| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,472,700 | 0 | C | G | 36.6% | 106.6 / 86.8 | 101 | A290G (GCG→GGG) | yhgA | predicted transposase |
| Reads supporting (aligned to +/- strand): ref base C (50/14); new base G (1/36); total (51/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.17e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 193 total aligned reads displayed.
TAATGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAA > REL606/3472625‑3472775 | taatGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTgcggc < 1:3884057/76‑1 (MQ=255) aatGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTgcggcg > 2:168888/1‑76 (MQ=255) aatGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTgcggcg < 2:8411803/76‑1 (MQ=255) tGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAg < 2:4947742/76‑1 (MQ=255) tGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAg > 2:7534402/1‑76 (MQ=255) tGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAg < 1:3698433/76‑1 (MQ=255) gCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGc > 2:1006256/1‑76 (MQ=255) cTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAgac > 2:15981/1‑74 (MQ=255) cTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGcc > 1:6392923/1‑76 (MQ=255) cTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGcc < 2:50345/76‑1 (MQ=255) ttGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCa < 1:8781768/76‑1 (MQ=255) ttGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCa < 1:5628915/76‑1 (MQ=255) ttGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGACa > 2:5894878/1‑76 (MQ=255) tGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGCGGCAg > 1:2613194/1‑76 (MQ=255) tGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAg < 1:4488635/76‑1 (MQ=255) tGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAg < 2:8073675/76‑1 (MQ=255) tGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAg > 1:4763354/1‑76 (MQ=255) aaTCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTa > 2:448491/1‑76 (MQ=255) ccGGAGTCCCTCTTACAGACATCATGCGCTTTCCGGGGGTGTCAGAAGAAGAGTTGGCTGGGGGGAGCCAGTAAAt < 2:5200352/76‑1 (MQ=255) ccGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAAt > 1:7723905/1‑76 (MQ=255) agccccTCTAACAGACACCATGCGCTTTCCGGGGGTGTAAGAAGAAGGGTGGGCGGCGGGGAGCCAGTAAATTTCt < 2:3287147/73‑1 (MQ=255) aGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAAATTCt > 1:6837315/1‑76 (MQ=255) aGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGGAAATTTCt > 2:376215/1‑76 (MQ=255) cccTCTTACAGACATCATGCGCTTTACGGGGGTGTCAGAAGAAGAGTTGGCGGGGGGGAGCCAGTAAATTTCTGtc < 2:5378323/76‑1 (MQ=255) cccTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGCGGCAGTAAAATTCtttt > 2:7029074/1‑73 (MQ=255) ccTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGCACCCGTAAATTTCTGtct > 1:7212602/1‑76 (MQ=255) ccTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAACAAATTTCTGgct > 1:8947612/1‑76 (MQ=255) ctctTACAGACATCATGCTCTTTACGGGGGTCTGAAAAGAAGGGTGGGCTGCGGGGAGCCAGTAAATTTCTGtctc < 1:3566996/76‑1 (MQ=255) ctctTACAGACATCATGCGCTTTCCGGGGTTGTCAGAAGAAGAGTTGGCGGGGGGGAGCCAGTAAATTTCTGtctc < 2:4763354/76‑1 (MQ=255) ctctTACAGACATCAGGCGTTTTACGGGGGTGTAAAAAGAAGAGTTGGCGGCGGGGAGCCAGTAAATTTCTGtctc < 2:5406753/76‑1 (MQ=255) tctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAAATTTTGtctcc > 2:21451/1‑75 (MQ=255) tctAACAGACATCAGCCGCTTTCCGGGGGTGTAAGAAGAAGAGTTGGCGGCGGGGAGCCAGTAAATTTCTGTCTCg < 2:5917845/76‑1 (MQ=255) ctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGc > 2:6834038/1‑76 (MQ=255) ctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGc > 1:8841246/1‑76 (MQ=255) ctTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGGAAATTTCTGTCTCGc > 1:3524358/1‑76 (MQ=255) ttACAGACATGAGGCGCTTTCCGGGGGTTTAAGAAGAGGAGTGGGCTGGGGGGAGCCAGTAAATTTCTGTCTCGcc < 2:364267/76‑1 (MQ=255) ttACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGcc > 2:850850/1‑76 (MQ=255) ttACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGGAAATTTTTGTCTCGcc > 2:5654540/1‑76 (MQ=255) tACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGAGGCGAGCCAGTAAATTTTTCTATCGCCa > 2:3820107/1‑76 (MQ=255) cAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGGCAGGAAATTTCTGTCTCGCCAtt > 2:8585319/1‑76 (MQ=255) cAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCCGTAAAATTCTGTCTCCGCAtt > 1:1138041/1‑76 (MQ=255) gaaCATCGGGCGCTTTACGGGGGGGTAAGAAGAGGGGTGGGCGGCGGGGAGCCAGTAAATTTCTGTCTCGCCAttt < 2:1457584/74‑1 (MQ=255) aGACATCATGCGCTTTCGGGGGGTGTAAGAAGAGGAGTGGGCTGGGGGGAGCCAGTAAATTTCTGTCTCGCCAttt < 1:3213397/76‑1 (MQ=255) aGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGGGGCGGGCCAGTAAAATTCTGGCTTTCCAttt > 1:7968662/1‑76 (MQ=255) aGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGGCAGGAAAATTCTGTCTCGCCCttt > 1:5537568/1‑76 (MQ=255) aGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAACCCGTAAATTTCTTTCTGGCCCttt > 2:5372887/1‑76 (MQ=255) gACATCATGCTCTTTGCGGGGGTGTAAGAAGAAGGTTGGGCGGCGGGGAGCCAGTAAATTTCTGTCTCGCCATTTc < 1:4352976/76‑1 (MQ=255) aCATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGGGCCAGCAAAATTCTTTCTCTCCAttttt > 1:4653916/1‑74 (MQ=255) aCATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGGAAAATTCTTGTTCGCCActtta > 1:1442847/1‑74 (MQ=255) atcttGCGTTTTGCGGGGGTGTAAGAAGAAGAGTGGGCGGCGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCaaa < 1:5257680/76‑1 (MQ=255) atcatGCTCTTTACGGGGGTGTCAGAAGGAGAGTTGCCGGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCaaa < 1:1657236/76‑1 (MQ=255) atcatGCGCTTGACGGGGTTGGAAGAAGAAGGGTTGGCGGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCaaa < 1:8586159/76‑1 (MQ=255) atcatGCCCTTACCGGGGGGGTAAAAAAAGGTGTGGGCTGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCaaa < 2:759701/76‑1 (MQ=255) gcgcTTTGCGGGGGTGTAAGAAGAGGGGTGGGCGGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTg < 1:516449/76‑1 (MQ=255) tttACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGGGCCAGTAAATTTCTGGCTCGCCATTTCAAAAGTTGCCTa > 2:6457295/1‑76 (MQ=255) tcccGGGGGGTAAGAAAAAGGGTGGGCTGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTaca < 1:2227624/74‑1 (MQ=255) gcgggggTGTAAGAAGAGGAGTGGGCGGCGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTacac < 2:8733801/73‑1 (MQ=255) aCCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTacac > 2:8812115/1‑76 (MQ=255) cgggggTGAAAGAAGAGGTGTGGGCTGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACAcc < 2:4731086/74‑1 (MQ=255) gggggggTAAGAAGAAGGGTGGGCGGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACAccc < 1:5962387/75‑1 (MQ=255) cGGGGTGTCAGAAGAAGAGTTGGCTGGGGCGGGCCAGGAAATTTTTGTCTCCCCATTTCAAAAGTTGCCTACAccc > 2:7231166/1‑76 (MQ=255) cGGGGTGTCAGAAGAAGAGTTGGCTGGGGCGAGCCCGTTAAACTCTGTCCCCCCATTTCAAATATTGTCTTCAccc > 2:7750858/1‑76 (MQ=255) cGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCCTTTCAAAAGTTGCCCACAccc > 2:2519846/1‑76 (MQ=255) ggggggTAAGAAGAGGAGTGGGCGGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCct < 2:8453042/76‑1 (MQ=255) ggggTGTAAGAAGAGGGGGTGGCTGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCct < 2:613904/76‑1 (MQ=255) gggTGTCAGAAGAAGAGTTGGCTGCGGGGAGCCAGGAAAATTTTGTCTCGCCCTTTTAAAAAGTGGCCACAccccc > 1:842838/1‑74 (MQ=255) gggTGTAAGAAGAAGTGTGGGCTGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCctc < 1:4881609/76‑1 (MQ=255) gggTGTAAGAAGAAGAGTGGGCGGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCctc < 2:1241347/76‑1 (MQ=255) gttgtAAGAAGAAGGGTGGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCctct < 1:4042644/74‑1 (MQ=255) ggTGTAAGAAGAGGGGTGGGCGGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCctct < 1:7817269/76‑1 (MQ=255) ggTGTAAGAAGAAGAGTGGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCctct < 2:2864954/76‑1 (MQ=255) gtgtCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTg > 1:5512847/1‑76 (MQ=255) tgtCAGAAGAAGAGTTGGCTGCGGCGGGCCAGTAAATTTCTGTCTCGCCATTTTAAAAGTTGCCTACACCctcctc > 2:3518120/1‑73 (MQ=255) ggaagaagagggGTGGGCGGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCt < 1:1574042/73‑1 (MQ=255) agaagaagaGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCCTTTTAAAAGTTGCCTACACCCTCTGCTTCa > 2:2388372/1‑76 (MQ=255) agaagaagaGTGGGCGGCGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCa < 1:610741/76‑1 (MQ=255) gaagaagaGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAATTGCCTACACCCTCTGCCTcca > 2:4529734/1‑74 (MQ=255) gaagaagaGGGGGCGGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCaa < 1:1115793/76‑1 (MQ=255) gaaaaagtGTGGGCGGCGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCaa < 1:5817071/76‑1 (MQ=255) aagaagaGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAAc > 2:1151410/1‑76 (MQ=255) aagaagaGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCCCGCCATTTCAAAAGTTGCCTACAACCTCTGCTTCCAc > 2:2632077/1‑76 (MQ=255) agagTTGGCTGGGGGGAGCCAGTAAATTTCTGTCTCGCCCTTTCAAAAATTGCCTACACCCTCTGCTTCAACGccc > 2:3134077/1‑75 (MQ=255) ggtGGCGGGGGGGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGccacca < 1:3048217/74‑1 (MQ=255) ttGGCTGGGGGGAGCCAGGAAAATTTTGTCTCCCCCTTTTAAAAGTTGCCTACACCCTCTGCTTCTACGccacccg > 2:2050587/1‑74 (MQ=255) ttGGCTGCGGGGAGCCAGTAAATTTTTTTCTCGCCCTTTCAAAAGTTGACTTAACCCCCTGCTTCAAAGccaccac > 2:2187352/1‑75 (MQ=255) ggCTGGGGCGGGCCAGAAAATTTCTGTCTCGCCATTTCCAAAGTTGCCTACACCCTCTGCTTCCACGGCACcagca > 2:1457277/1‑76 (MQ=255) ggCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCCCCGCTTCAACGCCACcagca > 2:4369854/1‑76 (MQ=255) ggCTGCGGCGAGCCAGGAAATTTCTGTCTCGCCATTTCAAAAGATGCCTACACCCTCTGCTTCAACGCCCCcagca > 2:2410030/1‑76 (MQ=255) gccgcggggAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACcagcag < 2:4251138/73‑1 (MQ=255) gCTGCGGCGGGCCAGTAAATTTCTGTCTTCGCGCTTCGAAAGATTCCCACACCCCCTGCTTTAATCCCACccgcag > 2:7145331/1‑76 (MQ=255) gCTGCGGCGGGCCAGTAAATTTCTGTCTCGTCATTTCAAAAATTGCCTTCACCCTCCACCTCAAATCCACcagcag > 2:8326183/1‑76 (MQ=255) gCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCCTAACCCTCTGCTTCCACGACACcagcag > 2:8980023/1‑76 (MQ=255) cTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAgg > 2:8246117/1‑76 (MQ=255) cTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCAACAGCAgg > 2:5694164/1‑76 (MQ=255) gcggcgAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTg > 2:7008449/1‑76 (MQ=255) cggcgAGCCCGGAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGCGa > 2:5739592/1‑76 (MQ=255) cggcgAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCCGGTGa > 2:6546195/1‑76 (MQ=255) cgAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACaa > 2:1965/1‑76 (MQ=255) cgAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACaa > 1:8275868/1‑76 (MQ=255) cgAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACaa > 2:3542952/1‑76 (MQ=255) | TAATGCTTGAATCCGGAGTCCCTCTTACAGACATCATGCGCTTTACCGGGGTGTCAGAAGAAGAGTTGGCTGCGGCGAGCCAGTAAATTTCTGTCTCGCCATTTCAAAAGTTGCCTACACCCTCTGCTTCAACGCCACCAGCAGGTGACAA > REL606/3472625‑3472775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |