| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,341,748 | A→G | intergenic (‑392/‑7) | envR ← / → acrE | DNA‑binding transcriptional regulator/cytoplasmic membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,341,748 | 0 | A | G | 97.6% | 702.6 / 3.1 | 168 | intergenic (‑392/‑7) | envR/acrE | DNA‑binding transcriptional regulator/cytoplasmic membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (1/3); new base G (86/78); total (87/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
Only 100 of 170 total aligned reads displayed.
AAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCC > REL606/3341675‑3341820 | aaaCTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGAt > 2:3265582/1‑76 (MQ=255)aaaCTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGAt < 1:3764952/76‑1 (MQ=255) aaCTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATa < 2:940200/76‑1 (MQ=255) aaCTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATa < 2:779505/76‑1 (MQ=255) aaCTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATa > 2:1947164/1‑76 (MQ=255) aCTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAg < 2:3783734/76‑1 (MQ=255) cTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGt < 2:3707277/76‑1 (MQ=255) tGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTa < 1:223180/76‑1 (MQ=255) tATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAAt < 1:1099596/76‑1 (MQ=255) tATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAAt < 1:300810/76‑1 (MQ=255) aTTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATg > 2:3759395/1‑76 (MQ=255) aTTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATg < 1:2710630/76‑1 (MQ=255) aTTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATg < 1:5171638/76‑1 (MQ=255) tttttCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGa < 2:271790/76‑1 (MQ=255) ttttCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGAc < 2:3173692/76‑1 (MQ=255) ttttCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGAc < 1:4685343/76‑1 (MQ=255) tttctttcTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACg > 2:4302973/1‑76 (MQ=255) tttctttcTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACg < 2:1301676/76‑1 (MQ=255) tttctttcTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACg < 1:540233/76‑1 (MQ=255) tttctttcTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACg > 1:2838688/1‑76 (MQ=255) tttctttcTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACg > 2:2483379/1‑76 (MQ=255) tttctttcTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACg > 1:547418/1‑76 (MQ=255) tctttctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGaa > 2:1272737/1‑76 (MQ=255) tctttctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGaa < 2:1605738/76‑1 (MQ=255) tctttctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGaa < 1:1687040/76‑1 (MQ=255) ctttctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGaaa > 1:1413155/1‑76 (MQ=255) ctttctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGaaa < 1:3066540/76‑1 (MQ=255) ctttctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGaaa > 1:803293/1‑76 (MQ=255) ctttctGCGAGTTAACGAGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGaaa > 1:767284/1‑76 (MQ=255) tttctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAAc < 2:4466576/76‑1 (MQ=255) tctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACAt > 2:628166/1‑76 (MQ=255) tctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACAt < 2:3587686/76‑1 (MQ=255) tctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACAt > 1:560654/1‑76 (MQ=255) ctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATg < 2:2599623/76‑1 (MQ=255) ctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATg > 1:2829840/1‑76 (MQ=255) ctGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATg > 2:500288/1‑76 (MQ=255) tGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGc < 1:2958951/76‑1 (MQ=255) gCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGcc < 2:1413155/76‑1 (MQ=255) gCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGcc > 1:4451748/1‑76 (MQ=255) cGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCa > 1:2425857/1‑76 (MQ=255) cGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCa > 2:4085240/1‑76 (MQ=255) gAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAg < 1:2222457/76‑1 (MQ=255) aGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAgg < 1:5048133/76‑1 (MQ=255) gTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGt > 1:2322021/1‑76 (MQ=255) gTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGt > 1:4840773/1‑76 (MQ=255) ttAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGtt < 2:1026381/76‑1 (MQ=255) ttAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGtt < 1:1571394/76‑1 (MQ=255) aaCGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGtttt > 1:1944040/1‑76 (MQ=255) aaCGAGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGtttt > 1:1590828/1‑76 (MQ=255) cgcgTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTtc > 1:2179196/1‑76 (MQ=255) cgTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTtcct < 2:3290758/76‑1 (MQ=255) cgTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTtcct > 1:322755/1‑76 (MQ=255) tGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTcctcct > 2:2954859/1‑76 (MQ=255) gCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTg < 1:1057527/76‑1 (MQ=255) gCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTg < 2:2554419/76‑1 (MQ=255) tttCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGccc > 1:4033775/1‑76 (MQ=255) tttCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGccc > 1:4244911/1‑76 (MQ=255) tttCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGccc < 2:67494/76‑1 (MQ=255) ttCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCcct > 2:1127968/1‑76 (MQ=255) cTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCcctcc > 2:436047/1‑76 (MQ=255) cTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCcctcc < 2:1773831/76‑1 (MQ=255) tGGGTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCcctcct > 2:4404532/1‑76 (MQ=255) gggTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCtt > 1:1541758/1‑76 (MQ=255) gggTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCtt > 2:499970/1‑76 (MQ=255) ggTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCttt < 1:1830042/76‑1 (MQ=255) ggTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCttt < 1:512302/76‑1 (MQ=255) ggTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCttt > 2:1161438/1‑76 (MQ=255) ggTAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCttt > 2:1604748/1‑76 (MQ=255) tAAATAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTAt > 2:2461574/1‑76 (MQ=255) aaaTAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTAtt > 1:4789688/1‑76 (MQ=255) aaaTAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTAtt < 1:2339326/76‑1 (MQ=255) aaaTAACGCGCTTTCGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTAtt < 2:4819691/76‑1 (MQ=255) tAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTg < 1:3167738/76‑1 (MQ=255) tAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTg < 1:1524325/76‑1 (MQ=255) tAACGCGCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTg < 2:2791705/76‑1 (MQ=255) cgcgCTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGAtc > 2:3898991/1‑76 (MQ=255) cgcTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGAtctc > 1:1314605/1‑76 (MQ=255) cgcTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGAtctc > 1:1899786/1‑76 (MQ=255) cgcTTTTGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGAtctc > 1:4678625/1‑76 (MQ=255) tttGGTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCcgcg > 2:561322/1‑76 (MQ=255) ttGGTTATTTGAGGGATAGTAATGACGAAACAAGCCAGGTTTTTCCTCCTGCACTCCTTTATTCTGATCTCCGCgg > 2:1297657/1‑76 (MQ=255) ggTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCt > 1:656617/1‑76 (MQ=255) ggTTTTTTGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCt < 2:3148561/76‑1 (MQ=255) tttttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTa < 2:2170812/76‑1 (MQ=255) tttttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTa < 2:2829840/76‑1 (MQ=255) ttttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTaa < 2:3767796/76‑1 (MQ=255) ttttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTaa > 2:3540147/1‑76 (MQ=255) tttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAAt > 1:2893221/1‑76 (MQ=255) tttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAAt < 1:4168484/76‑1 (MQ=255) tttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAAt < 2:1844213/76‑1 (MQ=255) tttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAAt < 2:341737/76‑1 (MQ=255) tttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAAt < 2:2444799/76‑1 (MQ=255) tttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAAt > 2:3369981/1‑76 (MQ=255) tttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAAt < 1:4018324/76‑1 (MQ=255) ttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATc < 1:2306747/76‑1 (MQ=255) ttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATc > 2:25109/1‑76 (MQ=255) ttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATc < 1:4108464/76‑1 (MQ=255) ttGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATc > 1:3662421/1‑76 (MQ=255) tGAGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCg > 2:3354905/1‑76 (MQ=255) aGGGATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGcc > 2:2297064/1‑76 (MQ=255) | AAACTGTATTTTTCTTTCTGCGAGTTAACGCGTTGCCTTTCTGGGTAAATAACGCGCTTTTGGTTTTTTGAGGAATAGTAATGACGAAACATGCCAGGTTTTTCCTCCTGCCCTCCTTTATTCTGATCTCCGCGGCTTTAATCGCC > REL606/3341675‑3341820 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |