| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,485,450 | 0 | A | G | 35.7% | 421.3 / 156.9 | 241 | G193G (GGA→GGG) | ydcV | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (6/149); new base G (85/1); total (91/150) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 288 total aligned reads displayed.
GGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTA > REL606/1485375‑1485525 | aTCTGGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑Caggagg < 2:3740677/76‑1 (MQ=255) cTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGGAGTGTTGCCGAATCTCCGTTCGGCGTTACTGG‑Caggggggg > 2:2403315/1‑74 (MQ=255) ttGGGGCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAAt < 1:5825405/76‑1 (MQ=255) ttGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑Cagggggggg > 2:6947927/1‑73 (MQ=255) ttGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑Cagggggggg > 2:5810261/1‑73 (MQ=255) ttGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAAt < 2:1307905/76‑1 (MQ=255) ttGGGGCCAATGGCTGGCAAACCTTACGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAAt < 1:3194880/76‑1 (MQ=255) tGGGGCCAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATg < 2:3524473/76‑1 (MQ=255) gggCCAATGGCTGGGAAACCTTCCGCTACGTAGGGTTGCCGAATCTCACTTCGGCGTTACTGG‑CAGGGGGGAtagt > 2:8661135/1‑73 (MQ=255) ggCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTg < 2:5272021/76‑1 (MQ=255) ccAATGGCTGGCAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGc < 1:2851988/76‑1 (MQ=255) cAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCCGGGGGGAATGCgggg > 1:1970355/1‑75 (MQ=255) agggctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑Gtt < 2:2794285/74‑1 (MQ=255) gctggcAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGc < 1:334546/76‑1 (MQ=255) gctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGc < 1:3763908/76‑1 (MQ=255) ctggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGGTGGC‑GGTTGcc > 1:1481102/1‑76 (MQ=255) tggcAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCt < 1:7555669/76‑1 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGACGCTGGC‑GGTTGTCt > 2:1893809/1‑76 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGAAGCTGGG‑GGTTTCCt > 1:7775476/1‑76 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGAATGCTGGC‑GTGTGTCt > 2:4164386/1‑76 (MQ=255) tggcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGAAAGCTGGCGGTTTGct > 1:6578901/1‑75 (MQ=255) gccAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCtt < 2:4587009/74‑1 (MQ=255) gcAACCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTg < 1:3055552/76‑1 (MQ=255) gcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGGAGCTGGC‑GTTTTccctt > 1:8003068/1‑73 (MQ=255) gcAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTg < 2:6555262/76‑1 (MQ=255) cAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGt < 2:2318994/76‑1 (MQ=255) aaaCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGAAGCTGGG‑GGTTGGCTTGTc > 1:3726946/1‑76 (MQ=255) aaaCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTc < 1:8102142/76‑1 (MQ=255) aaCCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGAATGCTGGC‑GGTTGCCCTGTCg > 2:1043462/1‑76 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGAAGCTGGG‑GGTTGCCTTTTCGtt > 2:5159372/1‑76 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGtt < 1:2762833/76‑1 (MQ=255) ccTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGtt < 1:4023171/76‑1 (MQ=255) cTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTc < 2:3093837/76‑1 (MQ=255) cTTCCGCTACGTAGTGTGGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTc < 2:758341/76‑1 (MQ=255) ttCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCg < 2:4390573/76‑1 (MQ=255) ttCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCg < 1:6264777/76‑1 (MQ=255) tcGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GGTTGCCTTGTCGTTCGAt < 2:2217924/75‑1 (MQ=255) gCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGGAGCTGGC‑GTTTTCCTTGTCGTTCGCTGa > 2:614430/1‑76 (MQ=255) gCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGa < 1:70520/76‑1 (MQ=255) gCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGa < 1:2308091/76‑1 (MQ=255) cTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGaa < 1:76370/76‑1 (MQ=255) tACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGC‑GGTTGCCTTTTCGTTTGATGaaa > 1:548405/1‑76 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGAAGCTGGC‑GTTTGCCTTTTCGTTTGATGgaaa > 1:3819411/1‑75 (MQ=255) aCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGAAGCTGGC‑GTTTGCCTTGTCGTTCGGTGAAat > 2:1970729/1‑76 (MQ=255) cGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGATGGC‑GGTTTCCTTTTCGTTTGATGAAatc > 2:7947286/1‑76 (MQ=255) cGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGGATGCTGGC‑GTTTGCCCTTTCGTTCGATGAAata > 1:4203900/1‑75 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAataa > 1:8971182/1‑74 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGAGGCTGGC‑GGTTGCCCTGTCGTTTGATGAAatca > 1:7193035/1‑76 (MQ=255) gTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGAATGCTGGC‑GGTTGCCTTTTTGTTTGTTGAAatca > 2:8089534/1‑76 (MQ=255) tAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGGTGGC‑GGTTGCCTTTTCGTTCGGTGCAaacat > 1:2552010/1‑76 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGAAGCTGGG‑GGTTGGCTTGTCGTTCGATGAAattatc > 2:5211049/1‑76 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGGATGGTGGC‑GGTTGGCTTGTCGTTCGTTGGAataatc > 1:5228735/1‑76 (MQ=255) aGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAatcatc < 1:7383500/76‑1 (MQ=255) gtgtTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGC‑GTTTGGCTTGTCGTTTGATGGAATTATCg > 1:8354829/1‑76 (MQ=255) gtgtTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCg < 1:5276128/76‑1 (MQ=255) gtTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGG‑GTTTGCCTTGTCGTTCGATGAAATAATCGtt > 2:1726124/1‑76 (MQ=255) ttGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGG‑GTTTTCCCTTTTGTTCGATGAAATCATCGTTa > 2:2593232/1‑76 (MQ=255) ttGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGC‑GGTTGCCCTGTTGTTCTGTGGAATAATCGTTa > 2:7708785/1‑76 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGAAAGCTGGG‑GGTTGCCCTGTCGTTCGATGAAAACAATGTTaa > 1:4961062/1‑75 (MQ=255) tGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTAc < 1:1360768/76‑1 (MQ=255) gCCGAGTCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACg < 2:7410217/76‑1 (MQ=255) gCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGC‑GTTTGCCTTGTCGTTCGTTGAAATCATCGTTAcc > 2:1518688/1‑75 (MQ=255) gCCGAATCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGC‑GGTTGCCTTGTCGTTTGATGAAATCATCGTTACg > 2:7784633/1‑76 (MQ=255) cGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGAc < 2:2714328/76‑1 (MQ=255) gAATCTCAGTTCGGCGTTACTGG‑CAGGGGGAATGCTGGC‑GGTTTCCTTGTCGTTTGATGAAATCATCGTTACGAcc > 1:7343608/1‑76 (MQ=255) gAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGAcc < 1:5869349/76‑1 (MQ=255) aTCTCAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGG‑GTTTGTCTTGTCGTTCGATGAAATCATCGTTACGACCtt > 2:3306318/1‑76 (MQ=255) aTCTCAGTTCGGCGTTACTGG‑CAGGGGGGAGGCTGGC‑GGTTGCCTTGTCGTTCGGTGAAATCATCGTTACGACCtt > 1:1250970/1‑76 (MQ=255) aTCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCtt < 2:8745858/76‑1 (MQ=255) aTCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCtt < 2:5957923/76‑1 (MQ=255) aTCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCtt < 1:8751380/76‑1 (MQ=255) tctcAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCttt < 2:3973399/76‑1 (MQ=255) tctcAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCttt < 1:4395547/76‑1 (MQ=255) tctcAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCttt < 2:7862952/76‑1 (MQ=255) tctcAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCttt < 2:5030150/76‑1 (MQ=255) tctcAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCttt < 1:696433/76‑1 (MQ=255) ctcAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGG‑GTTTTCCCTGTCGTTCGATGAAAACATCGTTACGACCcttt > 1:5591739/1‑75 (MQ=255) ctcAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTTGATGAAATCATCGTTACGACCTTTa < 2:5589160/76‑1 (MQ=255) tcAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGG‑GTTTGCCTTTTCGTTTGATGAAATTATCGTTACGACCttttc > 1:4522936/1‑74 (MQ=255) tcAGTTCGGCGTTACTGG‑CAGGGGGGATGCTGGC‑GGTTGCCTTGTCGTTTGATGAAATCATCGTTACGACCctttc > 2:53324/1‑74 (MQ=255) tcAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTAc < 2:4774889/76‑1 (MQ=255) aGTTCGGCGTTACTGG‑CAGGGGGGATGGTGGC‑GTTTGGCTTTTCGTTTGTTTGAATAATCGCTAAGTCCCTTACgg > 2:7099185/1‑76 (MQ=255) tCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAg < 1:2890280/76‑1 (MQ=255) tCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAg < 2:4055384/76‑1 (MQ=255) cGTTACTGG‑CAGGGGGGATGCTGGG‑GGTTGTCTTGTCGTTCGATGAAAACATCGTTACTTACCTTACGGCAggggg > 2:6490583/1‑73 (MQ=255) cGTTACTGG‑CAGGGGGAATGGTGGC‑GTTTGCCTTTTCGTTCGATGAAAACATCGTTAAGACCTTTACGGCAggggg > 2:8364/1‑73 (MQ=255) cGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCa < 1:1632373/76‑1 (MQ=255) cGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCa < 2:2965723/76‑1 (MQ=255) gTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCAt < 2:6752494/76‑1 (MQ=255) gTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCAt < 2:2918947/76‑1 (MQ=255) ttACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATg < 1:296545/76‑1 (MQ=255) aCTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaa < 2:8548565/76‑1 (MQ=255) aCTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaa < 1:1099619/76‑1 (MQ=255) tGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacg < 2:2456356/76‑1 (MQ=255) tGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGGGTgaacg > 2:2186874/1‑76 (MQ=255) g‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaa < 2:7333106/76‑1 (MQ=255) g‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaa < 1:6702754/76‑1 (MQ=255) aggaggAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTATGGCAGGTCATGaacgaacg < 1:6542353/76‑1 (MQ=255) aggaggAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGGAGGaaggaacg > 2:8858321/1‑76 (MQ=255) aggaATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGGAGGGACGAACGGTa > 2:4517955/1‑76 (MQ=255) | GGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGG‑CAGGAGGAATGCTGGC‑GTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTA > REL606/1485375‑1485525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |