| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,759,444 | 0 | A | C | 35.7% | 327.4 / 29.7 | 154 | H261P (CAC→CCC) | ydiS | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base A (89/10); new base C (0/55); total (89/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-32 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 210 total aligned reads displayed.
GCTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGTC > REL606/1759370‑1759518 | gCTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAacac < 1:1922291/76‑1 (MQ=255)gCTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAacac < 2:203840/76‑1 (MQ=255) cTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACAcc < 2:754334/76‑1 (MQ=255) cTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACAcc < 1:7514461/76‑1 (MQ=255) cTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGAGCCGCAAATGCTGGAAGATTTTAAACAACAcc < 1:4228962/76‑1 (MQ=255) gTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCg > 2:8274683/1‑76 (MQ=255) gTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCg > 1:1602521/1‑76 (MQ=255) ttGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGcc > 1:2349094/1‑76 (MQ=255) tgtgGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCa > 2:7320913/1‑76 (MQ=255) ggATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTc < 1:1545586/76‑1 (MQ=255) gATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCg > 1:8687707/1‑76 (MQ=255) gATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCg > 1:1999460/1‑76 (MQ=255) gATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCg > 1:3453060/1‑76 (MQ=255) gATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACACCGCCATTCg > 2:8518638/1‑76 (MQ=255) gATTGGCTGAAATCGCCCATGCGCAAAAAAGCGTGCCGAAAATGCAGAAAGATTTAAAACAACCCCCCGCCATTCg < 2:5905520/76‑1 (MQ=255) aTTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGc > 1:4682352/1‑76 (MQ=255) ttGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGcc > 1:5749840/1‑76 (MQ=255) gggATATCGCCCATGCCCAAAAAAGCGTGCCGCAAATGCTGGAAAATTTTAAACAACACCCCGCCATTCGCCCGCt < 2:6494154/74‑1 (MQ=38) tGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTg > 1:6914568/1‑76 (MQ=255) atatCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGAt > 2:3499471/1‑76 (MQ=255) tatCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGAtt > 1:5725296/1‑76 (MQ=255) atCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGGTTa > 2:1371170/1‑76 (MQ=255) atCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTa > 2:7443065/1‑76 (MQ=255) gCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAg‑cg > 1:3169232/1‑76 (MQ=255) gCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAg‑cg > 2:8791026/1‑76 (MQ=255) gCCAATGCGCAAAAAAGCGTGCCGCAAAGGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAg‑cg < 1:3604890/76‑1 (MQ=255) cccATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAg‑cgg < 2:4288063/76‑1 (MQ=255) cccATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAg‑cgg > 2:3729540/1‑76 (MQ=255) ccATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAg‑cggc > 2:5553389/1‑76 (MQ=255) ccATGCGAAAAAAAGCGTGCCGCAAATGTTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAg‑cggc < 1:3036807/76‑1 (MQ=38) ccATGCGAAAAAAAGCGTGCCGCAAATGCGGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAg‑cggc < 2:5261365/76‑1 (MQ=37) cATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAg‑cggcg > 1:5982531/1‑76 (MQ=255) cATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAg‑cggcg > 1:6718264/1‑76 (MQ=255) tGCGAAAAAAAGCGTGCCGCAAATGCGGAAACATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑Cggcggc < 1:5063562/76‑1 (MQ=38) gcgcAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCa < 2:8705724/76‑1 (MQ=255) gcgaAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCa < 1:8158463/76‑1 (MQ=37) cgaaaaaaaGCGTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCaa < 1:7255208/73‑1 (MQ=255) gaaaaaaaGCTTGCCCAAAATGCGGAAAGATTTAAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCaaa < 1:6573092/74‑1 (MQ=255) caaaaaagGCTTGCCGAAAATGCTGAAAGATTTAAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCaaa < 2:1105378/74‑1 (MQ=255) cAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAAc < 2:4482905/76‑1 (MQ=255) aaaaagGCGTGCCGCAAATGCTGGAAGATTTAAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACt < 2:6160959/76‑1 (MQ=38) aaaaaaGGGTGCCGCAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACt < 2:8039708/76‑1 (MQ=38) aaaaaaGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGGaaaaa > 2:641692/1‑74 (MQ=255) aaaaaGCGTGCCGCAAATGCTGGAAGTTTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTg < 1:4379085/76‑1 (MQ=255) aaaaaGCGTGCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTg < 1:4448739/76‑1 (MQ=255) aaaaaGCGTGCCGCAAAGGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTg < 2:2556107/76‑1 (MQ=255) aaaaaGCGTGCCGAAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTg < 2:5890540/76‑1 (MQ=255) aaaaGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGGAAAATGc > 2:5085778/1‑76 (MQ=255) aaaaGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGc > 2:6913405/1‑76 (MQ=255) aaaaGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAAATGc > 2:1853217/1‑76 (MQ=255) aaaGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGGAAACAGCt > 2:7217161/1‑76 (MQ=255) aaaGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCGAACAGcc > 1:5468354/1‑75 (MQ=255) aggCGTGCCGAAAATGCTGAAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCtt < 1:799142/74‑1 (MQ=255) aaGCGTGCCGCAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCtt < 2:2603608/76‑1 (MQ=255) aaGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCtt > 1:3905866/1‑76 (MQ=255) aaGCGTGCCGCAAATGCTGAAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCtt < 2:5383905/76‑1 (MQ=255) agggTGCCGCAAATGCTGGAAGATTTAAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTg < 2:5758575/73‑1 (MQ=38) aGCGTGCCGCAAATACTGGAAGTTTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTg < 2:4214352/76‑1 (MQ=255) aGCGTGCCGAAAATGCTGGAAGATTTTAACCAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTg < 2:7762430/76‑1 (MQ=255) aGCGTGCCGAAAATGCTGGAACATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTg < 2:4451504/76‑1 (MQ=255) gCGTGCCGCAAATGCTGGAAGATTTTAAACACCACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGa < 2:9002757/76‑1 (MQ=255) cGTGCCGCAAATGTGGGAATGTTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGaa < 1:4786838/76‑1 (MQ=255) cGTGCCGCAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGaa < 1:8930661/76‑1 (MQ=255) gTGCCGCAAATGCTGGAAGATTTAAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAat < 1:6403310/76‑1 (MQ=255) tGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAAACGTTTGAata > 1:583986/1‑76 (MQ=38) tGCCGAAAATGCTGAAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAata < 2:7290919/76‑1 (MQ=255) tGCCGAAAATGCGGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAata < 2:891805/76‑1 (MQ=255) gCCGCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAatat < 2:3528811/76‑1 (MQ=255) gCCGAAAATGCTGGAAGATTTTAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAatat < 1:3765790/76‑1 (MQ=39) ccGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGGAAAATGCTTTAATAtt > 2:344631/1‑76 (MQ=38) cGCAAATGCTGAAAGATTTAAAACACCCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTc < 1:311581/76‑1 (MQ=255) gCAAATGCTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTcc < 2:4746806/76‑1 (MQ=255) aaaTGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAAAATTTcgc > 1:2594349/1‑76 (MQ=255) aaaTGCTGAAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCcgc < 2:4616042/76‑1 (MQ=255) aTGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGGAAAATGCTTGAATATTCCgcgc > 2:7597938/1‑76 (MQ=255) aTGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTTAACATTCCgcgc > 1:2554863/1‑76 (MQ=255) cTGGAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCata < 2:1602813/76‑1 (MQ=255) tgaaaGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCatat < 1:4016377/73‑1 (MQ=255) ggATGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGGAAACTGCTTGAATATTTCGCGCATATg > 2:7249278/1‑76 (MQ=255) ggAAGATTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATg < 2:569809/76‑1 (MQ=255) ggAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATg > 2:94514/1‑76 (MQ=255) gAAGTTTTTAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATgg < 2:8451940/76‑1 (MQ=255) aaaGATTTTAACCAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATgg < 2:7312380/75‑1 (MQ=255) aaGATTTAAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGt < 2:1268747/76‑1 (MQ=255) aaGATTTAAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGt < 1:1467590/76‑1 (MQ=255) aTTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCCTATGGTGcc > 1:865031/1‑76 (MQ=255) aTTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGcc > 1:238921/1‑76 (MQ=255) ttAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTTCCCGCATATGGTGcccgg > 2:718787/1‑73 (MQ=255) ttAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGa > 2:704952/1‑76 (MQ=255) ttAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAAAAGCTTGAATATTCCGCGCATATGGGGCCGGa > 2:6506904/1‑76 (MQ=255) tAAACAACCCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGaa < 1:5411961/76‑1 (MQ=255) tAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGaa > 2:6703616/1‑76 (MQ=255) acaacaCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGc > 1:6960684/1‑76 (MQ=255) caacaCCCCGCCATTCGCCCGCTGATTAG‑CGGCGGGAAACCGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCg > 2:517773/1‑76 (MQ=255) aaccCCCCGCCATTCGGCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCgg < 2:1997530/76‑1 (MQ=255) acacCCCGCCATTCGCCGGC‑GGTTAGCCGGCGGCAAACCGCTTGAAAATTCCCCGCAATTGGTGCCGGAAGggggg > 2:4893002/1‑75 (MQ=255) acacCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCCAAATGCTTGAATATTCCGCGCATATGGTGCCGGAAGggggg > 2:2457140/1‑75 (MQ=255) acacCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGt > 1:2163184/1‑76 (MQ=255) acacCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACAGCTTGAATATTCCGCGCAAATGGTGCCGGAAGGCGGt > 1:6199809/1‑76 (MQ=255) cacCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGgggggc > 1:7178781/1‑74 (MQ=255) | GCTGGTTTGTGGATTGGGTGATATCGCCCATGCGCAAAAAAGCGTGCCGCAAATGCTGGAAGATTTTAAACAACACCCCGCCATTCGCCCGCTGATTAG‑CGGCGGCAAACTGCTTGAATATTCCGCGCATATGGTGCCGGAAGGCGGTC > REL606/1759370‑1759518 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |