| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 649,574 | T→C | K410E (AAA→GAA) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 649,574 | 0 | T | C | 99.2% | 490.0 / ‑4.2 | 118 | K410E (AAA→GAA) | mrdA | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (64/53); total (65/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
CATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTC > REL606/649501‑649646 | cATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGGAATGACCATAACCGAATTCAc > 1:4824211/1‑76 (MQ=255) aTTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCAcc < 2:217680/76‑1 (MQ=255) ttCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCAccc < 2:2699481/76‑1 (MQ=255) agggTAGGGTTTTTGCAGGAGCGGTCTTCCGCCAGGGCGATACCGGTGTAATGACCATACCCGAATTCACgcgtcc < 2:3250616/75‑1 (MQ=255) tAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCTATCCATTc > 2:4418596/1‑76 (MQ=255) tAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTc < 1:4242462/76‑1 (MQ=255) aGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCg < 2:4617214/76‑1 (MQ=255) aGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCg < 2:2064472/76‑1 (MQ=255) aGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCg > 2:3998150/1‑76 (MQ=255) aGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCg > 1:1923748/1‑76 (MQ=255) ggCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCgg < 1:2664685/76‑1 (MQ=255) gCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGga > 2:3141257/1‑76 (MQ=255) cATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGgag > 1:4044153/1‑76 (MQ=255) tGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGagag > 1:2364619/1‑76 (MQ=255) tGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGagag < 2:608846/76‑1 (MQ=255) tGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGagag > 2:1185842/1‑76 (MQ=255) ttGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGc > 1:5031825/1‑76 (MQ=255) ccGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGcgat > 1:5108168/1‑76 (MQ=255) ccGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGcgat > 2:4000550/1‑76 (MQ=255) ggAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGcgatcg > 2:4282594/1‑76 (MQ=255) gAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGcgatcga > 1:2720860/1‑76 (MQ=255) gAACGTTCTTCCGCCAGGTCGATACCAGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGcgatcga > 1:4486901/1‑76 (MQ=255) ttcttcCGCCAGGTGGATACCGGTGTAATGACCATAACGGAATTCACTCATCCATTCGGAGAGGCGATGGATtccc > 2:2083794/1‑76 (MQ=255) ttcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATctcc > 2:4737280/1‑76 (MQ=255) ttcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATcccc > 2:3347077/1‑76 (MQ=255) ttcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATcccc < 2:958435/76‑1 (MQ=255) tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCa > 1:3481713/1‑76 (MQ=255) tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCa > 1:1716111/1‑76 (MQ=255) tcttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCa > 1:1889727/1‑76 (MQ=255) cttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCat < 2:2918684/76‑1 (MQ=255) cttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCat > 1:2117385/1‑76 (MQ=255) ttcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCata < 2:4846873/76‑1 (MQ=255) tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCatat > 1:313002/1‑76 (MQ=255) tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCatat > 1:3675091/1‑76 (MQ=255) tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCatat < 1:3814497/76‑1 (MQ=255) tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCatat < 2:3174524/76‑1 (MQ=255) ccGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATc < 2:4295540/76‑1 (MQ=255) ccGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATc > 1:4668282/1‑76 (MQ=255) ccGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATc < 1:4031398/76‑1 (MQ=255) ccGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATc > 2:4950770/1‑76 (MQ=255) cGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCg < 1:3629196/76‑1 (MQ=255) cAGGTCGATACCGGTGTAATGACCATAACCGAATTCCCCCATCCAATCGGAGAGGCGATCGATCCCCATATCGTAg < 2:3764204/76‑1 (MQ=255) cAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAg > 2:1546598/1‑76 (MQ=255) cAGGTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAg > 1:2064204/1‑76 (MQ=255) aGGTCGATACCGGTGTAATGACCATAACCGAATTCCCCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAgg < 1:1033223/76‑1 (MQ=255) gTCGATACCGGTGTAATGCCCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGcc < 1:603555/76‑1 (MQ=255) gTCGATACCGGTGTAATGACCATAACCGAATTCGCCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGcc < 1:5104492/76‑1 (MQ=255) gTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGcc < 1:2022046/76‑1 (MQ=255) gTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGcc > 1:3748833/1‑76 (MQ=255) gTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGcc > 1:3746530/1‑76 (MQ=255) gTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGcc < 1:3426532/76‑1 (MQ=255) gTCGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGcc > 2:1135395/1‑76 (MQ=255) cGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc > 1:2750043/1‑76 (MQ=255) cGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc > 2:4196422/1‑76 (MQ=255) cGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc > 1:3812896/1‑76 (MQ=255) cGATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAc > 1:1603518/1‑76 (MQ=255) gATACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCAcc > 1:2120258/1‑76 (MQ=255) tACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTg < 2:4421504/76‑1 (MQ=255) tACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTg < 1:340793/76‑1 (MQ=255) tACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTg < 1:3930135/76‑1 (MQ=255) tACCGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTg < 1:4460197/76‑1 (MQ=255) cGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATa > 1:474794/1‑76 (MQ=255) cGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATa < 2:4925391/76‑1 (MQ=255) cGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATa < 1:1871376/76‑1 (MQ=255) cGGTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATa > 2:988881/1‑76 (MQ=255) ggTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag < 1:3170555/76‑1 (MQ=255) ggTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag < 2:3365507/76‑1 (MQ=255) ggTGTAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATag < 1:1233233/76‑1 (MQ=255) gtgtAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATaga < 2:792516/76‑1 (MQ=255) tgtAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATagaa > 2:3871185/1‑76 (MQ=255) gtAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATagaag > 1:4463618/1‑76 (MQ=255) gtAATGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATagaag < 1:1546598/76‑1 (MQ=255) aaTGACCATAACCGATTTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCTCCTGATAgaagaa < 2:2999250/76‑1 (MQ=255) aaTGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAgaagaa < 1:5133385/76‑1 (MQ=255) aaTGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAgaagaa < 1:137718/76‑1 (MQ=255) aaTGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAgaagaa < 2:1761439/76‑1 (MQ=255) aaTGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAgaagaa > 2:1634980/1‑76 (MQ=255) aaTGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAgaagaa > 2:1434715/1‑76 (MQ=255) aTGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGaagaag > 2:2353873/1‑76 (MQ=255) aTGACAATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGaagaag > 2:962772/1‑76 (MQ=255) tGACCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAgg < 2:3865420/76‑1 (MQ=255) aCCATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTa > 1:1591439/1‑76 (MQ=255) ccATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTAt > 1:1048627/1‑76 (MQ=255) ccATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTAt < 1:2802706/76‑1 (MQ=255) ccATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTAt > 1:353433/1‑76 (MQ=255) cATAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATc > 1:1711497/1‑76 (MQ=255) aTAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcc > 2:212302/1‑76 (MQ=255) aTAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATcc > 1:1526416/1‑76 (MQ=255) tAACCGAATTCCCCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCg < 2:1896160/76‑1 (MQ=255) tAACCGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCg > 1:436896/1‑76 (MQ=255) ccGAATTCCCCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAg < 2:4424918/76‑1 (MQ=255) ccGAATTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAg > 2:2898904/1‑76 (MQ=255) gAATTCCCCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAt < 2:3748833/76‑1 (MQ=255) gAATTCACCCATCCATGGGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAt < 2:2187493/76‑1 (MQ=255) aaTTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt > 2:4830629/1‑76 (MQ=255) aaTTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt > 1:1969153/1‑76 (MQ=255) aaTTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAtt < 2:5166638/76‑1 (MQ=255) aTTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttc < 1:922023/76‑1 (MQ=255) aTTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttc < 1:2403258/76‑1 (MQ=255) aTTCACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttc > 2:2334579/1‑76 (MQ=255) | CATTCGCGGGTAGGCATGTTGCCGGAACGTTCTTCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTC > REL606/649501‑649646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |