| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,592,630 | A→T | D31V (GAT→GTT) | ydeJ → | competence damage‑inducible protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,592,630 | 0 | A | T | 97.2% | 1128.1 / 11.5 | 288 | D31V (GAT→GTT) | ydeJ | competence damage‑inducible protein A |
| Reads supporting (aligned to +/- strand): ref base A (4/4); new base T (164/116); total (168/120) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 298 total aligned reads displayed.
AAAATTGTACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGATCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAGCTGAAGA > REL606/1592557‑1592702 | aaaTTGTACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCa < 2:8452971/76‑1 (MQ=255) aaaTTGCACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCa > 1:2175204/1‑76 (MQ=255) aaTTGTACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCaa > 2:5811089/1‑76 (MQ=255) aTTGTACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAAt > 2:5154040/1‑76 (MQ=255) ttGTACAACTTGTAGATACCGATATTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAAtt > 1:1647405/1‑76 (MQ=255) tggaCATCTTTTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGATCAATTa < 1:1013570/73‑1 (MQ=255) tGTACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTa > 1:7341651/1‑76 (MQ=255) gTACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTAc < 2:7952497/76‑1 (MQ=255) gTACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTAc > 1:7616957/1‑76 (MQ=255) tACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACg > 2:4877125/1‑76 (MQ=255) aaCTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCtt > 2:6584750/1‑76 (MQ=255) aCTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTa > 1:3324492/1‑76 (MQ=255) cTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTaa < 2:3969361/76‑1 (MQ=255) cTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTaa < 1:5814282/76‑1 (MQ=255) ttGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAAc < 2:2300174/76‑1 (MQ=255) ttGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAAc > 2:6041087/1‑76 (MQ=255) tGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACt > 1:6830417/1‑76 (MQ=255) tGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACt > 2:7297151/1‑76 (MQ=255) tGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACt < 1:8724027/76‑1 (MQ=255) gTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTa > 1:5623068/1‑76 (MQ=255) tAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTAc > 2:8528318/1‑76 (MQ=255) tAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTAc < 2:5875106/76‑1 (MQ=255) gATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTAccg < 2:7439943/76‑1 (MQ=255) aTACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTAccgc > 1:5450305/1‑76 (MQ=255) aTACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTAccgc > 2:1609889/1‑76 (MQ=255) tACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTAccgcc < 1:311241/76‑1 (MQ=255) aCCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTAccgccg < 2:3540113/76‑1 (MQ=255) cGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGaa > 1:4827673/1‑76 (MQ=255) gATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAAt > 2:5332251/1‑76 (MQ=255) aTACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATc > 1:6516849/1‑76 (MQ=255) tACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCa < 2:3131243/76‑1 (MQ=255) tACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCa < 1:4659953/76‑1 (MQ=255) cTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATg > 1:1836095/1‑76 (MQ=255) cTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATg > 1:4234462/1‑76 (MQ=255) cTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATg > 2:4480131/1‑76 (MQ=255) tATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGc > 2:3493870/1‑76 (MQ=255) tATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGc > 2:8827804/1‑76 (MQ=255) tATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGc > 1:2064552/1‑76 (MQ=255) tATTGAAAACCTGAAATACGCGTTGATTCAAAGCCTTATCGCGGTTGAATTATGCTTCACTAACTCAGAATCATgt > 2:7544773/1‑75 (MQ=255) aTTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCa > 1:6151331/1‑76 (MQ=255) aTTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCa > 1:7804517/1‑76 (MQ=255) ttGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCAc > 2:2069116/1‑76 (MQ=255) ttGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGATCAATTACGCTTAACTACCGCCGAATCATGCAc < 1:3768284/76‑1 (MQ=255) tGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCAcc > 1:6468277/1‑76 (MQ=255) aaaCCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACcggc > 2:2221577/1‑76 (MQ=255) aaaCCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACcggc > 1:7109774/1‑76 (MQ=255) aaCCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACcggcg < 1:7927890/76‑1 (MQ=255) ccTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGt < 2:1406455/76‑1 (MQ=255) cTGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTa < 2:6439657/76‑1 (MQ=255) tGACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTaa < 2:7649859/76‑1 (MQ=255) gACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTACCTCCCGCCGAATCATGCACCGGCGGTAAg < 1:4524745/76‑1 (MQ=255) gACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAg > 1:8499727/1‑76 (MQ=255) gACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAg < 2:8658533/76‑1 (MQ=255) gACATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGGAAg > 2:1008399/1‑76 (MQ=255) aCATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGt > 1:1114143/1‑76 (MQ=255) aCATCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGt < 2:1743590/76‑1 (MQ=255) aTCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTg < 2:8363768/76‑1 (MQ=255) aTCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTg > 1:4735934/1‑76 (MQ=255) aTCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGGAAGTTg > 1:6303378/1‑76 (MQ=255) tCCGCGTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTATCGCCGAATCATGCACCGGCGGTAAGTTgg < 2:2572684/76‑1 (MQ=255) cgcgTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGTCt < 1:6714507/76‑1 (MQ=255) cgcgTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCt < 2:34054/76‑1 (MQ=255) cgcgTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCt < 2:3624044/76‑1 (MQ=255) cgcgTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCt < 2:429869/76‑1 (MQ=255) gcgTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTa < 1:4992658/76‑1 (MQ=255) gcgTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTa < 2:5207036/76‑1 (MQ=255) cgTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAg < 1:140990/76‑1 (MQ=255) gTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAgc > 1:2718570/1‑76 (MQ=255) gTTGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAgc < 2:1916299/76‑1 (MQ=255) ttGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGCTGGCTAgcg > 2:9012917/1‑76 (MQ=255) tGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAgcgc > 2:8036824/1‑76 (MQ=255) tGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAgcgc > 2:7099135/1‑76 (MQ=255) tGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAgcgc > 2:8180949/1‑76 (MQ=255) tGAGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAgcgc < 1:2422368/76‑1 (MQ=255) aGTCAAAGTCTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGccc < 2:7379806/76‑1 (MQ=255) aGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAgc < 1:5142830/72‑1 (MQ=255) aGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGccc > 1:2385643/1‑76 (MQ=255) aGTCAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAAGGccc > 1:3530025/1‑76 (MQ=255) cAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCtgt > 2:8203385/1‑76 (MQ=255) cAAAGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCcgt > 2:5135005/1‑76 (MQ=255) aaaGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCtgtg > 1:4000360/1‑76 (MQ=255) aGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTATCGCCCtgcgtg > 1:1547637/1‑76 (MQ=255) aGACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCtgtgtg < 2:4156190/76‑1 (MQ=255) gACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGc < 2:4674799/76‑1 (MQ=255) gACTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGc > 2:953992/1‑76 (MQ=255) aCTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCa > 2:6090617/1‑76 (MQ=255) aCTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCa < 1:8117383/76‑1 (MQ=255) aCTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCa < 2:1242668/76‑1 (MQ=255) cTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAg < 1:7854949/76‑1 (MQ=255) cTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAg < 2:8316797/76‑1 (MQ=255) cTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCCGTGGGCAg > 2:2436468/1‑76 (MQ=255) cTTATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAACGCCCCGGGTGCAg > 2:553726/1‑76 (MQ=255) ttATCGGGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAGc < 2:7832531/76‑1 (MQ=255) ttATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGGAGc > 2:2993644/1‑76 (MQ=255) ttATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAGc > 2:2763339/1‑76 (MQ=255) ttATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAGc > 2:1110660/1‑76 (MQ=255) tATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAGCt < 1:6041087/76‑1 (MQ=255) tATCGCGGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAGCt < 2:8996082/76‑1 (MQ=255) cgcgGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAGCTGaa < 2:8123308/76‑1 (MQ=255) cgcgGTTCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAGCTGaa > 2:3857161/1‑76 (MQ=255) | AAAATTGTACAACTTGTAGATACCGATACTATTGAAAACCTGACATCCGCGTTGAGTCAAAGACTTATCGCGGATCAATTACGCTTAACTACCGCCGAATCATGCACCGGCGGTAAGTTGGCTAGCGCCCTGTGTGCAGCTGAAGA > REL606/1592557‑1592702 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |