| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,213,003 | 0 | A | C | 44.2% | 141.9 / 26.1 | 104 | V184G (GTG→GGG) | fruK | 1‑phosphofructokinase |
| Reads supporting (aligned to +/- strand): ref base A (38/20); new base C (0/46); total (38/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.26e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 204 total aligned reads displayed.
GCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGAC > REL606/2212928‑2213078 | gcAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCa < 2:2855497/76‑1 (MQ=255) cAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGGTAGGGTTCAc > 1:4147178/1‑76 (MQ=255) gCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCa < 1:7745876/76‑1 (MQ=255) cTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAg < 1:8182768/76‑1 (MQ=255) cTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGGTAGGGTTCACCAg > 2:6697308/1‑76 (MQ=255) ttCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGGTTCACCacc > 1:260232/1‑74 (MQ=255) ttCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGGTAGGGTTTACCCGc > 1:5164813/1‑76 (MQ=255) ttCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCCGCGGGTTAGG‑GTCACCAGc > 2:2723193/1‑76 (MQ=255) ttCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTACCCAGATTTCCAGCTCGCGGC‑GGGTAGGGTTCAccccc > 1:6375530/1‑73 (MQ=255) cAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCa < 1:8118273/76‑1 (MQ=255) aaccacaTCTTTCATTGCAGGCAGTTTCCGGCCTGCCCAGATTCCCAGCTCGCGGC‑GGTTGGTTTCCCCCAGCCAc < 2:3747645/73‑1 (MQ=255) cacaTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGGTAGGGTTTACCCGCCACggg > 1:2517063/1‑75 (MQ=255) cacaTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCCCGCGGC‑GGTTAGGTTTCCCCAGCCACGGt < 2:3506690/76‑1 (MQ=255) acaTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTg < 2:1610532/76‑1 (MQ=255) caTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGc < 2:3065745/76‑1 (MQ=255) aTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGcc < 2:8570979/76‑1 (MQ=255) aTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGGTTCACCAGCCCCCGTggc > 1:535368/1‑74 (MQ=255) tCTTTCATTTCGGGCAGTTTACGGCCTGCCCATATTTCCAGGTCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCg < 2:5039648/76‑1 (MQ=255) cTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGGTAGGTTTTACCTGCCCCCGTGCCGc > 2:5958777/1‑76 (MQ=255) tttcatttcaGGCAGTTTACGGCCTGCCCAGTTTTCCAGCGCGCGGC‑GTTTAGCTTTCCCCAGCCACGGTTCCGC‑t < 2:749517/76‑1 (MQ=255) ttcatttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGGGTAGGTTT‑TCCAGCCACGGcgccgcgt > 2:2343720/1‑74 (MQ=255) catttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTc < 1:8206946/76‑1 (MQ=255) catttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTc < 1:5090029/76‑1 (MQ=255) cattccaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTc < 1:5374429/76‑1 (MQ=255) atttcaGGCAGTTTCCGGCCTGCCCAGATTCCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑tttgg < 2:4855371/76‑3 (MQ=255) atttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGGTTCACCCACCCCGGGGGCGC‑tttta > 1:2728336/1‑74 (MQ=255) atttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGGTAGGGTTTACCAGCCACGGGGCCCC‑CTTCa > 1:3238097/1‑76 (MQ=255) ttgaGGCAGTTTACGGCCTGCCCAGATGTCCAGCTCGGGGC‑GGTTGGGTTTCACCAGCCACGGTGCCGC‑TTTCaaa < 2:8962101/73‑1 (MQ=255) tcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAAc < 2:1742057/76‑1 (MQ=255) caGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGGTAAGGTTACCCCA‑CCCCCGTGCCGCTTTTCAAAc > 1:8434317/1‑76 (MQ=255) caGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAAcc < 1:1410509/76‑1 (MQ=255) aGGCAGTTTCCGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCt < 1:387921/76‑1 (MQ=255) gCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGGTAGGGTTCACCAGCCACGGGGCCGC‑TTTCAAAACTGc > 2:8661551/1‑76 (MQ=255) attttACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGcta < 1:7920546/74‑1 (MQ=255) aGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGGTTGGGTTTACCCACCAACGG‑GGCGC‑CTTTAAACCTTctt > 2:836848/1‑75 (MQ=255) aGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTTCCGC‑TTTCAAACCTGcta < 2:4877168/76‑1 (MQ=255) gTTTCCGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGctac < 1:7330467/76‑1 (MQ=255) tttCCGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGTTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGctact < 1:1247873/76‑1 (MQ=255) tttCCGGCCTGCCAAGTTTCCCAGCTCGGGGG‑GTTTAGTTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGctact < 1:7405133/76‑1 (MQ=255) tttAGGGCCTCCCCAGTTTTCCAGCCCGCGGG‑GTTTGGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGctact < 1:3441288/76‑1 (MQ=255) tttACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTTACCCGGCACGGGGCCGC‑TTTCAAACCTGccact > 1:3745401/1‑76 (MQ=255) ttcaCGGCCGCCCCAGTTTCCCAGCTCGCGCC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGctact < 1:4943727/73‑1 (MQ=255) ttACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGGTAGGGTTTACCAGCCCCCGGGCCGC‑TTTTAAACCCTctacta > 2:8262547/1‑76 (MQ=255) tACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GTTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTaa < 2:3570305/76‑1 (MQ=255) tACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTaa < 2:6028123/76‑1 (MQ=255) tcGGCCTGCCAAAACTTCCAGCCCGCGGC‑GATTAGGTTCCACCAGCCACGGTTCCGC‑TTTCAAACCTGCTACTAAc < 1:6764537/75‑1 (MQ=255) aCGGACTGCCCAGATTTCCAGCCCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAAc < 1:2231723/76‑1 (MQ=255) cGGCCTCCCCGTATTTCCAGCTCGCGGG‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAAca < 2:3979642/76‑2 (MQ=255) ggCCTGCCCAGATTTCCAGCTCGCGGC‑GGGTTGGTTTCACCAGCCACGGGGCCGC‑TTTCAAAACTTCTACTTACGc > 2:6954817/1‑76 (MQ=255) ggCCTCCCCAAGTTTCCAGCTCGCGGC‑GGTTAGGTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGc < 2:3245110/76‑1 (MQ=255) ggCCTCCCAAGATTACCAGCCCCCGGG‑GGTTAGTTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGc < 2:146953/76‑1 (MQ=255) gCCGGCCCAGATTCCCAGCTCGCGGC‑GTTAAGGTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCt < 2:3708281/76‑1 (MQ=255) ccTGGCCAGATTTCCAGCTCGCGGC‑GGTTAGGGTTCACCAGCCACGGGGCCGC‑TTTCAAACCTGCTACTAACGCtt > 1:4535986/1‑76 (MQ=255) ccTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGTTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCtt < 1:8739108/76‑1 (MQ=255) cggCCCAGATTCCCAGCTCGCGGC‑GGTTAGTTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTc < 2:7777631/74‑1 (MQ=255) cgccccAGTTTCCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTc < 1:771603/73‑1 (MQ=255) tccccAGATTCCCAGCTCGCGGC‑GGTTGGTTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCa < 1:8707762/74‑1 (MQ=255) gCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCACGGGGCCGC‑TTTCAAACCTGCTACTAACGCTTCAc > 1:880661/1‑76 (MQ=255) gCCCAGATTTCCAGCTCGCGGC‑GGGTAGGGTTCACCAGCCACCGTGGCGC‑TTTCAAACCCGCTACTAACGGCTCAc > 2:3470145/1‑76 (MQ=255) cccAGATTCCCAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTTCCGC‑TTTCAAACCTGCTACTAACGCTTCACg < 2:5766437/76‑1 (MQ=255) aGATTCCCGGCTCGGGGC‑GGTTGGGTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGct < 2:2977137/76‑1 (MQ=255) gATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGcta > 2:2189133/1‑76 (MQ=255) gATTCCCGGCCCGCGGG‑GTTTAGTTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGcta < 2:318404/76‑1 (MQ=255) aaTTTCCACCTCGCGGC‑GTTAAGGTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGcta < 2:7373281/75‑1 (MQ=255) aTTTCCAGCTCGCGGC‑GGGTAGGTTTTTCCAGCCACGGTGCCGC‑TTTCAAACCCGCTACTAACGCTTCACCGctat > 1:748614/1‑75 (MQ=255) ttcccAGCTCGCGGC‑GTTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGctact < 2:5526746/73‑1 (MQ=255) ttCCAGCTCGCGGC‑GGGTAGGGTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCCCAGctacta > 2:7537651/1‑76 (MQ=255) tCCAGCTCGCGGC‑GTTTAGTTTTCCCCAGCCACGGTTCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTAt < 1:1485929/76‑1 (MQ=255) tCCAGCTCGCGGC‑GTTTAGTTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTAt < 1:4960113/76‑1 (MQ=255) cggCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCa < 1:284663/74‑1 (MQ=255) cAGCTCGCGGC‑GGTTAGTTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCa < 1:6159672/76‑1 (MQ=255) cAGCTCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCa < 2:1141239/76‑1 (MQ=255) cAGCCCGCGGC‑GGTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCa < 2:6617254/76‑1 (MQ=255) aGCTCGCGGC‑GTTTGGGTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCaa < 2:6177334/76‑1 (MQ=255) gcccgcgGC‑GGTTAGTTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCaaa < 1:8248577/73‑1 (MQ=255) ctggcggc‑gGTTGGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAg < 1:4411501/73‑1 (MQ=255) cccgcgGC‑GTTTAGTTTTCCCCAGCCACGGTTCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAg < 2:2006981/74‑1 (MQ=255) ccgcgGC‑GTTTAGGTTCCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGa < 1:986732/75‑1 (MQ=255) gggggc‑ggTGAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGAt < 2:46005/73‑1 (MQ=255) gcggc‑gGTTGGTTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATa < 2:2582674/76‑1 (MQ=255) gggc‑ggTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATaa < 1:1459690/75‑1 (MQ=255) gggc‑ggTTAGGTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATaa < 2:5269337/75‑1 (MQ=255) ggc‑ggTTAGGTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAAt < 1:2382653/76‑1 (MQ=255) c‑ggTTAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGc > 2:6273793/1‑76 (MQ=255) ggTTAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCa > 1:3114345/1‑76 (MQ=255) ttAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAg > 2:1298738/1‑76 (MQ=255) ttAGGTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAg < 1:6487229/76‑1 (MQ=255) ttAGGTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAg < 2:1913121/76‑1 (MQ=255) tgggTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAgg < 1:5677175/74‑1 (MQ=255) tAGTTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAgg < 1:8595034/76‑1 (MQ=255) tAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCaaag > 2:3827875/1‑74 (MQ=255) gggTTCCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGa < 2:7979805/75‑1 (MQ=255) gTTTCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGaca < 2:4246415/76‑1 (MQ=255) gTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGaca > 1:5984775/1‑76 (MQ=255) tttCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGacac < 1:8522296/76‑1 (MQ=255) tttCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGacac > 1:1904144/1‑76 (MQ=255) ttCCCCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACt < 1:4373440/76‑1 (MQ=255) ttCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGAGACt > 2:281153/1‑76 (MQ=255) ttCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACt < 1:2196213/76‑1 (MQ=255) tCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTg > 1:5271543/1‑76 (MQ=255) | GCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGC‑GGTTAGGTTTCACCAGCCACGGTGCCGC‑TTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGAC > REL606/2212928‑2213078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |