BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,285,721	A→C	intergenic (‑27/+203)	yhbL ← / ← arcB	isoprenoid biosynthesis protein with amidotransferase‑like domain/hybrid sensory histidine kinase in two‑component regulatory system with ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,285,721	0	A	C	100.0%	687.9 / NA	154	intergenic (‑27/+203)	yhbL/arcB	isoprenoid biosynthesis protein with amidotransferase‑like domain/hybrid sensory histidine kinase in two‑component regulatory system with ArcA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (72/82); total (72/82)

Only 100 of 156 total aligned reads displayed.

CCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGAAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTAAG  >  REL606/3285648‑3285793

ccGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGcaa                                                                        >  1:1648212/1‑76 (MQ=255)

 cGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAac                                                                       <  2:203634/76‑1 (MQ=255)

  gTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAaca                                                                      <  2:1546420/76‑1 (MQ=255)

  gTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAaca                                                                      >  1:4681351/1‑76 (MQ=255)

   tCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATTATCCTTTCGCAATTGACTGCAacac                                                                     <  1:596023/76‑1 (MQ=255)

    cATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAAcaca                                                                    >  1:4474931/1‑76 (MQ=255)

    cATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAAcaca                                                                    >  2:4102346/1‑76 (MQ=255)

     aTAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACAt                                                                   >  1:86153/1‑76 (MQ=255)

     aTAGACGCCGCATACGCTCAGAATTACGCTAATTTTCTTCATTGTGATCATCCTTTCGCAATTGCCTGCAACACAt                                                                   <  2:2072785/76‑1 (MQ=255)

      tAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACAtt                                                                  <  2:3173330/76‑1 (MQ=255)

      tAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACAtt                                                                  >  1:376168/1‑76 (MQ=255)

       aGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTa                                                                 >  1:263103/1‑76 (MQ=255)

       aGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTa                                                                 <  1:2677513/76‑1 (MQ=255)

       aGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTa                                                                 <  1:2824903/76‑1 (MQ=255)

         aCGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAAt                                                               <  1:4117825/76‑1 (MQ=255)

         aCGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAAt                                                               <  2:433027/76‑1 (MQ=255)

         aCGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAAt                                                               <  2:4788352/76‑1 (MQ=255)

          cgccgcATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAAtt                                                              <  1:1078950/76‑1 (MQ=255)

            ccgcATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAAtttt                                                            <  1:5189104/76‑1 (MQ=255)

              gcATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAAtttttt                                                          <  2:36289/76‑1 (MQ=255)

                aTCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTta                                                        <  1:2496231/76‑1 (MQ=255)

                aTCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTta                                                        <  2:1735347/76‑1 (MQ=255)

                aTCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTta                                                        <  1:4052081/76‑1 (MQ=255)

                aTCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTta                                                        >  1:4064726/1‑76 (MQ=255)

                  ccGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAGTTGACTGCAACACATTAATTTTTTtaat                                                      >  1:2435469/1‑76 (MQ=255)

                  ccGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTtaat                                                      <  2:1270271/76‑1 (MQ=255)

                   cGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTtaata                                                     <  1:1069800/76‑1 (MQ=255)

                     cccAGAAGTACGACGATTTTCTTCATTGTGATCATCCTTTCGCAAGTGACTGCAACACATTAATTTTTTGAATaaa                                                   <  2:136181/74‑1 (MQ=255)

                     cTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATaaa                                                   <  1:3845317/76‑1 (MQ=255)

                     cTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATaaa                                                   <  2:43213/76‑1 (MQ=255)

                     cTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATaaa                                                   <  2:136182/76‑1 (MQ=255)

                      tCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATaaaa                                                  <  2:1136634/76‑1 (MQ=255)

                      tCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATaaaa                                                  >  2:4049188/1‑76 (MQ=255)

                       cAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATaaaaa                                                 >  2:159395/1‑76 (MQ=255)

                       cAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATaaaaa                                                 >  2:1555047/1‑76 (MQ=255)

                        aGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAAt                                                <  2:2802719/76‑1 (MQ=255)

                        aGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAAt                                                <  1:2092630/76‑1 (MQ=255)

                        aGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAAt                                                >  2:3919477/1‑76 (MQ=255)

                        aGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATCAATTTTTTTAATAAAAAt                                                <  1:3467797/76‑1 (MQ=255)

                         gAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATg                                               <  2:2483553/76‑1 (MQ=255)

                          aaTTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATgg                                              >  1:3145044/1‑76 (MQ=255)

                          aaTTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATgg                                              >  2:3073326/1‑76 (MQ=255)

                           aTTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGt                                             <  2:788686/76‑1 (MQ=255)

                           aTTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGt                                             <  1:2153855/76‑1 (MQ=255)

                           aTTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGt                                             <  1:1142632/76‑1 (MQ=255)

                           aTTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGt                                             >  1:3742511/1‑76 (MQ=255)

                             tACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTAc                                           <  2:3111532/76‑1 (MQ=255)

                              aCGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACg                                          <  1:384732/76‑1 (MQ=255)

                               cGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGc                                         <  2:3640087/76‑1 (MQ=255)

                                  cAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATc                                      >  2:1714727/1‑76 (MQ=255)

                                     ttttCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATcaca                                   <  2:2969575/76‑1 (MQ=255)

                                     ttttCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATcaca                                   >  1:2927867/1‑76 (MQ=255)

                                      tttCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATcacac                                  <  2:3175292/76‑1 (MQ=255)

                                      tttCTTCATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATcacac                                  >  2:2724574/1‑76 (MQ=255)

                                       ttcttcATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATcacaca                                 >  1:2883912/1‑76 (MQ=255)

                                         cttcATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACAtt                               >  1:1923921/1‑76 (MQ=255)

                                         cttcATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACAtt                               >  1:5060985/1‑76 (MQ=255)

                                          ttcATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACAttt                              <  2:3800248/76‑1 (MQ=255)

                                          ttcATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACAttt                              <  2:1651249/76‑1 (MQ=255)

                                           tcATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTa                             >  2:5040768/1‑76 (MQ=255)

                                            cATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTaa                            <  2:4898727/76‑1 (MQ=255)

                                            cATTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTaa                            >  2:3408100/1‑76 (MQ=255)

                                             aTTGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAAc                           >  1:1764300/1‑76 (MQ=255)

                                              ttGTGATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACt                          >  1:937239/1‑76 (MQ=255)

                                               tgtgATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTg                         <  1:3571290/76‑1 (MQ=255)

                                               tgtgATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTg                         >  1:3333057/1‑76 (MQ=255)

                                                 tgATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGAt                       >  1:3187098/1‑76 (MQ=255)

                                                  gATCATCCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGAtt                      >  1:1477129/1‑76 (MQ=255)

                                                    tcatcCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCa                    <  2:4066794/76‑1 (MQ=255)

                                                     catcCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCAt                   >  2:3278199/1‑76 (MQ=255)

                                                     catcCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCAt                   >  2:3961608/1‑76 (MQ=255)

                                                      atcCTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATg                  <  2:2064993/76‑1 (MQ=255)

                                                        ccTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATTTa                <  2:3761696/76‑1 (MQ=255)

                                                        ccTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTa                <  1:5011474/76‑1 (MQ=255)

                                                        ccTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTa                >  1:443965/1‑76 (MQ=255)

                                                        ccTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTa                <  1:1699312/76‑1 (MQ=255)

                                                        ccTTTCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTa                >  1:233661/1‑76 (MQ=255)

                                                          tttCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAAc              <  1:2451706/76‑1 (MQ=255)

                                                           ttCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACa             >  1:4731312/1‑76 (MQ=255)

                                                           ttCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACa             >  1:1384738/1‑76 (MQ=255)

                                                           ttCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACa             <  1:3528058/76‑1 (MQ=255)

                                                           ttCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACa             <  1:179361/76‑1 (MQ=255)

                                                            tCGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGtaataa            <  1:4036985/76‑1 (MQ=255)

                                                             cGCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACaaa           >  1:1248591/1‑76 (MQ=255)

                                                              gCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAAt          >  1:4587456/1‑76 (MQ=255)

                                                              gCAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAAt          >  1:2742137/1‑76 (MQ=255)

                                                               cAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATc         >  1:833141/1‑76 (MQ=255)

                                                               cAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATc         >  1:1533136/1‑76 (MQ=255)

                                                               cAATTGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATc         >  1:4032216/1‑76 (MQ=255)

                                                                  ttGACTGCAACACATCAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCAtt      >  2:5124300/1‑76 (MQ=255)

                                                                   tGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCAttt     <  2:4378329/76‑1 (MQ=255)

                                                                   tGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCAttt     >  1:3024268/1‑76 (MQ=255)

                                                                   tGACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCAttt     <  1:132266/76‑1 (MQ=255)

                                                                    gACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTa    <  1:1898552/76‑1 (MQ=255)

                                                                    gACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTa    <  1:2522773/76‑1 (MQ=255)

                                                                    gACTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTa    <  2:2263724/76‑1 (MQ=255)

                                                                     aCTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTaa   <  1:1714727/76‑1 (MQ=255)

                                                                     aCTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTaa   <  2:613340/76‑1 (MQ=255)

                                                                      cTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTAAg  >  1:2797412/1‑76 (MQ=255)

                                                                      cTGCAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTAAg  >  2:892334/1‑76 (MQ=255)

CCGTCATAGACGCCGCATCCGCTCAGAATTACGCCAATTTTCTTCATTGTGATCATCCTTTCGCAATTGACTGAAACACATTAATTTTTTTAATAAAAATGGTACGCATCACACATTTAACTGATTCATGTAACAAATCATTTAAG  >  REL606/3285648‑3285793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`