Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,195,257 (C)9→8 coding (110/837 nt) yeiG → predicted esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,195,2490C.86.4% 574.9 / 62.0 147coding (102/837 nt)yeiGpredicted esterase
Reads supporting (aligned to +/- strand):  ref base C (1/19);  new base . (68/59);  total (69/78)
Fisher's exact test for biased strand distribution p-value = 2.21e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

TTTTGAAGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTC  >  REL606/2195174‑2195324
                                                                           |                                                                           
ttttGAAGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTtctc                                                                             <  2:5681235/76‑1 (MQ=255)
  ttGAAGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTccc                                                                           >  1:8773678/1‑76 (MQ=255)
  ttGAAGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTccc                                                                           <  1:1747874/76‑1 (MQ=255)
   tGAAGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTcccc                                                                          <  2:1603799/76‑1 (MQ=255)
    gAAGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTccccc                                                                         <  2:8657308/76‑1 (MQ=255)
    gAAGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTccccc                                                                         >  1:3573148/1‑76 (MQ=255)
     aaGGCTGGCAGCAACGCTGGCTACACGACTCCAGCACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTaccccc                                                                        >  2:2026077/1‑76 (MQ=255)
     aaGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTcccccc                                                                        >  2:7353497/1‑76 (MQ=255)
     aaGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTcccccc                                                                        <  1:5908786/76‑1 (MQ=255)
      aGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTccccccc                                                                       <  2:6841436/76‑1 (MQ=255)
      aGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTccccccc                                                                       <  1:508675/76‑1 (MQ=255)
         cTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTcccccccctc                                                                    <  2:4753142/76‑3 (MQ=255)
         cTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTcccccccctc                                                                    <  1:2960922/76‑3 (MQ=255)
          tGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTcccccccctcg                                                                   >  2:4639445/1‑73 (MQ=255)
          tGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTcccccccctcg                                                                   >  1:8130744/1‑73 (MQ=255)
          tGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTcccccccctcg                                                                   >  2:7772941/1‑73 (MQ=255)
            gcagcaACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTg                                                                >  2:1804230/1‑76 (MQ=255)
            gcagcaACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTg                                                                <  2:6110841/76‑1 (MQ=255)
            gcagaaACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTg                                                                <  1:5940200/76‑1 (MQ=255)
             cagcaACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCCCCCCCTCGTg                                                                <  2:3228652/76‑1 (MQ=255)
             cagcaACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGa                                                               >  1:4851237/1‑76 (MQ=255)
               gcaACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATc                                                             <  1:1197259/76‑1 (MQ=255)
               gcaACGCTGGCGACACGACTCCAGTACCTTAAACTACCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATc                                                             >  2:2170492/1‑76 (MQ=255)
                caACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATca                                                            <  2:7974208/76‑1 (MQ=255)
                 aaCGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATcac                                                           <  1:911856/76‑1 (MQ=255)
                  aCGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATcaca                                                          <  2:4417722/76‑1 (MQ=255)
                  aCGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATcaca                                                          <  2:5831667/76‑1 (MQ=255)
                  aCGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATcaca                                                          <  2:5520768/76‑1 (MQ=255)
                    gCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACAcc                                                        >  1:7243239/1‑76 (MQ=255)
                     cTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACAccc                                                       >  2:7159753/1‑76 (MQ=255)
                     cTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACAccc                                                       <  1:7542633/76‑1 (MQ=255)
                      tGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACAcccc                                                      <  1:1228400/76‑1 (MQ=255)
                      tGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACAcccc                                                      <  2:2775517/76‑1 (MQ=255)
                      tGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACAcccc                                                      <  1:28671/76‑1 (MQ=255)
                         cGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGcc                                                   >  2:2933388/1‑76 (MQ=255)
                          gACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGcca                                                  <  1:7457298/76‑1 (MQ=255)
                          gACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGcca                                                  <  1:3442834/76‑1 (MQ=255)
                           acacGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGccac                                                 >  2:1633805/1‑76 (MQ=255)
                            cacGCCCCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGccacc                                                <  2:3579342/76‑1 (MQ=255)
                            cacGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGTTCACCCCCCGccacc                                                <  2:5651272/76‑1 (MQ=255)
                             ccGACTCCAGAACCTGAAACTCCCCGAGGACGTGCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGccacca                                               <  2:9001183/75‑1 (MQ=255)
                             acGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCCccacca                                               >  1:4026246/1‑76 (MQ=255)
                              cGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAg                                              <  1:1462063/76‑1 (MQ=255)
                              cGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAg                                              >  2:756399/1‑76 (MQ=255)
                              cGACGCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAg                                              <  1:6594495/76‑1 (MQ=255)
                                ccTCCCGTACCTTAACCTGCCCGATGCCGTTCAGTATCTTTCT‑CCCCCCCCTCGGGATCACACCCCGCCACCAGTg                                            <  2:6545510/75‑1 (MQ=255)
                                aCTCCAGTCCCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTg                                            <  1:4662924/76‑1 (MQ=255)
                                 cTCCATAACCTTAAACTGCCCGATGACGTTCAGTATCTTCCT‑CCCCCCCCTCGTGATCACACCCCGCCACCACTGc                                           >  2:2228982/1‑76 (MQ=255)
                                  tCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCCCCCCC‑CCTGATCACACCCCGCCACCCGTGCt                                          >  2:4101122/1‑76 (MQ=255)
                                  cccAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCt                                          <  2:8338259/75‑1 (MQ=255)
                                  cccAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCt                                          <  2:5433707/75‑1 (MQ=255)
                                     aGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTa                                       >  2:5295830/1‑76 (MQ=255)
                                       tcccTGAAACTGCCCAATGGCGTGCATTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACt                                     <  2:1444274/74‑1 (MQ=255)
                                       tACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACt                                     <  2:1838756/76‑1 (MQ=255)
                                       tACCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACt                                     >  1:8987951/1‑76 (MQ=255)
                                       tACCTTAAACTGCCCGATGACGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACCCCCCGCCACCAGTGCTGTACt                                     <  1:5171140/76‑1 (MQ=255)
                                       tACCTTAAACTGCCCGATGACGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACt                                     <  1:2803365/76‑1 (MQ=255)
                                       tACCTTAAACTGCCCGATGACGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACt                                     <  2:2390975/76‑1 (MQ=255)
                                        aCCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTg                                    <  1:8744770/76‑1 (MQ=255)
                                        aCCTTAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTg                                    >  1:1658240/1‑76 (MQ=255)
                                          cTTAACCTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGc                                  <  2:8781293/76‑1 (MQ=255)
                                           ttAACCTGCCCGATGACGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACCCCCCGCCACCAGTGCTGTACTGGCt                                 <  1:5235032/76‑1 (MQ=255)
                                           ttAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCt                                 >  2:3847058/1‑76 (MQ=255)
                                            tAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCtt                                >  1:7600958/1‑76 (MQ=255)
                                            tAAACTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCtt                                >  1:6761264/1‑76 (MQ=255)
                                             aaaCTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCttt                               >  2:7169320/1‑76 (MQ=255)
                                             aaaCTCCCCGATGACGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCttt                               <  1:6946811/76‑1 (MQ=255)
                                              aaCTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTc                              >  1:1741238/1‑76 (MQ=255)
                                               aCTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTcc                             >  1:4389249/1‑76 (MQ=255)
                                                cTGCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACCCCCCGCCACCAGTGCTGTACTGGCTTTCCg                            <  2:870552/76‑1 (MQ=255)
                                                 tGCCCGAGGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACCCCCCGCCACCAGTGCTGTACTGGCTTTCCgg                           <  2:4825616/76‑1 (MQ=255)
                                                  gCCCGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGa                          >  2:6879883/1‑76 (MQ=255)
                                                    cggATGCCGTTCAGTTTCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGAtt                        <  2:3264215/74‑1 (MQ=255)
                                                    ccGATGACGTTCAGTATCTTTCTCCCCCCCCCTCGTGATCACCCCCCGCCCCCAGTGCTGTACTGGCTTTCCGGAt                         <  1:1844843/76‑1 (MQ=255)
                                                    ccGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGAtt                        <  1:3740175/76‑1 (MQ=255)
                                                    ccGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGAtt                        <  1:5777061/76‑1 (MQ=255)
                                                     cGATGTGGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTa                       <  2:1294422/76‑1 (MQ=255)
                                                     cGATGCCGTTCGGTTTC‑TTCCCCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTa                       <  2:1504027/76‑1 (MQ=255)
                                                     cGATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGGTTa                       >  2:730954/1‑76 (MQ=255)
                                                      gATGACGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTaa                      >  2:328648/1‑76 (MQ=255)
                                                       gtGACTTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCCCCCCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAAc                     <  1:8453815/75‑1 (MQ=255)
                                                        tgccGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAAcc                    <  1:1628365/73‑1 (MQ=255)
                                                         gACGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCt                   <  2:5869564/76‑1 (MQ=255)
                                                           cGTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGc                 >  1:2113058/1‑76 (MQ=255)
                                                           cGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGc                 <  1:2170121/76‑1 (MQ=255)
                                                           cGTTCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGc                 <  1:4100632/76‑1 (MQ=255)
                                                            gTTCAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCa                <  2:7903686/76‑1 (MQ=255)
                                                              tCAGTTTCTTTC‑CCCCCCCCCCCGTGATCACTCCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAAt              <  1:5553730/76‑1 (MQ=255)
                                                              tCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAAt              <  2:2580648/76‑1 (MQ=255)
                                                              tCAGTATCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAAt              <  1:6100999/76‑1 (MQ=255)
                                                               cAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCTCGCCACACGTGCTGTACTGGCTTTCCGGCTTAACCTGCAATg             >  2:6739244/1‑76 (MQ=255)
                                                               cAGTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATg             >  1:3332894/1‑76 (MQ=255)
                                                                aGTATCTTTCCCCCCCCCCCTCGTGATCACCCCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATg             <  2:353477/76‑1 (MQ=255)
                                                                 gTATCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGAc           >  1:8575764/1‑76 (MQ=255)
                                                                   aTCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGGTTAACCTGCAATGACga         >  1:6860230/1‑76 (MQ=255)
                                                                   aTCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACga         >  2:6154560/1‑76 (MQ=255)
                                                                   aTCTTTCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACga         >  1:2624432/1‑76 (MQ=255)
                                                                    tCTTTC‑CCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACgag        <  1:2479165/76‑1 (MQ=255)
                                                                      tttCT‑CCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGaa      <  2:4544048/76‑1 (MQ=255)
                                                                           cccccccccTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTc  <  1:7986387/76‑1 (MQ=255)
                                                                           |                                                                           
TTTTGAAGGCTGGCAGCAACGCTGGCGACACGACTCCAGTACCTTAAACTGCCCGATGACGTTCAGTATCTTTCTCCCCCCCCCTCGTGATCACACCCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTC  >  REL606/2195174‑2195324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑