1 (MQ=255) ccGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGtt < 2:2178759/76‑1 (MQ=255) gccAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTgg < 2:3221863/74‑1 (MQ=255) gACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTgg < 1:2636801/76‑1 (MQ=255) ccAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGa < 1:1667089/75‑1 (MQ=255) aCAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGa > 2:4106783/1‑76 (MQ=255) aCAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGa < 2:1937698/76‑1 (MQ=255) gCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGAttt < 1:844307/76‑1 (MQ=255) gCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGAttt < 2:2779486/76‑1 (MQ=255) ccGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTc < 1:1498032/76‑1 (MQ=255) ccGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTc < 2:3110901/76‑1 (MQ=255) ccGCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTc < 2:195372/76‑1 (MQ=255) gCACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAg > 1:1867703/1‑76 (MQ=255) cACTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGc > 1:2917361/1‑76 (MQ=255) cTGAGAGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGt > 2:410629/1‑76 (MQ=255) gagaGGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTa < 1:3316581/76‑1 (MQ=255) agagGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTaa > 2:3472079/1‑76 (MQ=255) agagGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTaa > 1:655399/1‑76 (MQ=255) gagGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAAc > 2:3233795/1‑76 (MQ=255) gagGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAAc > 1:65042/1‑76 (MQ=255) agGCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACg > 1:1175514/1‑76 (MQ=255) ggCAGCGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACgg < 2:3190982/76‑1 (MQ=255) gggCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGaa < 2:2859578/75‑1 (MQ=255) cGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTgga > 2:4885080/1‑74 (MQ=255) cGGCAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGaa < 2:4776986/76‑1 (MQ=255) cAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGa > 1:2337037/1‑76 (MQ=255) cAAATATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGa > 2:3864514/1‑76 (MQ=255) aaaTATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAgggc > 1:4991472/1‑74 (MQ=255) aaaTATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGAc > 2:4568831/1‑76 (MQ=255) aaaTATATAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGAc < 2:3651302/76‑1 (MQ=255) atatatAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGCTTTCAGCGTTAACGGCTGGAGGAAAt > 1:4326501/1‑76 (MQ=255) atatatAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGc > 1:4598066/1‑57 (MQ=255) atatatAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGc < 2:4598066/57‑1 (MQ=255) atatatAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACAt < 2:208522/76‑1 (MQ=255) atatatAAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACAt > 2:1060921/1‑76 (MQ=255) tatataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACaac > 2:3339900/1‑74 (MQ=255) atataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGGCATCg > 2:1251915/1‑76 (MQ=255) atataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCg > 2:3097319/1‑76 (MQ=255) atataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCg > 1:5120476/1‑76 (MQ=255) atataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCg > 1:3709385/1‑76 (MQ=255) atataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCg > 2:1578699/1‑76 (MQ=255) tataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCgg < 1:238528/76‑1 (MQ=255) tataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCgg < 1:1834209/76‑1 (MQ=255) ataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCggg < 2:2254862/76‑1 (MQ=255) ataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCggg > 1:5122646/1‑76 (MQ=255) ataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCggg < 1:4136115/76‑1 (MQ=255) ataAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAAGGCATCggg > 1:502310/1‑76 (MQ=255) agaaGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCggg < 1:4498824/74‑1 (MQ=255) taAGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTACCGGCTGAAGGACATCgggg > 1:3940270/1‑75 (MQ=255) aaGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCggggc > 2:175476/1‑74 (MQ=255) aaGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTc < 2:649599/76‑1 (MQ=255) aaGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTc < 1:2174733/76‑1 (MQ=255) aGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCa < 1:4285034/76‑1 (MQ=255) aGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCa > 1:1358325/1‑76 (MQ=255) aGTACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGAAAACGGGGCa > 2:3515107/1‑76 (MQ=255) tACGTCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAAt < 2:2851120/76‑1 (MQ=255) | CTTAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACGCCCTCGTAATTATCGTTGGCGGTAAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATC > REL606/3145678‑3145822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |