| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,774,914 | 0 | C | G | 42.0% | 280.7 / 218.9 | 219 | A20P (GCC→CCC) | yicJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (3/124); new base G (92/0); total (95/124) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 291 total aligned reads displayed.
CGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774839‑3774989 | cGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGtggctggc < 2:6702862/76‑1 (MQ=255) gCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggcgg < 2:7680506/76‑1 (MQ=255) cAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTgggggg > 1:229765/1‑75 (MQ=255) aGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggggggg > 2:8763038/1‑74 (MQ=255) aGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggggggg > 2:6004337/1‑74 (MQ=255) ggAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGt < 1:6396575/76‑1 (MQ=255) gCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTccc < 2:1703471/76‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCa < 1:7582110/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTccccc > 2:1614617/1‑74 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 2:886647/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 1:8711817/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 1:6813130/76‑1 (MQ=255) aaaaaTATCTGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTcccctc > 1:202656/1‑73 (MQ=255) aaaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGcc < 1:1865541/76‑1 (MQ=255) atatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAt < 2:2984726/76‑1 (MQ=255) atatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAt < 2:7755383/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑a < 2:6549556/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑a < 2:4038602/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑a < 2:3918214/76‑1 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑a < 1:5397028/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑aa < 1:8377741/76‑1 (MQ=255) tCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AAc < 1:1931688/76‑1 (MQ=255) cGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AAcc < 2:6471867/76‑1 (MQ=255) gTATAAAAGAACATCATATATTACATTTCGTTATCTAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCaa < 2:4085353/76‑1 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCTGCCCA‑AACCaaa > 2:3656967/1‑75 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTTCCAT‑AAcccaa > 1:5066598/1‑73 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCATGCCCT‑CAcccaa > 2:3323646/1‑73 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGCGGCTCCCCTCGCCT‑AACCaaa > 1:411124/1‑75 (MQ=255) ataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCACGCCCT‑AACCaaat > 2:4152817/1‑74 (MQ=255) ataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCTAAACCAAt > 1:2392306/1‑76 (MQ=255) taAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCCT‑CACCAAatt > 2:7273397/1‑76 (MQ=255) aaaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGGGGCTCCCCAGCCCT‑AACCAAattt > 2:1863867/1‑76 (MQ=255) aaaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAAtttt < 2:3788316/76‑1 (MQ=255) aaaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTtc < 2:7018956/76‑1 (MQ=255) aaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGTCTCCCACACCTC‑CACCAAtttttt > 2:1984186/1‑74 (MQ=255) aaGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCAT‑AACAAAATTtct > 1:7844535/1‑76 (MQ=255) aaCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCAT‑AACCACTTTTTT‑Ctt > 2:2032389/1‑76 (MQ=255) aaCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑Ctt < 2:2694658/76‑1 (MQ=255) aaCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑Ctt < 2:1208815/76‑1 (MQ=255) aCATCATATATAACATTACTTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑Cttt < 1:3159975/76‑1 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑Cttt < 2:5088434/76‑1 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑Cttt < 2:6228872/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGTTTCCCATGCCAT‑AACCAATATTCT‑ttttt > 1:1721961/1‑75 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTa < 2:5710875/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTa < 2:4291762/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTa < 1:3975731/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTa < 1:8441878/76‑1 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCTGCCCT‑CATCACtttttt‑ttttta > 2:4871564/1‑74 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCAC‑AACCAAATTTTT‑Ctttta > 2:8045197/1‑74 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTaa < 1:7465314/76‑1 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTaa < 2:7533335/76‑1 (MQ=255) tcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCTGCCCT‑AACCAAATtttt‑tttttat > 2:7847160/1‑73 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AACCCAATTTTT‑CTTTTAcc > 1:7910529/1‑75 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGCGGCCCCCCCGCCAT‑TATCAATTTTCT‑TTTTAAcc > 1:887883/1‑75 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACg < 1:36890/76‑1 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACg < 1:12636/76‑1 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACg < 2:5568226/76‑1 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACg < 1:6589564/76‑1 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCTGCCCT‑AACCAAATTTTT‑TTTTTActt > 2:6815933/1‑74 (MQ=255) atatatAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACgg < 1:4746628/76‑1 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AACCAAATTTCT‑CTTTTACGGac > 2:2476820/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCTCCCT‑AACCAATTTTCT‑TTTTTACAGac > 2:4776977/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGac < 1:5229488/76‑1 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGac < 1:2775139/76‑1 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGac < 2:8348383/76‑1 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGac < 1:7286182/76‑1 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTTCCCT‑AACCAAATTTTT‑CTTTTACGGaca > 1:3924913/1‑76 (MQ=255) ataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCCCCCCTGCCCT‑AACCAATTTTTT‑CTTTTATTGacaa > 2:5494604/1‑76 (MQ=255) ataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGacaa < 1:6271411/76‑1 (MQ=255) ataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGacaa < 2:22219/76‑1 (MQ=255) aaCATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTCCCCT‑AACCCATTTTCT‑TTTTTACGGacaaaa > 1:5580929/1‑74 (MQ=255) aCATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAacac < 1:6004337/76‑1 (MQ=255) cATTACGTTATCGAAAATAATGTGGCTGGGGGGGCCTCCCCTACCAA‑AAACAAATTTTT‑CTTTTACGGCCAACACt > 1:3303694/1‑76 (MQ=255) cATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACt < 1:717594/76‑1 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCT‑AACCAAATTTTT‑CTTTTAAGGACAAAAATTc > 1:700743/1‑76 (MQ=255) aCGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCCT‑AACCAATTTTCT‑CTTTCACGGACAACACCTCAc > 2:6628394/1‑76 (MQ=255) ttATCGAAAATAATGTGGCTGGGGGGGGGGCCCCTGCGAT‑AAACAATTTTTTCCTTTAAC‑GTCCACACATCActct > 2:5215861/1‑76 (MQ=255) aTCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTc < 1:1626949/76‑1 (MQ=255) cGAAAATAATGTGGCTGGGGGGGCCTCCCCTTCCCT‑CACCAACTTTTT‑TTTTTTTGCACAACACTCTACACTTcct > 2:2305717/1‑74 (MQ=255) gAAAATAATGTGGCTGGGGGGGGCTCCCATGCCAT‑AAACAATTTTCT‑TTTTAACGGACAACAACTCACTCTTCCTc > 2:5407131/1‑76 (MQ=255) gAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATc < 2:3536729/76‑1 (MQ=255) aaaaTAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCt < 1:6745701/76‑1 (MQ=255) aaaaTAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCt < 1:3353006/76‑1 (MQ=255) ataatGTGGCTGGGGGGGGCTCCCCTGCCCT‑AACCAAATTTTT‑CTTTTACGGACAACACTTCACTCTTTCTCTGCt > 1:1068063/1‑76 (MQ=255) ataatGTGGCTGGGGGGGGCTCCCATGCCAT‑AAACAATTTTTT‑CTTTTACAGACAACAACTCACCCTTCAtcttct > 2:711313/1‑76 (MQ=255) ataatGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCt < 1:8141499/76‑1 (MQ=255) taatGTGGCTGGGGGGGGCTCCCCTGCCCT‑AAACAATTTTTT‑TTTTTACAGACAACAACTCACTCTTCATCTGCtt > 2:4521124/1‑76 (MQ=255) aatGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCttt < 2:6334475/76‑1 (MQ=255) atGTGGCTGGCGGGGGCTCCCATGCCAT‑AACCAATTTTCT‑CTTTTACGGACAACACTTCACTCTTCATCTGCtttt > 2:3118061/1‑76 (MQ=255) tggctggcGGCGTCTCCCATGCCAT‑AAACAATTTTCT‑CTTTAACGGAAAACACTTCACTTTTCATCTGCTTTTCCt > 2:1047608/1‑76 (MQ=255) ggctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCCTCTGCTTTTCCtt > 1:3525193/1‑76 (MQ=255) ggctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCtt < 1:5925289/76‑1 (MQ=255) ggctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCtt > 1:4329611/1‑76 (MQ=255) gctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTg > 1:381623/1‑76 (MQ=255) ctggcGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGa < 2:8445802/76‑1 (MQ=255) ggcggcGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATg < 2:1624900/76‑1 (MQ=255) ggcggcGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATg > 1:496951/1‑76 (MQ=255) ggcggcGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATg > 1:4835696/1‑76 (MQ=255) ggcggcGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATg > 1:4649254/1‑76 (MQ=255) cggcgTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCt > 1:8069394/1‑76 (MQ=255) | CGGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCAT‑AACCAATTTTCT‑CTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774839‑3774989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |