BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	754.7 / NA	190	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (110/80); total (110/80)

Only 100 of 220 total aligned reads displayed.

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

  aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                      >  2:8281012/1‑76 (MQ=255)

  aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                      <  2:6782875/76‑1 (MQ=255)

   gTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATc                                                                     >  1:6757513/1‑76 (MQ=255)

    tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                    >  1:8233679/1‑76 (MQ=255)

    tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                    >  2:4677883/1‑76 (MQ=255)

     ccccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg                                                                   <  1:7989149/76‑1 (MQ=255)

     ccccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg                                                                   <  2:2332302/76‑1 (MQ=255)

      cccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgc                                                                  <  1:2339150/76‑1 (MQ=255)

      cccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTAAGTGGTGAAATCCAGCGTCGGTTACATCcgc                                                                  <  1:426298/76‑1 (MQ=255)

       ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg                                                                 <  1:2950446/76‑1 (MQ=255)

       ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgcg                                                                 >  1:3448228/1‑76 (MQ=255)

         ggCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAt                                                               <  2:1225546/76‑1 (MQ=255)

          gCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGAtt                                                              <  1:3045796/76‑1 (MQ=255)

            aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                            >  1:2167770/1‑76 (MQ=255)

             aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           <  2:8845593/76‑1 (MQ=255)

              aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc                                                          <  1:3667349/76‑1 (MQ=255)

               aGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCg                                                         <  2:4785358/76‑1 (MQ=255)

                 ccAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAc                                                       >  2:5415199/1‑76 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      >  2:3737009/1‑76 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      <  2:4185819/76‑1 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      >  2:8955849/1‑76 (MQ=255)

                  cAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGAcc                                                      >  1:1519814/1‑76 (MQ=255)

                   aaaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCa                                                     >  2:2878197/1‑76 (MQ=255)

                    aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                    <  2:5000613/76‑1 (MQ=255)

                    aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                    >  2:5480122/1‑76 (MQ=255)

                    aaaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAg                                                    >  2:665713/1‑76 (MQ=255)

                     aaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAgg                                                   >  1:8290336/1‑75 (MQ=255)

                     aaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGt                                                   >  2:691244/1‑76 (MQ=255)

                       cGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTgg                                                 >  1:3020013/1‑76 (MQ=255)

                        gATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGc                                                >  2:6454402/1‑76 (MQ=255)

                        gATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGc                                                <  2:8559117/76‑1 (MQ=255)

                         aTCAACAAGTATCTGGGTAGTGACTACGTGTTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCt                                               <  2:4936917/76‑1 (MQ=255)

                          tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                              >  1:4749661/1‑76 (MQ=255)

                          tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                              <  1:3125724/76‑1 (MQ=255)

                           caacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCa                                             <  2:250999/76‑1 (MQ=255)

                            aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                            <  1:6855854/76‑1 (MQ=255)

                             acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGGCCAGGGGCTCAGc                                           >  2:7480472/1‑76 (MQ=255)

                             acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGc                                           >  1:4188046/1‑76 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:1675488/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          >  2:2970404/1‑76 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  2:2263160/76‑1 (MQ=255)

                              caaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCCACCAGTGGCTCAGCt                                          >  2:7636074/1‑76 (MQ=255)

                               aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGTTCAGCTg                                         >  1:1967499/1‑76 (MQ=255)

                               aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGccg                                         >  1:4583817/1‑74 (MQ=255)

                               aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         >  2:3624075/1‑76 (MQ=255)

                               aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         >  2:7138544/1‑76 (MQ=255)

                                aGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGc                                        >  1:1169000/1‑76 (MQ=255)

                                  tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa                                      >  1:5747548/1‑76 (MQ=255)

                                  tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa                                      <  1:6035366/76‑1 (MQ=255)

                                  tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa                                      >  1:8449258/1‑76 (MQ=255)

                                  tATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCACCTGccc                                      >  2:598086/1‑75 (MQ=255)

                                   aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                     <  1:4159000/76‑1 (MQ=255)

                                     cTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCCGCTGCCcaaa                                   >  1:5453631/1‑76 (MQ=255)

                                     cTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaa                                   >  1:2154119/1‑76 (MQ=255)

                                      tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaaa                                  <  1:9001278/76‑1 (MQ=255)

                                      tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaaa                                  >  1:5279166/1‑76 (MQ=255)

                                       gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCCAAAag                                 >  2:3159548/1‑76 (MQ=255)

                                       gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAag                                 <  2:210401/76‑1 (MQ=255)

                                       gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAag                                 <  1:6184859/76‑1 (MQ=255)

                                       gggCAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCACCTGCCCAAAag                                 >  2:6334252/1‑76 (MQ=255)

                                         gTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAagag                               >  2:1941512/1‑76 (MQ=255)

                                          tAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGt                              >  2:8718237/1‑76 (MQ=255)

                                          tAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGt                              >  1:4403004/1‑76 (MQ=255)

                                              gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          <  2:768212/76‑1 (MQ=255)

                                              gACTACGTGGTAAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          <  2:4898626/76‑1 (MQ=255)

                                               actactTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGa                         <  2:8686729/76‑1 (MQ=255)

                                                cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc                        >  1:7397544/1‑76 (MQ=255)

                                                   cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     >  1:2476263/1‑76 (MQ=255)

                                                   cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     >  2:5207624/1‑76 (MQ=255)

                                                   cGTGGTGAAACCCAGCGTCGTTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGActct                     <  2:7318823/76‑1 (MQ=255)

                                                    gtggtgAATTCCAGCGTCGGTTACATCCGCGTTTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  2:1711743/76‑1 (MQ=255)

                                                    gtggtgAAGTCCAGCGTCGTTTACATCCGCGATTTACCGCCCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    <  2:1336198/76‑1 (MQ=255)

                                                    gtggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTa                    >  1:136744/1‑76 (MQ=255)

                                                      ggtgAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTAcc                  >  1:508239/1‑76 (MQ=255)

                                                       gtgAAATCCGGCGTCGGTTACATCCGGGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCt                 <  2:3430528/76‑1 (MQ=255)

                                                         gAAATCCAGCGTCGTTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcc               <  1:1690959/76‑1 (MQ=255)

                                                         gAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcc               <  1:6007281/76‑1 (MQ=255)

                                                         gAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGGGCCGACTCTACCTcc               >  2:3850851/1‑76 (MQ=255)

                                                          aaaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTcca              <  1:1625610/76‑1 (MQ=255)

                                                           aacccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccac             <  2:620425/73‑1 (MQ=255)

                                                           aaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccac             >  2:8086611/1‑76 (MQ=255)

                                                           aaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGGGCCGACTCTACCTccac             >  2:5851973/1‑76 (MQ=255)

                                                            aTCCAGCGTCGGTTACATCCGCGATTTGCCGACTAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            >  1:8886707/1‑76 (MQ=255)

                                                              ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          <  1:8718237/76‑1 (MQ=255)

                                                              ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          <  2:2951480/76‑1 (MQ=255)

                                                               cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         <  1:1612430/76‑1 (MQ=255)

                                                                aGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGa        >  1:4979829/1‑76 (MQ=255)

                                                                aGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGa        <  1:3224537/76‑1 (MQ=255)

                                                                aGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGGGCCGACTCTACCTCCACCCAGa        >  1:8006698/1‑76 (MQ=255)

                                                                  ggTCGGTTCCATCCGGGATTTCCCGCCCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg      <  2:5568164/75‑1 (MQ=255)

                                                                  cGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg      <  1:7774047/76‑1 (MQ=255)

                                                                  cGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg      <  1:4885170/76‑1 (MQ=255)

                                                                    tCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGc    >  2:5718693/1‑76 (MQ=255)

                                                                     cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   >  1:3750231/1‑76 (MQ=255)

                                                                     cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   <  1:8533936/76‑1 (MQ=255)

                                                                      ggTTACATCCGCGATTTGCCGCCCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  1:6711076/76‑1 (MQ=255)

                                                                      ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  2:4244746/76‑1 (MQ=255)

                                                                      ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  1:2051461/76‑1 (MQ=255)

                                                                      ggTTACACCCGCGATTTGCCGCCCGGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  2:1519814/76‑1 (MQ=255)

                                                                      ggTTACACCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  1:7669690/76‑1 (MQ=255)

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`