| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,513 | C→T | T553I (ACT→ATT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,513 | 0 | C | T | 99.4% | 682.3 / ‑4.7 | 169 | T553I (ACT→ATT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (92/76); total (93/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
Only 100 of 179 total aligned reads displayed.
CGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGT > REL606/4162440‑4162585 | cgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTc > 2:3296833/1‑76 (MQ=255)cgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTc < 2:2439057/76‑1 (MQ=255) gtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCa < 2:2625654/76‑1 (MQ=255) tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCAc > 1:3888197/1‑76 (MQ=255) aTCTCCTCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACt < 1:4969950/76‑1 (MQ=255) aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACCCCCGATTCACt > 1:173158/1‑76 (MQ=255) aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACt > 2:4885104/1‑76 (MQ=255) aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACt > 1:3509988/1‑76 (MQ=255) aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACt > 2:2323561/1‑76 (MQ=255) tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTa > 1:1507348/1‑76 (MQ=255) tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTa < 2:201570/76‑1 (MQ=255) tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTa < 1:3662987/76‑1 (MQ=255) tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTa > 2:3135477/1‑76 (MQ=255) ctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTAc < 2:2496935/76‑1 (MQ=255) tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACg > 2:4936255/1‑76 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACgg > 2:200691/1‑76 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACgg > 2:4360530/1‑76 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACgg < 1:4698947/76‑1 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTAAgg > 2:2908744/1‑76 (MQ=255) cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg < 1:397704/76‑1 (MQ=255) cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg < 1:4271596/76‑1 (MQ=255) cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg < 2:221096/76‑1 (MQ=255) cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg > 2:386823/1‑76 (MQ=255) cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg < 1:2079802/76‑1 (MQ=255) tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGt > 2:748842/1‑76 (MQ=255) ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt > 2:4106994/1‑76 (MQ=255) ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt > 2:2182735/1‑76 (MQ=255) ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt < 1:3913251/76‑1 (MQ=255) ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt > 2:4643680/1‑76 (MQ=255) ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt < 2:3943558/76‑1 (MQ=255) gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATg > 1:926431/1‑76 (MQ=255) gCCAAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTCCGAGACGTACACCCGATTCACTACGGTCGCGTATg < 1:293228/76‑1 (MQ=255) ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTc < 2:708800/76‑1 (MQ=255) ccAGGCGGTCTGACCCGCGAACGTGCAGGCTTCGGCGTTCGCGACGTACACCCGATTCACTACGCTCGCGAATGTc > 2:3524365/1‑76 (MQ=255) cAGGCTGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc < 2:4752380/76‑1 (MQ=255) cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc > 2:2939333/1‑76 (MQ=255) cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc < 2:1882296/76‑1 (MQ=255) cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc > 2:241282/1‑76 (MQ=255) cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc < 1:2110310/76‑1 (MQ=255) aGGCGGTCTGTCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCa < 1:3402581/76‑1 (MQ=255) aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCa < 2:3066665/76‑1 (MQ=255) gCGGTCTGCCCCGTGAACGTGCAGGCTTCGAGGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAAt < 1:4518246/76‑1 (MQ=255) gCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAAt < 1:2052046/76‑1 (MQ=255) cGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATc < 2:811130/76‑1 (MQ=255) cGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATc < 2:3570719/76‑1 (MQ=255) ggTCTGACCCGTGAGCGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCg > 1:4134038/1‑76 (MQ=255) ggTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCg > 2:3439359/1‑76 (MQ=255) gTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGa > 1:1254598/1‑76 (MQ=255) gTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGa > 2:4455706/1‑76 (MQ=255) cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGaaa > 2:3179141/1‑76 (MQ=255) cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACCACGGTGGCGTATGTCCGATGGaaa > 1:4412811/1‑76 (MQ=255) tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAc < 2:3836387/76‑1 (MQ=255) tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAc > 1:84490/1‑76 (MQ=255) gACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAcc > 1:1812615/1‑76 (MQ=255) gACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAcc > 2:4808882/1‑76 (MQ=255) gACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAcc > 1:1157054/1‑76 (MQ=255) ccGTGAACGTGCAGGCTTCGAAGTTTGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCt < 1:1272523/76‑1 (MQ=255) ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCt < 1:1827474/76‑1 (MQ=255) gTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa < 2:4200073/76‑1 (MQ=255) gTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa < 1:3315876/76‑1 (MQ=255) tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGac > 2:1251481/1‑75 (MQ=255) tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa < 2:4556910/76‑1 (MQ=255) tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa < 1:4582608/76‑1 (MQ=255) gAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg > 1:2731235/1‑76 (MQ=255) aaCGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAgg > 1:4186324/1‑76 (MQ=255) gTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc > 2:3633573/1‑76 (MQ=255) tGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg > 1:3247503/1‑76 (MQ=255) gCAGGTTTCGAAGTCCGAGCCGTCCACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa < 1:2759496/76‑1 (MQ=255) gCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa > 1:4632475/1‑76 (MQ=255) gCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa > 1:656226/1‑76 (MQ=255) cAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa < 1:3688427/76‑1 (MQ=255) cAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa > 2:4627804/1‑76 (MQ=255) cAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa > 1:286550/1‑76 (MQ=255) aGGCTTCGAAGTTCGAGACGTACGCCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 1:1035966/76‑1 (MQ=255) aGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 1:2401405/76‑1 (MQ=255) aGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 2:2539168/76‑1 (MQ=255) aGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 1:1185407/76‑1 (MQ=255) aGGCTTCAAAGTGCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 1:4798099/76‑1 (MQ=255) gCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAt > 2:2183924/1‑76 (MQ=255) cTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc > 1:2171685/1‑76 (MQ=255) aaGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt > 2:1481523/1‑76 (MQ=255) aaGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt < 1:106115/76‑1 (MQ=255) aaGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt > 2:2847811/1‑76 (MQ=255) aGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg < 2:4208988/76‑1 (MQ=255) aGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg > 2:1579340/1‑76 (MQ=255) aGTGCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg < 1:160297/76‑1 (MQ=255) gTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGa > 1:1951387/1‑76 (MQ=255) cGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa > 1:4839070/1‑76 (MQ=255) cGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 1:3244917/76‑1 (MQ=255) cGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 2:1861052/76‑1 (MQ=255) cGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 2:2748249/76‑1 (MQ=255) cGAGACGGACACCGGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 1:4092924/76‑1 (MQ=255) agaCGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAAc > 2:4316244/1‑76 (MQ=255) aCGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc > 2:2131754/1‑76 (MQ=255) aCGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc > 1:1981344/1‑76 (MQ=255) gTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc < 2:3740171/76‑1 (MQ=255) gTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc < 2:4930497/76‑1 (MQ=255) acacCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg > 1:3172219/1‑76 (MQ=255) cccGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc < 1:3933278/76‑1 (MQ=255) cccGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc > 2:3526111/1‑76 (MQ=255) | CGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGT > REL606/4162440‑4162585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |