| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,392,556 | 0 | C | T | 36.8% | 250.1 / 121.8 | 191 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (79/41); new base T (0/70); total (79/112) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-23 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 282 total aligned reads displayed.
AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 | aGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGGTTTTc > 2:2801409/1‑76 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 2:6682744/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 1:3124256/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 2:8501944/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 2:4276806/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa > 2:568565/1‑76 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGGAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAg > 1:2618737/1‑76 (MQ=255) aCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGc < 2:2865018/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGGGGTTTTTTAGCCa > 1:6224988/1‑76 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa > 2:2121408/1‑76 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa > 2:4448100/1‑76 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa < 2:2223167/76‑1 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGGGATTTTCTAGcccg > 2:7273994/1‑74 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg < 1:4904639/76‑1 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg < 1:4111473/76‑1 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAga > 2:865465/1‑75 (MQ=255) aCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTAt > 1:4205422/1‑76 (MQ=255) ccggTCTGATGGCGCGCTCTGGGAAGCGGTAGCGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTAtt < 2:3799978/73‑1 (MQ=255) ccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGCCAGTAtt > 1:4795405/1‑76 (MQ=255) ccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTAtt < 2:82529/76‑1 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTg > 1:6651293/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGGATTg > 1:2393649/1‑76 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTTTTGCCAGTATTgg > 1:7137545/1‑76 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGAACCGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTgg < 2:1873410/76‑1 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGCCAGGATTGGt > 1:5140570/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGt > 2:1183499/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGAAAGGGATTTTCTACCCAGCATTGGt > 2:6433982/1‑76 (MQ=255) tCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTc > 2:8032249/1‑76 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTTTAGCCAGGATTGGGca > 1:5123538/1‑76 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTTGTca > 2:3269245/1‑76 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTTGTca > 1:5427239/1‑76 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGAGTTTCTAGCCAGTATTGGTca > 2:3682571/1‑76 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTcag < 2:1475639/76‑1 (MQ=255) tGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTcag > 2:5731579/1‑76 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTTGCCAGTATTTGTtagc > 1:7196497/1‑76 (MQ=255) gATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTcagc > 1:6131351/1‑76 (MQ=255) aTGGCGCGCTCTGCGAAGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTcagca < 2:5154485/76‑1 (MQ=255) aTGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTcagca > 1:202715/1‑76 (MQ=255) gccgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCGGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGt < 1:6764035/74‑1 (MQ=255) cgcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTa > 1:5539189/1‑76 (MQ=255) gcgcTCGGCGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAg < 2:6552933/76‑1 (MQ=255) gcgcTCGGCGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAg < 1:976775/76‑1 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAgg < 1:825913/76‑1 (MQ=255) gcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGa > 1:2794854/1‑76 (MQ=255) gcTCGGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGa < 2:5576093/76‑1 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCCGTATTGGTCAGCAGTTAGGaa > 2:6509357/1‑76 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGaa > 1:3954405/1‑76 (MQ=255) ctatGCGAAGGGGTAACGCTTGAAGGTCCTGTCGGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:2091269/73‑1 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGGACg > 1:5248953/1‑76 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACg > 2:7112176/1‑76 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACg < 2:6136789/76‑1 (MQ=255) tGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACgg < 2:1506038/76‑1 (MQ=255) ggCGAGGCGGTAACGCGTGAGGGTGCTGGCTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACgg < 1:7948202/75‑1 (MQ=255) ggCGAAGCGGTAACGCGTGAAGGTGCGGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACgg < 1:2215003/75‑1 (MQ=255) ggAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt < 1:5666261/75‑1 (MQ=255) gAAGCGGCAACGTGTGAAGGTGTTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTg < 1:8683391/76‑1 (MQ=255) aaGCGGCACGGCGAGAAGGTGCTGGGGGTGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 2:4557798/76‑1 (MQ=255) gTAACGCGTGAAGGTGCTGGCTGGGATAGCTATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATccc < 2:2591556/76‑1 (MQ=255) tAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCt < 1:1738743/76‑1 (MQ=255) tAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGGATTCCt > 1:1000035/1‑76 (MQ=255) aCGCGTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTg < 2:3888166/76‑1 (MQ=255) gggTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGTTGAATCCCTTGaa < 2:8066514/74‑1 (MQ=255) gggTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa < 2:931146/74‑1 (MQ=255) ggTGAGGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAAc < 2:459848/75‑1 (MQ=255) ggTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAAc < 1:428775/75‑1 (MQ=255) gggAAGTTGCGGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACt < 1:1534717/74‑1 (MQ=255) gTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGGACGGCTGAATCCCTTGAACt > 1:2981606/1‑76 (MQ=255) tGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTg > 1:8488425/1‑76 (MQ=255) gAAGGGGCGGTCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgg < 2:585581/76‑1 (MQ=255) gTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCCGAAAACCCTGGAATgggggg > 2:3391223/1‑74 (MQ=255) gctggctgGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCCTTAACTGGgggggc > 1:7430802/1‑76 (MQ=255) ctggctggGATAGCGTTTTTTTAGCCAGTATTGGTCGGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCt < 2:4768037/76‑1 (MQ=255) tggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTg < 2:4919277/76‑1 (MQ=255) gggctggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTg < 2:3034596/75‑1 (MQ=255) gggtggGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:6771968/73‑1 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGGTgg > 2:6957362/1‑76 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg > 2:3998044/1‑76 (MQ=255) gctggGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGc < 1:914737/76‑1 (MQ=255) gcggggATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGc < 2:359634/73‑1 (MQ=255) gtggGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAGCGGCTGAATCCCTTGAACTGGGGCGGCTGGCg < 1:4696731/75‑1 (MQ=255) tggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCgg > 1:112944/1‑75 (MQ=255) gATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGCAtg > 1:771548/1‑76 (MQ=255) atggCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgt < 1:3862393/73‑1 (MQ=255) aTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGGAtgt > 1:5810717/1‑76 (MQ=255) aTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTCGGGACtgt > 1:5063152/1‑76 (MQ=255) tAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAAtgtg < 1:8757901/76‑1 (MQ=255) cGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGGATGTGaaa > 1:1323678/1‑75 (MQ=255) gtttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc < 2:5389721/74‑1 (MQ=255) gtttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc < 2:5952641/74‑1 (MQ=255) gATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATc > 2:7475182/1‑76 (MQ=255) ttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCg < 1:3493245/76‑1 (MQ=255) tttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGGGGAACCGc > 1:8158489/1‑76 (MQ=255) ttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGGAAGTGAAACCggc > 1:4553762/1‑74 (MQ=255) tCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGGATGTGGATACGccc > 1:7537372/1‑75 (MQ=255) tCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGGATGTGAATCCGCCt > 1:5922568/1‑76 (MQ=255) tCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCt > 1:1208201/1‑76 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGGGAAAGTGGATACGCCTg > 1:3719064/1‑76 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGGATGTGAATCCGCCTg > 1:7014744/1‑76 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATTTGAATCCCCCTg > 1:5749945/1‑76 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGcccg > 1:2841357/1‑74 (MQ=255) | AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |