Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,193,7790GT100.0% 788.1 / NA 209L143I (CTT→ATT) yeiBconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (97/112);  total (97/112)

Only 100 of 234 total aligned reads displayed.

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851
                                                                          |                                                                        
gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATcc                                                                        <  2:114675/76‑1 (MQ=255)
 ccGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAACCCCAATAAAAGC‑AAAACGCCCAAccc                                                                       >  1:6675929/1‑76 (MQ=255)
 ccGATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGGAAACAACCCCCAAAAAAGC‑CAAAAGCCCAAccc                                                                       >  1:675995/1‑76 (MQ=255)
  cGATGCATCCGGCGTCCAGGCGCGGCTGGTTTGGCTTTCGGGAAACAACCCCCATAAAAGC‑AAAAAACCAAAcccc                                                                      >  1:5342962/1‑76 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCcca                                                                     <  1:7611896/76‑1 (MQ=255)
   gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCcca                                                                     <  2:1665194/76‑1 (MQ=255)
    aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCccac                                                                    <  1:1288261/76‑1 (MQ=255)
     tGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCCATAAAAGCAAAAACCCCAACcccccc                                                                    >  2:5366726/1‑74 (MQ=255)
      gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCccacca                                                                  <  1:107412/76‑1 (MQ=255)
       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAg                                                                 <  1:1395564/76‑1 (MQ=255)
       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAg                                                                 <  1:1681208/76‑1 (MQ=255)
       cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAg                                                                 <  2:2093998/76‑1 (MQ=255)
        aTCCGGCGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAAACAGCCCCCATAACAGC‑AAAACGCCCAACCCCCCCCGa                                                                >  1:820831/1‑76 (MQ=255)
          ccGGCGTCCAGGCGCGGCTGGGCTGGTTGTCGGGAATCAACCCCCATAAAAGC‑AAAACGCCAATCCCCACCCGATa                                                              >  1:1922341/1‑76 (MQ=255)
           cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaa                                                             <  1:6002784/76‑1 (MQ=255)
            ggCGTCCATGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATcaa                                                            >  1:4329569/1‑76 (MQ=255)
            ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaaa                                                            <  1:8248398/76‑1 (MQ=255)
            ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATaaa                                                            <  2:4252022/76‑1 (MQ=255)
             gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAg                                                           <  1:4727003/76‑1 (MQ=255)
              cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:2568590/76‑1 (MQ=255)
              cGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGc                                                          >  1:6888959/1‑76 (MQ=255)
              cGTCCAGGCGCGGCTGGGCTGGCTGTCGGGAATCAGCCCCAATAAAAGC‑AAAACGCCAATCCCCACCCGATAAAGc                                                          >  1:48310/1‑76 (MQ=255)
               gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCa                                                         <  2:185435/76‑1 (MQ=255)
                 ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATg                                                       <  1:6427014/76‑1 (MQ=255)
                       gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                 <  1:2732025/76‑1 (MQ=255)
                        cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  1:6251311/76‑1 (MQ=255)
                         ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  1:4384763/76‑1 (MQ=255)
                         ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  2:6961508/76‑1 (MQ=255)
                          gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              >  2:7709337/1‑76 (MQ=255)
                          gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              >  1:1972170/1‑76 (MQ=255)
                          gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              >  2:4361960/1‑76 (MQ=255)
                          gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                              <  1:8000582/76‑1 (MQ=255)
                           cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  2:6041097/1‑76 (MQ=255)
                           cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAt                                             >  2:6233076/1‑76 (MQ=255)
                            tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:3752565/76‑1 (MQ=255)
                            tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  1:5429289/1‑76 (MQ=255)
                            tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  1:3022015/76‑1 (MQ=255)
                            tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:5643866/1‑76 (MQ=255)
                            tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            <  2:7222875/76‑1 (MQ=255)
                             ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:3116177/1‑76 (MQ=255)
                             ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  1:3541984/76‑1 (MQ=255)
                             ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           <  1:8982417/76‑1 (MQ=255)
                               tCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         <  1:1103040/76‑1 (MQ=255)
                               tCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  2:7071468/1‑76 (MQ=255)
                               tCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  2:5549464/1‑76 (MQ=255)
                               tCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  2:6667206/1‑76 (MQ=255)
                                cTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:5503543/1‑76 (MQ=255)
                                cTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:3045706/76‑1 (MQ=255)
                                cTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:7449687/1‑76 (MQ=255)
                                cTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:6305397/76‑1 (MQ=255)
                                cTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:23967/1‑76 (MQ=255)
                                 tGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                       <  2:7944476/76‑1 (MQ=255)
                                   gCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAgg                                     <  2:6770730/76‑1 (MQ=255)
                                    cTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  1:7816499/1‑76 (MQ=255)
                                    cTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    <  2:3912460/76‑1 (MQ=255)
                                    cTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  1:6391849/1‑76 (MQ=255)
                                    cTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    >  1:8592048/1‑76 (MQ=255)
                                    cTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAAAGGc                                    <  2:5346402/76‑1 (MQ=255)
                                     tGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  2:4536674/1‑76 (MQ=255)
                                     tGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   <  2:3367821/76‑1 (MQ=255)
                                     tGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                   >  2:4942309/1‑76 (MQ=255)
                                      gTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  <  2:8296663/76‑1 (MQ=255)
                                      gTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  1:8462952/1‑76 (MQ=255)
                                        cGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  2:6925648/1‑76 (MQ=255)
                                        cGGAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  1:3624327/76‑1 (MQ=255)
                                         ggAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:6566464/76‑1 (MQ=255)
                                          gAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              >  2:5255745/1‑76 (MQ=255)
                                          gAAATCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              >  1:8030367/1‑76 (MQ=255)
                                           aaaTCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:5078269/1‑76 (MQ=255)
                                            aaTCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                            <  1:7255007/76‑1 (MQ=255)
                                             agcAGCCCCAATAACAGC‑AAAACGCCAGTCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCGTTTTACCg                           <  2:3950489/74‑1 (MQ=255)
                                             aTCAGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCg                           >  1:3221876/1‑76 (MQ=255)
                                                aGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  1:4938169/76‑1 (MQ=255)
                                                aGCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:4773640/76‑1 (MQ=255)
                                                 gCCCCAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATgg                       <  1:5749801/76‑1 (MQ=255)
                                                   cccAATAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     >  2:778583/1‑76 (MQ=255)
                                                      aaTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  2:1564715/1‑76 (MQ=255)
                                                      aaTAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  <  2:2183025/76‑1 (MQ=255)
                                                        tAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:3688245/1‑76 (MQ=255)
                                                        tAACAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                <  2:7028497/76‑1 (MQ=255)
                                                         aaCAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  2:1333000/1‑76 (MQ=255)
                                                         aaCAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  1:4518215/1‑76 (MQ=255)
                                                         aaCAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  2:2353960/1‑76 (MQ=255)
                                                          aCAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              >  1:4044634/1‑76 (MQ=255)
                                                           cAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:227504/76‑1 (MQ=255)
                                                           cAGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             >  1:7088399/1‑76 (MQ=255)
                                                            aGC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCa            >  1:1423360/1‑76 (MQ=255)
                                                             gC‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  1:4282622/76‑1 (MQ=255)
                                                              c‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc          >  2:3961460/1‑76 (MQ=255)
                                                              c‑AAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc          >  1:3508259/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  1:2179270/1‑76 (MQ=255)
                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:8045595/1‑76 (MQ=255)
                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCGTCGCGCACCAg        >  1:3408778/1‑76 (MQ=255)
                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:7596303/76‑1 (MQ=255)
                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        >  2:2442246/1‑76 (MQ=255)
                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:3427291/76‑1 (MQ=255)
                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        <  2:4115451/76‑1 (MQ=255)
                                                                   aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc      <  2:8751772/76‑1 (MQ=255)
                                                                    cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg     >  2:8631359/1‑76 (MQ=255)
                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   >  2:1129552/1‑76 (MQ=255)
                                                                          |                                                                        
GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGC‑AAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑