BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	418.8 / NA	115	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (39/76); total (39/76)

Only 100 of 131 total aligned reads displayed.

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATcc                                                                        <  1:3101224/76‑1 (MQ=255)

 ccGATGCATCCGGCGTCCAGGCGCGGCTGGGCTTGCTG‑TT‑GGGAATCAGCCCCCATAACACGAAAACCCCAAAccc                                                                       >  1:3186699/1‑76 (MQ=255)

   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     <  1:575773/76‑1 (MQ=255)

   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     <  1:1224279/76‑1 (MQ=255)

   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     <  1:2957677/76‑1 (MQ=255)

   gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGGTG‑TC‑GGAAAACAGCCCCAAAAAAAACAAAAAGCCAATCCcca                                                                     >  1:4326131/1‑76 (MQ=255)

   gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTTC‑GGAAA‑CAACCCCCAAAACAACAAAAACCCAAAccccc                                                                     >  1:2420671/1‑75 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTG‑TT‑GGGAAACAGGCCCCATAAACACAAAAACCCAAACCcccccaa                                                                 >  2:4565089/1‑75 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTG‑TCGGGAAAAAACCCCCAAAAAAAGCAAAACCCCAACCCccccca                                                                  >  2:309849/1‑76 (MQ=255)

       cATCCGGCGTCCAGGCGCGGCTGGGCTGGCTG‑TC‑GGGAATAAGCCCCCATAAAAGCAAAAAGCCAAACccccccca                                                                 >  2:719296/1‑74 (MQ=255)

        acccGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                                <  1:832435/74‑1 (MQ=255)

        aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                                <  2:5076022/76‑1 (MQ=255)

        aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTG‑TC‑GGGAAACAGCCCCCAAAACAGCCAAAAACCCATCCcccccaca                                                                >  2:3164920/1‑74 (MQ=255)

         tCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               <  1:186422/76‑1 (MQ=255)

         tCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               <  2:394046/76‑1 (MQ=255)

          ccGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                              <  2:1952379/76‑1 (MQ=255)

          ccGGCGTCCAGGCGCGGCTGGGCTGGGTG‑GC‑GGGAAACAGGCCCCATAACAGCAAAACGCCCATGCCCCCCACATa                                                              >  2:2736386/1‑76 (MQ=255)

          ccGGCGTCCAGGCGCGGCTGGGCTGGCTG‑TC‑GGGAAACAGCCCCAATAAAAGCAAAAAGCCAATCCCCACCCGATa                                                              >  1:2716943/1‑76 (MQ=255)

            ggCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                            <  2:84772/76‑1 (MQ=255)

             gCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                           <  2:2606783/76‑1 (MQ=255)

              cGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:3413945/76‑1 (MQ=255)

              cGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                          <  2:270984/76‑1 (MQ=255)

                   aGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     <  1:3821619/76‑1 (MQ=255)

                     gcgcGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGc                                                   <  1:3290230/76‑1 (MQ=255)

                      cgcgGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGcc                                                  <  1:460392/76‑1 (MQ=255)

                       gcgGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                 <  1:3823568/76‑1 (MQ=255)

                        cgGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:5175773/76‑1 (MQ=255)

                        cgGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  2:3058234/76‑1 (MQ=255)

                         ggCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  1:4080909/76‑1 (MQ=255)

                         ggCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACCCCCGt                                               >  2:1070973/1‑76 (MQ=255)

                           cTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  1:4517678/1‑76 (MQ=255)

                            tGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:2798711/76‑1 (MQ=255)

                            tGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:3699607/1‑76 (MQ=255)

                            tGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:3616594/1‑76 (MQ=255)

                            tGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:3258121/76‑1 (MQ=255)

                            tGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:3526605/76‑1 (MQ=255)

                             ggTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  1:504619/1‑76 (MQ=255)

                              gTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          >  2:258286/1‑76 (MQ=255)

                              gTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  2:4229946/76‑1 (MQ=255)

                               tCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  2:203770/1‑76 (MQ=255)

                               tCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  1:5089364/76‑1 (MQ=255)

                               tCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  1:4688350/76‑1 (MQ=255)

                                cTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  1:2375680/76‑1 (MQ=255)

                                cTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:2377631/1‑76 (MQ=255)

                                cTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:4741788/1‑76 (MQ=255)

                                cTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  1:4710787/76‑1 (MQ=255)

                                cTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:337780/76‑1 (MQ=255)

                                cTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:4224503/1‑76 (MQ=255)

                                  ggCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  2:4023239/76‑1 (MQ=255)

                                  ggCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      >  2:3267853/1‑76 (MQ=255)

                                  ggCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      >  1:1906516/1‑76 (MQ=255)

                                  ggCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  2:1563382/76‑1 (MQ=255)

                                     tG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   <  2:2542809/76‑1 (MQ=255)

                                      g‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  1:1232718/76‑1 (MQ=255)

                                      g‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:2307281/76‑1 (MQ=255)

                                      g‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:1826924/76‑1 (MQ=255)

                                      g‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  1:1138106/1‑76 (MQ=255)

                                      g‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:4430911/76‑1 (MQ=255)

                                        tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAACCAGGCttt                                 <  2:471224/76‑1 (MQ=255)

                                        tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:4989472/76‑1 (MQ=255)

                                        tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:4594553/76‑1 (MQ=255)

                                        tC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  1:3706073/76‑1 (MQ=255)

                                        gc‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:4327861/75‑1 (MQ=255)

                                         c‑GGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  1:749664/76‑1 (MQ=255)

                                            gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:1157110/76‑1 (MQ=255)

                                            gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:2387233/76‑1 (MQ=255)

                                             aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:4114200/1‑76 (MQ=255)

                                             aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:3360859/1‑76 (MQ=255)

                                             aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             <  2:4844493/76‑1 (MQ=255)

                                             aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:4434020/1‑76 (MQ=255)

                                               aTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           <  2:5133043/76‑1 (MQ=255)

                                                tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  2:4822295/70‑1 (MQ=255)

                                                tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  1:4822295/1‑70 (MQ=255)

                                                tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          <  1:1140907/76‑1 (MQ=255)

                                                    ccccAATAACAGCAAAACGCCAATCCTCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  1:504458/1‑76 (MQ=255)

                                                    ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                      >  2:4853112/1‑76 (MQ=255)

                                                      ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                    >  2:2245805/1‑76 (MQ=255)

                                                         aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        >  1:3349435/1‑69 (MQ=255)

                                                         aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  1:3083639/1‑76 (MQ=255)

                                                         aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 <  2:2655746/76‑1 (MQ=255)

                                                          tAACAGCAAAACGGCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  1:3858140/76‑1 (MQ=255)

                                                             cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:857838/76‑1 (MQ=255)

                                                             cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:3686892/76‑1 (MQ=255)

                                                             cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:3104458/76‑1 (MQ=255)

                                                             cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:2240612/76‑1 (MQ=255)

                                                             cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:1225116/76‑1 (MQ=255)

                                                               gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  2:5106598/76‑1 (MQ=255)

                                                                 aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGGGCACCa         >  1:656449/1‑76 (MQ=255)

                                                                 aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:3382310/76‑1 (MQ=255)

                                                                  aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:3343270/76‑1 (MQ=255)

                                                                  aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:1569590/76‑1 (MQ=255)

                                                                   aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  2:2304418/1‑76 (MQ=255)

                                                                     cGCAAACCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGTGTTTTTACCGATGGCGCATCGCGCCCCAgccg     <  2:504619/76‑1 (MQ=255)

                                                                       ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  2:2364/1‑76 (MQ=255)

                                                                       ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   <  1:4855630/76‑1 (MQ=255)

                                                                        cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:4331871/1‑76 (MQ=255)

                                                                        cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:1856189/1‑76 (MQ=255)

                                                                        cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  1:4855167/1‑76 (MQ=255)

                                                                        cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:2941150/1‑76 (MQ=255)

                                                                        cAACCCCCACCAAATAAAGCATGACGCCTGTATTAAACAGGCTTTTTCCCGATGGCCCACCGCGCCCCAGCCGCca  <  2:1941405/76‑1 (MQ=255)

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTG‑TC‑GGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`