BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,290,813	C→T	Q260* (CAG→TAG)	hnr →	response regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,290,813	0	C	T	100.0%	525.5 / NA	137	Q260* (CAG→TAG)	hnr	response regulator of RpoS
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (76/61); total (76/61)

Only 100 of 140 total aligned reads displayed.

TCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATG  >  REL606/1290740‑1290885

tCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAg                                                                        <  2:3028491/76‑1 (MQ=255)

 catcGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGGCAGGCCAATCTGCCGGGGTAGt                                                                       <  2:877984/73‑1 (MQ=255)

 cAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGt                                                                       <  2:852750/76‑1 (MQ=255)

  aaCGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGtt                                                                      >  1:5082984/1‑76 (MQ=255)

   aCGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGttt                                                                     <  2:2744430/76‑1 (MQ=255)

   aCGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGttt                                                                     <  1:2458663/76‑1 (MQ=255)

      gTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCg                                                                  >  2:3511430/1‑76 (MQ=255)

      gTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCg                                                                  <  2:2214967/76‑1 (MQ=255)

        tGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCt                                                                >  2:3687046/1‑76 (MQ=255)

        tGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCt                                                                <  2:54515/76‑1 (MQ=255)

        tGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCt                                                                >  1:1410218/1‑76 (MQ=255)

         gCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTa                                                               <  2:3515561/76‑1 (MQ=255)

          ccAGAGTTGGCCGCGTTATTGAAGCAGGTAAACCATTTACCCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTAt                                                              <  2:2101513/76‑1 (MQ=255)

            agagTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTa                                                            <  1:3203983/76‑1 (MQ=255)

            agagTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTa                                                            >  2:2868466/1‑76 (MQ=255)

             gagTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAg                                                           <  1:4214276/76‑1 (MQ=255)

             gagTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAg                                                           <  1:1681403/76‑1 (MQ=255)

              agTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGt                                                          <  1:2044720/76‑1 (MQ=255)

              agTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGt                                                          >  2:1153800/1‑76 (MQ=255)

                ttGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTg                                                        <  1:3761685/76‑1 (MQ=255)

                 tGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTgg                                                       >  2:1377179/1‑76 (MQ=255)

                 tGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTgg                                                       <  2:1872335/76‑1 (MQ=255)

                 tGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTgg                                                       <  1:457574/76‑1 (MQ=255)

                  gggCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGc                                                      >  1:3644457/1‑76 (MQ=255)

                   ggCGCGTTTTTGAAGCAGGGAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCt                                                     <  1:4285928/76‑1 (MQ=255)

                   ggCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCt                                                     <  2:1486793/76‑1 (MQ=255)

                   ggCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCt                                                     <  1:3948519/76‑1 (MQ=255)

                    gcgcGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCta                                                    >  1:274554/1‑76 (MQ=255)

                     cgcgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtat                                                   >  2:1841242/1‑76 (MQ=255)

                     cgcgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtat                                                   <  1:2780530/76‑1 (MQ=255)

                      gcgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtatt                                                  <  1:3795616/76‑1 (MQ=255)

                      gcgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtatt                                                  <  2:2012647/76‑1 (MQ=255)

                      gcgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtatt                                                  >  2:2771782/1‑76 (MQ=255)

                      gcgTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtatt                                                  <  1:4467647/76‑1 (MQ=255)

                        gTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtattat                                                <  1:3807311/76‑1 (MQ=255)

                        gTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtattat                                                <  2:1694507/76‑1 (MQ=255)

                        gTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtattat                                                >  2:2520710/1‑76 (MQ=255)

                        gTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCtattat                                                <  1:740016/76‑1 (MQ=255)

                         ttATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatc                                               <  1:4336131/76‑1 (MQ=255)

                         ttATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatc                                               <  1:4648237/76‑1 (MQ=255)

                          tATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatca                                              >  2:2336705/1‑76 (MQ=255)

                          tATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatca                                              >  1:4588524/1‑76 (MQ=255)

                          tATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatca                                              >  2:4799336/1‑76 (MQ=255)

                           aTTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatcat                                             >  1:1987204/1‑76 (MQ=255)

                           aTTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatcat                                             <  1:1671564/76‑1 (MQ=255)

                           aTTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatcat                                             >  1:2114229/1‑76 (MQ=255)

                           aTTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatcat                                             >  1:3574233/1‑76 (MQ=255)

                           aTTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatcat                                             >  1:2271265/1‑76 (MQ=255)

                           aTTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatcat                                             >  2:5038260/1‑76 (MQ=255)

                             tGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATcg                                           <  1:1633198/76‑1 (MQ=255)

                               aaGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATcgcg                                         <  1:1719411/76‑1 (MQ=255)

                                aGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGa                                        <  2:3964157/76‑1 (MQ=255)

                                  cAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAAc                                      >  2:3600792/1‑76 (MQ=255)

                                    ggTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTg                                    >  1:2671795/1‑76 (MQ=255)

                                    ggTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTg                                    >  1:3792495/1‑76 (MQ=255)

                                      tAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaa                                  >  2:2204455/1‑76 (MQ=255)

                                       aaaCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGcaa                                 >  2:4142422/1‑76 (MQ=255)

                                       aaaCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaaa                                 >  1:4563205/1‑76 (MQ=255)

                                        aaCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaaaa                                >  1:4886923/1‑76 (MQ=255)

                                             tttACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAAtctc                           >  2:804625/1‑76 (MQ=255)

                                              ttACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCa                          <  2:4289776/76‑1 (MQ=255)

                                              ttACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCa                          <  1:1845390/76‑1 (MQ=255)

                                               tACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAt                         >  2:1216192/1‑76 (MQ=255)

                                               tACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAt                         >  1:860335/1‑76 (MQ=255)

                                               tACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAt                         <  2:574142/76‑1 (MQ=255)

                                                  tCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt                      >  1:5008443/1‑76 (MQ=255)

                                                  tCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt                      >  2:4932775/1‑76 (MQ=255)

                                                  tCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt                      >  1:2919556/1‑76 (MQ=255)

                                                   ccGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTg                     <  1:2759233/76‑1 (MQ=255)

                                                   ccGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTg                     >  2:3776140/1‑76 (MQ=255)

                                                   ccGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTg                     >  2:2956308/1‑76 (MQ=255)

                                                   ccGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTg                     >  2:1459938/1‑76 (MQ=255)

                                                   ccGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTg                     >  2:2327545/1‑76 (MQ=255)

                                                   ccGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTg                     >  2:2056027/1‑76 (MQ=255)

                                                    cGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTgg                    <  2:4294876/76‑1 (MQ=255)

                                                     gTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGt                   >  1:3767913/1‑76 (MQ=255)

                                                     gTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGt                   <  2:3574233/76‑1 (MQ=255)

                                                      tCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGtt                  <  1:138844/76‑1 (MQ=255)

                                                      tCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGtt                  >  2:842041/1‑76 (MQ=255)

                                                       cAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGttt                 >  2:3815351/1‑76 (MQ=255)

                                                       cAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGttt                 >  1:938861/1‑76 (MQ=255)

                                                       cAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGttt                 >  1:2196921/1‑76 (MQ=255)

                                                       cAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGttt                 >  1:1437410/1‑76 (MQ=255)

                                                        aGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTc                >  2:1395748/1‑76 (MQ=255)

                                                            cAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCg            >  2:3114397/1‑76 (MQ=255)

                                                            cAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCg            >  2:310446/1‑76 (MQ=255)

                                                                 tGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCt       >  2:4489630/1‑76 (MQ=255)

                                                                 tGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCt       >  1:4472019/1‑76 (MQ=255)

                                                                 tGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCt       <  2:4817446/76‑1 (MQ=255)

                                                                  gCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTg      >  2:4980631/1‑76 (MQ=255)

                                                                  gCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTg      >  1:2600126/1‑76 (MQ=255)

                                                                  gCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTg      >  1:2403894/1‑76 (MQ=255)

                                                                  gCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTg      >  2:832080/1‑76 (MQ=255)

                                                                     ggggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAAt   <  1:1013471/76‑1 (MQ=255)

                                                                     ggggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAAt   <  1:4697608/76‑1 (MQ=255)

                                                                     ggggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAAt   <  1:4053796/76‑1 (MQ=255)

                                                                      gggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg  <  2:3497456/76‑1 (MQ=255)

                                                                      gggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg  >  1:129439/1‑76 (MQ=255)

                                                                      gggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg  <  1:2224874/76‑1 (MQ=255)

                                                                      gggTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATg  >  1:414096/1‑76 (MQ=255)

TCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATG  >  REL606/1290740‑1290885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`