| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 474,743 | T→A | D188V (GAT→GTT) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 474,743 | 0 | T | A | 99.2% | 1033.2 / ‑1.3 | 250 | D188V (GAT→GTT) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base A (159/89); total (160/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
Only 100 of 270 total aligned reads displayed.
CGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGGCAGCAACACCAGCG > REL606/474670‑474815 | cGGCCCCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACg < 2:4655953/76‑1 (MQ=255)cGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACg < 1:7213227/76‑1 (MQ=255) gCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGAc > 1:10591/1‑76 (MQ=255) gCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGAc > 2:8429134/1‑76 (MQ=255) cACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACt < 2:4979727/76‑1 (MQ=255) cACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACt > 1:2706412/1‑76 (MQ=255) cccAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTg < 2:4813554/75‑1 (MQ=255) aCCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTg < 2:1114570/76‑1 (MQ=255) cAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCa < 1:198669/76‑1 (MQ=255) aGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCaa > 1:5497707/1‑76 (MQ=255) aGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCaa > 1:7861447/1‑76 (MQ=255) aGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCaa > 1:700790/1‑76 (MQ=255) aCGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAaga < 1:5948815/76‑1 (MQ=255) aCGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAaga > 2:5442832/1‑76 (MQ=255) cGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAagag < 1:3800035/76‑1 (MQ=255) cGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAagag < 1:4261393/76‑1 (MQ=255) cGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTgg > 2:394777/1‑76 (MQ=255) cGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTgg < 1:6959707/76‑1 (MQ=255) cGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTgg > 1:2521017/1‑76 (MQ=255) cGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTgg > 2:1253040/1‑76 (MQ=255) ccTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCaa > 1:3116090/1‑76 (MQ=255) ccTACCACCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCaa < 1:8669927/76‑1 (MQ=255) cTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCaaa < 2:3710905/76‑1 (MQ=255) cTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCaaa < 2:8771621/76‑1 (MQ=255) cTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCaaa > 2:5366965/1‑76 (MQ=255) tACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAg < 2:1948382/76‑1 (MQ=255) aCCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGc > 1:3718585/1‑76 (MQ=255) ccAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGcc > 2:5539634/1‑76 (MQ=255) cAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGccc > 2:6584602/1‑76 (MQ=255) cAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGccc < 2:8296971/76‑1 (MQ=255) cAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGccc > 1:2623089/1‑76 (MQ=255) aGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCca > 2:2466073/1‑76 (MQ=255) aGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCca < 2:2837198/76‑1 (MQ=255) gCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCcac > 1:5253613/1‑76 (MQ=255) gCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCcac > 2:1004572/1‑76 (MQ=255) catcatAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCcaca > 2:6532444/1‑76 (MQ=255) atcatAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACAt < 1:4493537/76‑1 (MQ=255) tcatAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATc < 1:4406843/76‑1 (MQ=255) tcatAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATc < 1:2245837/76‑1 (MQ=255) catAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCg < 1:1360068/76‑1 (MQ=255) catAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCg > 2:6152360/1‑76 (MQ=255) atAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGc > 1:4142637/1‑76 (MQ=255) tAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGcc > 2:6624991/1‑76 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGccc > 1:4723411/1‑76 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGccc > 2:3570482/1‑76 (MQ=255) aaTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGccc > 2:2981759/1‑76 (MQ=255) aTGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCt > 1:8227709/1‑76 (MQ=255) tGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTg < 1:656740/76‑1 (MQ=255) tGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTg < 2:1851628/76‑1 (MQ=255) tGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTg < 1:6042731/76‑1 (MQ=255) tGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTg < 1:829033/76‑1 (MQ=255) tGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTg < 2:7390147/76‑1 (MQ=255) gCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGtt < 2:4266051/76‑1 (MQ=255) gCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGtt < 2:6223438/76‑1 (MQ=255) gATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTc‑c < 1:7762319/76‑1 (MQ=255) gATAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTc‑c > 1:2158801/1‑76 (MQ=255) aTAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑ca < 1:4168965/76‑1 (MQ=255) tAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑cat < 1:907404/76‑1 (MQ=255) tAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑cat < 1:2754370/76‑1 (MQ=255) tAAATGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑cat < 1:3011465/76‑1 (MQ=255) aaaTGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑catc > 1:7989586/1‑76 (MQ=255) aaaTGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑catc < 2:7538000/76‑1 (MQ=255) aaaTGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑catc > 1:8181458/1‑76 (MQ=255) aaTGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑catca < 1:4880984/76‑1 (MQ=255) aTGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑catcat < 1:6418961/76‑1 (MQ=255) tGCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCAtt > 1:1542801/1‑76 (MQ=255) gCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTc > 2:2144241/1‑76 (MQ=255) gCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTc > 2:8655507/1‑76 (MQ=255) gCGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTc > 2:7128015/1‑76 (MQ=255) cGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTcc > 1:6637595/1‑76 (MQ=255) cGATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTcc > 2:1598293/1‑76 (MQ=255) gATCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCt < 2:4126185/76‑1 (MQ=255) aTCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTg < 2:1145516/76‑1 (MQ=255) aTCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTg < 1:3682938/76‑1 (MQ=255) tCACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGc > 2:6282711/1‑76 (MQ=255) cACTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGcc > 1:4003373/1‑76 (MQ=255) aCTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCa > 2:7618021/1‑76 (MQ=255) aCTTTGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCa > 1:4558449/1‑76 (MQ=255) tttGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACt > 2:235354/1‑76 (MQ=255) tttGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACt > 2:811228/1‑76 (MQ=255) ttGCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTg > 2:5953655/1‑76 (MQ=255) gCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCg > 1:6177425/1‑76 (MQ=255) gCCGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCg > 2:4720786/1‑76 (MQ=255) ccGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCgg > 2:4540247/1‑76 (MQ=255) cGATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTg < 2:5238043/66‑1 (MQ=255) gATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGgc > 1:3380636/1‑76 (MQ=255) gATGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGgc > 1:1255779/1‑76 (MQ=255) aTGGGGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGgca > 1:1717017/1‑76 (MQ=255) tGGTGATCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGgcag > 1:741665/1‑76 (MQ=255) tGACCCCCATAACGTCTGAAAGTGGGGCAACGCCCAC‑GCCCCCGCTTCACATCACCCCTGCCACTGCGGGCAGCaa < 2:1393871/76‑1 (MQ=255) accccaATAACGACGGCAAGAGGGCCAAACCCCACACCCCCCTTTTC‑CATCATCCCTGCCACTGCGGGCAGCAaca < 1:1179925/74‑1 (MQ=255) aTCCCCATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGGCAGCAaca > 1:5479489/1‑76 (MQ=255) ccccATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGGCAGCAACAcc > 2:3155130/1‑76 (MQ=255) ccccATAACGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGGCAGCAACAcc > 1:8599749/1‑76 (MQ=255) ccccAAAACGACGGAAAGAGTGGAAAACCCCACATCGCCCTGTTC‑CATCATCCCTGCCACTGCGGGCAGCAACAcc < 1:5113417/76‑1 (MQ=255) cccATAACGACTGCAAGAGGGGCAAACCCCACATCCCCCTGTCC‑CATCATCCCTGCCACTGCGGGCAGCAACACCa < 1:8102561/76‑1 (MQ=255) cccATAACGACGGCAGGAGGGGCAAAGCCCACCCCGCCCTTTTC‑CATCATTCCTGCCACTGCGGGCAGCAACACCa < 1:8766897/76‑1 (MQ=255) cccAAAACGACGGAAAGAGTGACAAACCCCACCCCCCCCTGTTC‑CATCCTCCCTGCCACTGCGGGCAGCAACACCa < 2:2629862/76‑1 (MQ=255) ataaCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGGCAGCAACACCAGCg > 2:7168492/1‑76 (MQ=255) ataaCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGGCAGCAACACCAGCg > 1:7506546/1‑76 (MQ=255) | CGGCACCAGACGGCGACCTACCAGCATCATAATGGCGATAAATGCGATCACTTTGCCGATGGTGATCCCCATATCGACTGCAAGAGTGGCAAAGCCCACATCGCCCTGTTC‑CATCATTCCTGCCACTGCGGGCAGCAACACCAGCG > REL606/474670‑474815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |