| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,515,909 | 0 | A | C | 46.9% | 80.8 / 54.9 | 81 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (36/7); new base C (0/38); total (36/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-16 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 116 total aligned reads displayed.
TTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT > REL606/2515835‑2515984 | ttAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGGGGGTTCGTCAtccac > 2:907098/1‑76 (MQ=255)ttAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGaccac < 1:6139776/76‑1 (MQ=255) tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGaccacc < 2:4207724/76‑1 (MQ=255) tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGaccacc < 1:7704569/76‑1 (MQ=255) tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGaccacc < 2:8286695/76‑1 (MQ=255) tAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGaccaca < 2:2944203/76‑2 (MQ=255) acaacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAcc‑ag < 2:6005470/76‑1 (MQ=255) aacGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAcc‑agcc < 1:1028872/76‑1 (MQ=255) acGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTTCTCGACCAcc‑cgccc > 1:6471315/1‑75 (MQ=255) acGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAcc‑agcca < 1:6030807/76‑1 (MQ=255) cGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCAcc‑agccag < 1:2391513/76‑1 (MQ=255) tggAGGAAAACGCGATCCCGGTGAATGCAACAAGGGCGCGGGTATGGCTGCGGCTTCACCGCCCCCC‑AGCCAGTCg < 2:6118372/74‑1 (MQ=255) ggaaaacCGCGATCCGAGTTGATGCAACAATGGCGGGGTTATGGCTGCGGCTTCATCGACCCCC‑AGCCAGTCGGCt < 2:4232168/73‑1 (MQ=255) ggAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCCTTCTCGACCCCCAACCCAGCCGGCt > 1:7819795/1‑76 (MQ=255) gaaaacCGCAATCCCAGTTGATGCAAAAAGGGCGCGGGTAGGGCTGCGGCTTCACCGCCCCCC‑AGCCAGTCGGCtt < 2:2323018/74‑1 (MQ=255) gaaaaaCGCGATCCCAGTTGATGCAACAATGGCGCGGTTATGGCTGCGGCTTCATCGACCCCC‑AGCCAGTCGGCtt < 2:2018603/74‑1 (MQ=255) gaaaaaCGCGATCCCAGTTGATGCAACAATGGCGCAGGTAGGGCTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCtt < 2:6417115/74‑1 (MQ=255) gAAAGCGCGATACCAGTTGATGCAACAAGGGCGCAGGTATGGCTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTg < 2:3128735/76‑1 (MQ=255) gAAACCGCGATACCAGTTGATGCAACAATGGCGCAGGTAGGGCTGCGGCTTCATCGACCCCC‑AGCCAGTCGGCTTg < 2:7275386/76‑1 (MQ=255) aaaaaCGCAATACCAGTTGATGCAAAAAGGGCGCAGGTAGGGCTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTg < 2:3466807/75‑1 (MQ=255) ggaaTACCGGTTGATGAAAAATGGGCGCAGTTAGGGCTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATc < 2:1164847/73‑1 (MQ=255) ttccATTTGGTGAAACAATGGCGAGGGATTGGTTGCGGCTTCATCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTg < 1:2127499/74‑1 (MQ=255) tcccAGTTGATCCAAAAAGGCGGCAGTTTTGGCGGCGGCTCCATCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTg < 1:8579370/74‑1 (MQ=255) tcccAGTTGAGGAAACAGGGGCGCAGGTTGGGCTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTg < 2:3612052/74‑1 (MQ=255) tACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTTATCCGCCACC‑AGCCCGGCGGCTTTCT‑CCTCGccg > 1:1174681/1‑74 (MQ=255) aGTTGATGCAACAATGGCGCAGGTATGGCTGCGCCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTa < 1:5559565/76‑1 (MQ=255) gtGATGCAACAAGGGCGCAGGTAGGGCTGCGCCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAAc < 1:538790/75‑1 (MQ=255) tgatgaAACAAGGGCGCAGGTAGGGCTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACa < 1:2441387/76‑1 (MQ=255) tGATGCAACAATGGCGCGGGTATGGCTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACa < 1:5029001/76‑1 (MQ=255) tGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACa < 1:6021615/76‑1 (MQ=255) tGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACC‑AGCCAGGCGGCTTGGT‑CCTCGCTGGGTaaaa > 1:6679851/1‑74 (MQ=255) tGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCACCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACa < 1:2124655/76‑1 (MQ=255) tGATGCAACAAGGGCGCGGGTAGGGCTGCGGCTTCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACa < 1:1072201/76‑1 (MQ=255) tGATCCAACAATGGCGCAGGTTTGGCTGCGGCTTCCTCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACa < 1:6296881/76‑1 (MQ=255) gATGCAACAAGGGCCGAGGTATGGCTGCGGCTTCATCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACaa < 1:7206225/76‑1 (MQ=255) aacagTGGCGCGGGTAGGCCTGCGGCTCCACCCCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCAt < 1:1899258/76‑1 (MQ=255) aacaaGGGCGCAGGTATGGTGGCGGCGTCAACGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCAt < 2:1984807/76‑1 (MQ=255) aacaaGGGCGCAGGTATGGCTGCGGTTTCATCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCAt < 1:8370546/76‑1 (MQ=255) acgaTGGCCCGGGTAGGGCTGCGCTTTCATCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATc < 1:6563331/73‑1 (MQ=255) aggggCGCGGGTGTGCCTGCGGCTTCACCCCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCg < 2:965746/73‑1 (MQ=255) aggggCGCGGGTAGGCCTGCGCTTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCg < 1:5370771/73‑1 (MQ=255) agggCGCAGTTATGCCTGCGTCTTCATCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCgg < 1:4192746/74‑1 (MQ=255) agggCGCAGGTGTGGCTGCGGCTTCACCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCgg < 2:4977683/74‑1 (MQ=255) aTGGCGCGGGTTTGGTGGCGCTTTCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCgg < 1:6857560/76‑1 (MQ=255) ggCGCGGGTAGGGCGGCGGTTTCATCGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGgcg < 2:2125628/76‑1 (MQ=255) cgcgGGTAGGCCTGCGCCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCa < 2:3509379/76‑1 (MQ=255) ggTAGGGCGGCGGCTCCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATaa < 1:5093467/76‑1 (MQ=255) gTATGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAAGCATCCGGGGCAATaaa > 1:5979059/1‑76 (MQ=255) gTATGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAAAAAAGGACCCGGCGCAATaaa > 1:4071282/1‑76 (MQ=255) gTATGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCCGGCTAACAAGGCATCCGGCGCCAaaaa > 1:2627133/1‑76 (MQ=255) gTATGGCGGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATaaa < 2:774952/76‑1 (MQ=255) tacggCTGCTGCTTCATCGCCCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAg < 2:3965834/73‑1 (MQ=255) ggggCTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGc < 1:1110462/74‑1 (MQ=255) agggCTGCGGCTCCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGc < 1:5938803/74‑1 (MQ=255) aTGGCTGCGGCTTTATCGACCACC‑AGCCCGTCGGCTTGCTCCTTCCCTGGTTAAAAAGGAACCCGGCGCaaaaaac > 2:354703/1‑75 (MQ=255) aTGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGGTAACAAAGCATCCGGCGCAATaaaac > 1:1278573/1‑74 (MQ=255) aTGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAAGCAACCCGCGCCAaaaaac > 2:4189076/1‑74 (MQ=255) aTGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCCGGGTAACAAAGCATCCCGCGCAATAAAGc > 1:7774697/1‑76 (MQ=255) tGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGGTGGCTAAAAAAGCATCCCGGGCAATAAAACg > 2:5953936/1‑76 (MQ=255) tGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGGAACCGGGGCAATAAAGCg > 1:6237195/1‑76 (MQ=255) tGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCCTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAAAAAAGCg > 2:6076962/1‑76 (MQ=255) gggCTGCGGCTCCATCCCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCg < 1:6579320/75‑1 (MQ=255) ggCTGCGGTTCCATCCCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCCAACAAGGCATCCGGCGCAATAAAGCGa < 2:229783/76‑1 (MQ=255) ggCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTTCT‑TATCCCCGGCTAACAAAGCATCCGGGGCAATAAAACGa > 2:5225662/1‑76 (MQ=255) gcggCGGCTCCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaa < 1:8530686/73‑1 (MQ=255) gCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCCATAAAGCGaa > 1:1911891/1‑76 (MQ=255) gtGCGGTTTCTTCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaaa < 2:8653197/75‑1 (MQ=255) cggCGGCTCCACCCCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaaa < 1:149025/74‑1 (MQ=255) cTGCGGCTTCATCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaaa < 2:7307560/76‑1 (MQ=255) atGCGCCTTCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGaaa < 1:990309/75‑1 (MQ=255) tGCCGCTTCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAAc < 1:5321752/76‑1 (MQ=255) gctgCTTCACCGCCCCCC‑AGCCAGTCGGCTTACT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACt < 2:1409504/73‑1 (MQ=255) gCGGCTTTATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCCTGCTAAAAAGGCATCCGGGGCCAAAAAACGAAAca > 2:6010143/1‑75 (MQ=255) cGGCTTCATCGACCACC‑AGCCAGTCGGCTTTCT‑CATCGCTGGGTAACAAAGCAACCGGCGCAAAAAAGCGAAAAtt > 2:7715182/1‑76 (MQ=255) ggCTTCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtc < 1:4400387/76‑1 (MQ=255) cTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtctc > 1:6542902/1‑76 (MQ=255) cTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtctc > 1:736945/1‑76 (MQ=255) cTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtctc > 1:3344224/1‑76 (MQ=255) cTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtctc > 1:7702392/1‑76 (MQ=255) cTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtctc > 1:8227930/1‑76 (MQ=255) cTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCCGCGCAATAAAGCGAAACTtctc > 1:180023/1‑76 (MQ=255) cTTCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTtctc < 2:4947829/76‑1 (MQ=255) ttCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg > 1:4700527/1‑76 (MQ=255) ttCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg > 1:6047791/1‑76 (MQ=255) ttCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg > 1:2166250/1‑76 (MQ=255) ttCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg > 1:4820429/1‑76 (MQ=255) ttCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg > 1:1410416/1‑76 (MQ=255) ttCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg > 2:697888/1‑76 (MQ=255) ttCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAAATTCTCg > 1:6438786/1‑76 (MQ=255) ttCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg < 1:3515270/76‑1 (MQ=255) ttCACCGCCCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCg < 1:5665852/76‑1 (MQ=255) tCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCCATAAAGCGAAACTtctccc > 1:2795427/1‑74 (MQ=255) tCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCCGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGc > 2:7763657/1‑76 (MQ=255) aTCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGccc > 2:5141671/1‑76 (MQ=255) tCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCg > 1:4687559/1‑76 (MQ=255) cGACCCCC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGc < 2:839432/76‑1 (MQ=255) gACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCa > 1:4678886/1‑76 (MQ=255) gACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCa > 2:825622/1‑76 (MQ=255) gACCACC‑AGCCAGTCGGCTTGCC‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCa > 2:1716339/1‑76 (MQ=255) acc‑aGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAAtt > 1:6472120/1‑76 (MQ=255) | TTAACAACGTTCGAGGAAAACGCGATACCAGTTGATGCAACAATGGCGCAGGTATGGCTGCGGCTTCATCGACCACC‑AGCCAGTCGGCTTGCT‑CATCGCTGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATT > REL606/2515835‑2515984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |