| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 931,314 | 0 | T | C | 39.4% | 112.0 / 50.0 | 104 | E84G (GAG→GGG) | ybjE | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (37/26); new base C (1/40); total (38/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.68e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 222 total aligned reads displayed.
AGCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCA > REL606/931239‑931389 | aGCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCt > 2:2056238/1‑76 (MQ=255) ggACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTcc < 1:724259/75‑1 (MQ=255) cGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTcc > 2:2827373/1‑76 (MQ=255) cGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTcc < 2:6738814/76‑1 (MQ=255) gACTCCAGCGCCATCGCAATATGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCCCGCTccc > 2:2885939/1‑75 (MQ=255) gACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGctccctccc > 2:1342538/1‑75 (MQ=255) gACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCa < 2:5739497/76‑1 (MQ=255) gACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCa < 1:7438386/76‑1 (MQ=255) aCTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCTCTCCAg > 2:5394346/1‑76 (MQ=255) aCTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCCCCCCAg > 2:4555939/1‑76 (MQ=255) cTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCAGc > 2:3058514/1‑76 (MQ=255) tCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCAGcc > 1:5931451/1‑76 (MQ=255) ccAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCCCGCTCCAGccc > 1:3387440/1‑75 (MQ=255) cAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCCCGCTccacccac > 2:2479509/1‑76 (MQ=255) aGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCCCCCGCccca > 1:5813650/1‑76 (MQ=255) aGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCCCGCTCCCGCcaca > 2:3152843/1‑76 (MQ=255) cgcCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATc < 1:6391867/76‑1 (MQ=255) gcCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCAGCCACCTCa > 1:6082156/1‑76 (MQ=255) ccATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAg < 2:1871630/76‑1 (MQ=255) ccATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCGCGCTCCAGCCACATCAg > 2:3875640/1‑76 (MQ=255) ccATCACAATCCGGGACGGGAGTTTTTCTTGCTGTTGGTGGTCGCGCCACGGCAGGCCCCCCCCCAGCCACATCAg < 2:5314113/76‑1 (MQ=255) cATCGCAATACGCGACGGGATTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAgg < 1:5642929/76‑1 (MQ=255) cATCCCAATACAGCACGGGAGTTTTTCTTGCTGATGGTGGTTGCCCCACGGCAGACCCCCCTCCAGCCACATCAgg < 2:2007533/76‑1 (MQ=255) cGCAATACGCGAGGGGATTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGggcg < 2:3348750/76‑1 (MQ=255) cAATCCGCGCCGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGggcggc < 2:2595233/76‑1 (MQ=255) cAATACGCGACGGGATTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGggcggc < 2:6501234/76‑1 (MQ=255) cAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCCCGCTCCCCCCCCATCAAgggggg > 1:3058393/1‑75 (MQ=255) aaTACGCGGGGGGATTTTTTCTTGCTGGTGGTGGTTGCCCCGCGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCa < 2:3984251/76‑1 (MQ=255) aaTACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCAGCCACATCCGGGCGGCa > 2:5545709/1‑76 (MQ=255) aaTACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCCCGCTCCAGCCACATCCAGGCGGCa > 2:8283871/1‑76 (MQ=255) aaTACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCa < 1:7782997/76‑1 (MQ=255) aTACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCaa < 1:8578545/76‑1 (MQ=255) tACGGGAGGGGTTTTTTTTTTGCGGATGGGGGTTGCGCCACGGCAGGCCCCGCTCCAGCCACATCAGGGCGGCAa‑t < 2:5318944/76‑1 (MQ=255) tACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAa‑t < 1:404349/76‑1 (MQ=255) aCGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAa‑ta < 1:2340277/76‑1 (MQ=255) ggACGGGAGTTTTTCTTGCTGATGGGGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTa < 1:1347049/75‑1 (MQ=255) gcGGGATTTTTTCTTGCTGATGGGGGTTGCCCCCCGGCGGGCCTCCCCCCAGCCACATCAGGGCGGCAA‑TATTaca < 2:6126013/75‑1 (MQ=255) aggggAGTTTTTCTTGCTGATGGTGGTTGCCCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTaca < 2:600313/74‑1 (MQ=255) ggAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCCGCCCCATCAGGGCGGGAA‑TATTAAACAg > 1:7085544/1‑76 (MQ=255) ggAGTTTTTCTTGCTGAGGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAg < 1:4288563/76‑1 (MQ=255) gAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCCCGCTCCCGCCACATCAGGGCGGCAC‑AATTAcacaag > 2:1614636/1‑74 (MQ=255) gAGTTTTTCTTGCGGATGGTGGTTGCGCCACGGCAGCCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGt < 1:2445911/76‑1 (MQ=255) gAGTTTTTCTGGCTGATGGTGGTGGCGCCAGGGCAGCCCCCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGt < 2:865705/76‑1 (MQ=255) aGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCCGCCACATCAGGGCGGCAA‑TATTACACAGTa > 1:512413/1‑76 (MQ=255) aGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCCA‑TATTACACAGTa > 1:4201887/1‑76 (MQ=255) tttttCTTGCTGATGGTGTTTGCCCCACGGCAGCCCTCCCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTaaa < 1:5063885/76‑1 (MQ=255) ttttCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTaaaa < 2:5896808/76‑1 (MQ=255) ttCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCCCATCAGGGCGGCAA‑TATTACACAGTAAAATa > 1:3793207/1‑76 (MQ=255) tCTTGTTGATGGTGGTTCCTCCACGGCAGCCCCCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATaa < 1:8201584/76‑1 (MQ=255) tCTTGCTGATGGTGGTTGCGCCACGGCAGGGCCCGCTCCAGCCACCTCAGGGCGGCAA‑TAATACACAGTAAAATaa > 2:4883536/1‑76 (MQ=255) tCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGGAA‑TATTACACAGTAaaaaaa > 1:5346654/1‑76 (MQ=255) cTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAATAaaaaaaa > 1:2795390/1‑75 (MQ=255) ttGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCACGCACATTACGGCGGCAA‑TATTACACCGTAAAAAAACg > 2:6200435/1‑76 (MQ=255) ttGCTGAGGGTGTTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACg < 2:8838014/76‑1 (MQ=255) gCTGATGGTGGTTGCGCCCCGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGt < 2:146966/76‑1 (MQ=255) gCTGATGGTGGTTGCGCCCCGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGt < 1:6911368/76‑1 (MQ=255) gCTGATGGTGGTTGCGCCACGGCAGGCCTTGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACggc > 1:4451549/1‑75 (MQ=255) gCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGt > 1:3493137/1‑76 (MQ=255) gCTGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGt < 2:2654364/76‑1 (MQ=255) cTGATGGTGGTTGCGCCACGGCAGGCCCCCCTCCCGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACCGTa > 2:4360164/1‑76 (MQ=255) tGATGGTGGTTGCGCCACGGCAGGGCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTaa > 2:5764228/1‑76 (MQ=255) tGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCCGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTaa > 2:6886793/1‑76 (MQ=255) tGATGGTGGTTGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTaa < 1:1612086/76‑1 (MQ=255) tGATGGTGGTTGCGCACCGGCAGGCCCCCCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTaa < 2:8507831/76‑1 (MQ=255) gATGGTGGTTGCGCCACGGCAGGGCCCCCCCCAGCCACAACAGGGCGGCAA‑TATTACACAGTAAAAAAAAGGTaaa > 1:8884307/1‑75 (MQ=255) gATGGTGGTGCCGCCACGGCAGGCCCCCCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAAt < 1:6510714/76‑1 (MQ=255) aTGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCCAGGGGGCAA‑TATTACACAGTAAAAAAACGGGAATa > 1:151848/1‑76 (MQ=255) aTGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATa > 1:5664899/1‑76 (MQ=255) ggtggtTGCCCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACt < 2:7429867/76‑1 (MQ=255) ggtggtGGCGCCACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACt < 2:8372768/76‑1 (MQ=255) gtggtTGCGCCACGGCAGGCCTCCCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTg < 1:7132590/76‑1 (MQ=255) gggtGGCCCCAGGGGAGGCCTCCCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGa < 1:5156/75‑1 (MQ=255) ggtTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGAc > 2:3771556/1‑76 (MQ=255) gtTGCGCCACGGCAGGCCTCGCACCTTCCACAGCAGTTCGGCAAGTATT‑TTTAGTTACATAACGGCAATACTgtac > 2:2733651/1‑73 (MQ=255) tGCGCCACGGCAGGGCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGc > 2:3520771/1‑76 (MQ=255) tGCGCCACGGCAGGCCTCGCTCCAGCCACCTCAGGGCGGCAA‑TATTACACAGTAAAAAAACGGTAATACTGACAGc > 2:7154765/1‑76 (MQ=255) cgcCAGGGCAGCCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAg < 1:1394542/76‑1 (MQ=255) cgcCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAg < 2:9003736/76‑1 (MQ=255) ccAGGGCGGCCCCCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGaa < 1:1245167/76‑1 (MQ=255) ccACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑AATTACACAGTAAAATAACGGGAATACTGACGGCAGaa > 1:4580701/1‑76 (MQ=255) cgcGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGaat < 1:6575696/74‑1 (MQ=255) cACGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGaat < 2:8753517/76‑1 (MQ=255) cggcggCCCTCCCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGaataa < 1:7983430/76‑1 (MQ=255) cGGCAGGCCTCGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGaataa < 2:7241003/76‑1 (MQ=255) cGGCAGGCCACCCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGaataa < 1:1029592/76‑1 (MQ=255) cGGCAGCCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGaataa < 2:6793687/76‑1 (MQ=255) ggCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGaataat > 2:6633135/1‑76 (MQ=255) gCAGGTCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATg > 2:7829104/1‑76 (MQ=255) aGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCa > 2:1199576/1‑76 (MQ=255) gCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGa > 2:566917/1‑76 (MQ=255) cccGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAAt < 2:3622588/74‑1 (MQ=255) ccGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATc < 1:2583278/75‑1 (MQ=255) ccGCCCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATc < 2:4054010/75‑1 (MQ=255) cGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCg > 1:8983036/1‑76 (MQ=255) cGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCg > 1:6180436/1‑76 (MQ=255) gCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGc > 1:7303640/1‑76 (MQ=255) cTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGcc > 2:731024/1‑76 (MQ=255) cTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGcc > 2:7954618/1‑76 (MQ=255) cTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGcc > 1:8901955/1‑76 (MQ=255) cTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGcc > 2:5083555/1‑76 (MQ=255) | AGCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAA‑TATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCA > REL606/931239‑931389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |