| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,060,017 | C→T | G127S (GGC→AGC) | wcaG ← | bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,060,017 | 0 | C | T | 98.0% | 370.2 / ‑4.1 | 101 | G127S (GGC→AGC) | wcaG | bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (34/65); total (36/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
TTGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAACA > REL606/2059944‑2060088 | ttGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGctct < 1:5109703/76‑1 (MQ=255) tGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTg < 2:1443821/76‑1 (MQ=255) tGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTg < 2:193118/76‑1 (MQ=255) tGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTg < 2:3715218/76‑1 (MQ=255) gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGctcttc > 1:4821441/1‑74 (MQ=255) gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc > 1:2959896/1‑76 (MQ=255) gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc < 2:1056456/76‑1 (MQ=255) gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc < 2:1129873/76‑1 (MQ=255) gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc > 1:3514407/1‑76 (MQ=255) gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc > 2:4440087/1‑76 (MQ=255) tAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGca < 1:1565950/76‑1 (MQ=255) aaGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaa < 1:2002541/76‑1 (MQ=255) aaGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaa > 2:2277019/1‑76 (MQ=255) aGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaac > 2:4036504/1‑76 (MQ=255) aGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaac < 1:2428682/76‑1 (MQ=255) gATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaaca < 1:3068364/76‑1 (MQ=255) aTTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaacaa < 2:4431252/76‑1 (MQ=255) aTTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaacaa > 2:3540231/1‑76 (MQ=255) ttCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCaacaac > 2:1754214/1‑76 (MQ=255) tCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACt > 1:1033500/1‑76 (MQ=255) tCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACt > 2:1620130/1‑76 (MQ=255) caGTTTGATCCTGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGGGCTCTGCAACAACTCGCtt > 1:135987/1‑76 (MQ=255) caGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCtt < 2:3708006/76‑1 (MQ=255) caGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCtt > 1:4117467/1‑76 (MQ=255) aGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCttt > 2:2062999/1‑76 (MQ=255) aGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCttt < 2:4669734/76‑1 (MQ=255) gTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTc < 1:3124382/76‑1 (MQ=255) tttGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCt > 2:1161162/1‑76 (MQ=255) ttGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTg > 1:1040552/1‑76 (MQ=255) tGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCCGCGTGCTCCGCAACAACTCGCTTTCCGc > 1:4631677/1‑76 (MQ=255) tGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGc > 1:357344/1‑76 (MQ=255) tGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGc > 1:4363966/1‑76 (MQ=255) gATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGcc > 1:3436295/1‑76 (MQ=255) gATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGcc < 2:2560340/76‑1 (MQ=255) cccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATc > 2:3779974/1‑76 (MQ=255) cccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATc < 1:1583036/76‑1 (MQ=255) cccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATc < 1:4379992/76‑1 (MQ=255) cccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATc > 2:3636516/1‑76 (MQ=255) ccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCg > 2:2762652/1‑76 (MQ=255) cagCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCgg > 1:42070/3‑76 (MQ=255) ggCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGc < 2:4987454/76‑1 (MQ=255) gCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCt > 1:976717/1‑76 (MQ=255) gATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gt < 1:3911836/76‑1 (MQ=255) gATTTTGGCAATAGCATAAGGCTCGTTAGTCAGCTCCAGCGAGCTCTGAAACAACTCGCTTTCTGGCATCGGCT‑gg > 1:405244/1‑75 (MQ=255) ttttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCCTCGGCT‑gggt > 1:1809986/1‑73 (MQ=255) ttttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑gggt > 2:653752/1‑73 (MQ=255) ttttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gttt < 1:5029003/76‑1 (MQ=255) ttttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gttt < 1:2277019/76‑1 (MQ=255) tttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gtttt < 2:574604/76‑1 (MQ=255) tttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gtttt < 2:2654707/76‑1 (MQ=255) ttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTg < 1:1345452/76‑1 (MQ=255) ttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTg < 2:1406729/76‑1 (MQ=255) ggCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGcc < 2:4177867/76‑1 (MQ=255) cAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTGcctt > 1:5048041/1‑74 (MQ=255) cAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCCGCT‑GGTTTGCCAg > 1:4578486/1‑76 (MQ=255) tAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCTGGTTTTGCCGGtt > 1:1513910/1‑76 (MQ=255) tAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGttt < 2:2492772/76‑1 (MQ=255) aGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGTT‑GTTTTGCCAGTTTc < 2:4332264/76‑1 (MQ=255) aGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCTGGTTTTTCCGGttt > 2:2135125/1‑76 (MQ=255) aGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTc < 1:1139947/76‑1 (MQ=255) aGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTc < 2:4621668/76‑1 (MQ=255) gCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCg < 1:121233/76‑1 (MQ=255) tAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGt < 1:1437211/76‑1 (MQ=255) tAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGt < 1:547606/76‑1 (MQ=255) tAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGt < 2:4036628/76‑1 (MQ=255) tAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGt < 1:4876345/76‑1 (MQ=255) aaGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTa < 1:2907583/76‑1 (MQ=255) aGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAg < 2:2977718/76‑1 (MQ=255) aGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAg < 1:656359/76‑1 (MQ=255) gCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGAt < 1:2924108/76‑1 (MQ=255) gCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTGTCCGTTTCGGGgagaa > 1:4562173/1‑75 (MQ=255) cTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATa < 2:199555/76‑1 (MQ=255) cGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTGCCAGTTTTTGGGAGAGACa > 2:4886136/1‑76 (MQ=255) gTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAg < 1:653752/76‑1 (MQ=255) gTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAg < 1:3238553/76‑1 (MQ=255) gTTAGTCGGCTCCAGCGTGCTCGGCAACACCTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAg < 1:47151/76‑1 (MQ=255) gTTAGGCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAg < 2:2992344/76‑1 (MQ=255) ttAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAgg < 1:2931248/76‑1 (MQ=255) ttAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAgg < 1:562975/76‑1 (MQ=255) tAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGa < 2:1754916/76‑1 (MQ=255) aGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTTTCAGTTTTGGGGGGATAAAGGAc > 1:3342551/1‑76 (MQ=255) aGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTTGCAGCTTTGGGGAAATACAGGAc > 1:3409645/1‑76 (MQ=255) aGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGACATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGAc < 1:2657559/76‑1 (MQ=255) ggcGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACg < 2:2931571/74‑1 (MQ=255) gTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACg < 1:887709/76‑1 (MQ=255) gTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACg < 1:1620279/76‑1 (MQ=255) cGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGAt < 2:119846/76‑1 (MQ=255) ggCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATc < 1:2209350/76‑1 (MQ=255) cTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCg < 2:152102/76‑1 (MQ=255) cTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCg < 1:1638064/76‑1 (MQ=255) ccAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCgag < 2:39239/76‑1 (MQ=255) ccAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTGTCCGTTTCCGGTGGGAACAGGGCGGTCCcag > 2:1842624/1‑76 (MQ=255) cAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCgaga < 1:3697463/76‑1 (MQ=255) aGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGaa < 1:3111110/76‑1 (MQ=255) gCGTGCTCTGCAACACCTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGaaa < 1:1629757/76‑1 (MQ=255) gCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGaaa < 1:4650494/76‑1 (MQ=255) ggTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAAc < 2:3385967/75‑1 (MQ=255) cGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAAc < 2:3363830/76‑1 (MQ=255) cGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAAc < 2:2869548/76‑1 (MQ=255) gTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAAca < 1:1696448/76‑1 (MQ=255) | TTGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAACA > REL606/2059944‑2060088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |