Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,539,996 T→G I329L (ATC→CTC)  uraA ← uracil transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,539,9960TG99.5% 768.6 / ‑3.6 182I329L (ATC→CTC) uraAuracil transporter
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (93/88);  total (93/89)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

Only 100 of 198 total aligned reads displayed.

AAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATA  >  REL606/2539923‑2540066
                                                                         |                                                                      
aaaaCACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGagca                                                                      <  1:4933513/76‑1 (MQ=255)
 aaaCACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGagcat                                                                     >  2:3647521/1‑76 (MQ=255)
 aaaCACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGagcat                                                                     >  1:4075145/1‑76 (MQ=255)
 aaaCACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGagcat                                                                     <  2:8864754/76‑1 (MQ=255)
 aaaCACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGagcat                                                                     >  1:5130638/1‑76 (MQ=255)
   acacGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCt                                                                   <  1:1080454/76‑1 (MQ=255)
   acacGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCt                                                                   <  1:3944979/76‑1 (MQ=255)
   acacGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCt                                                                   <  1:8743735/76‑1 (MQ=255)
   acacGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCt                                                                   <  1:5427488/76‑1 (MQ=255)
     acGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTgg                                                                 >  1:5235029/1‑76 (MQ=255)
     acGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTgg                                                                 <  1:716566/76‑1 (MQ=255)
     acGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTgg                                                                 >  2:4730513/1‑76 (MQ=255)
       gAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGAt                                                               <  1:159487/76‑1 (MQ=255)
       gAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGAt                                                               >  2:5014222/1‑76 (MQ=255)
        aaTACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATa                                                              <  1:4610853/76‑1 (MQ=255)
        aaTACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATa                                                              >  1:2795808/1‑76 (MQ=255)
         aTACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAg                                                             <  2:1324539/76‑1 (MQ=255)
         aTACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAg                                                             <  1:6661038/76‑1 (MQ=255)
         aTACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAg                                                             <  1:1348348/76‑1 (MQ=255)
         aTACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCACCTGGATAg                                                             >  1:2238750/1‑76 (MQ=255)
          tACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGc                                                            >  2:4643133/1‑76 (MQ=255)
           aCCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCg                                                           >  2:3780118/1‑76 (MQ=255)
            ccGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCgg                                                          >  2:2854861/1‑76 (MQ=255)
             cGGTAGCACCGATGACACCATAAAGCAGCAGCGAATCGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGc                                                         >  2:6771181/1‑76 (MQ=255)
             cGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGc                                                         <  2:5356780/76‑1 (MQ=255)
             cGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGc                                                         >  2:6485168/1‑76 (MQ=255)
             cGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGc                                                         <  2:6580597/76‑1 (MQ=255)
             cGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGc                                                         >  1:8251672/1‑76 (MQ=255)
               gAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAg                                                       <  1:895286/76‑1 (MQ=255)
               gAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAg                                                       <  2:1199244/76‑1 (MQ=255)
                 aGCCCCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGcc                                                     <  2:7904395/76‑1 (MQ=255)
                 aGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAggc                                                     >  2:6905254/1‑74 (MQ=255)
                   cACGGAGGACACCACAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAg                                                   <  2:8547910/76‑1 (MQ=255)
                     ccGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGtt                                                 >  1:3096243/1‑76 (MQ=255)
                     ccGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGtt                                                 <  2:8902004/76‑1 (MQ=255)
                      cGATGACACCATATAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGttt                                                <  1:331123/76‑1 (MQ=255)
                      cGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGGCAGgtt                                                >  1:7450612/1‑76 (MQ=255)
                      cGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCCGttt                                                >  2:91042/1‑76 (MQ=255)
                      cGATGACACCAAAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGttt                                                <  2:5398964/76‑1 (MQ=255)
                       gATGACACCAAAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTa                                               <  1:4482006/76‑1 (MQ=255)
                        aTGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTAc                                              <  1:361243/76‑1 (MQ=255)
                         tGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGGCCGTTTAcc                                             >  1:8378892/1‑76 (MQ=255)
                         tGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGGCAGTTTaac                                             >  1:3724764/1‑74 (MQ=255)
                         tGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCCGTTTAcc                                             >  2:1151007/1‑76 (MQ=255)
                         tGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCCGTTTAcc                                             >  1:4812316/1‑76 (MQ=255)
                         tGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTAcc                                             <  1:2729904/76‑1 (MQ=255)
                         tGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGGTTAcc                                             >  2:6951085/1‑76 (MQ=255)
                         tGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGGTTAcc                                             >  2:4010229/1‑76 (MQ=255)
                          gACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCg                                            <  1:5992783/76‑1 (MQ=255)
                          gACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCg                                            <  1:465233/76‑1 (MQ=255)
                          gACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCg                                            <  1:5069377/76‑1 (MQ=255)
                          gACACCATAAAGCACCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCg                                            <  1:5860991/76‑1 (MQ=255)
                           acacCAAAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGa                                           <  2:7865823/76‑1 (MQ=255)
                             acCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCCGTTTACCGACg                                         >  1:8565443/1‑76 (MQ=255)
                              ccATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGc                                        <  2:6881824/76‑1 (MQ=255)
                                 tAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGGCAGTTTTCCGACGCAgg                                     >  1:2625206/1‑76 (MQ=255)
                                 tAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCCAGTTACCGACGCCgg                                     >  2:2963113/1‑76 (MQ=255)
                                  aaaGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCCGGTTACCGACGCAGGa                                    >  1:3701550/1‑76 (MQ=255)
                                  aaaGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCCGGTTAACGACGCAGGa                                    >  2:822678/1‑76 (MQ=255)
                                   aaGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGaa                                   <  1:6186454/76‑1 (MQ=255)
                                    agcagcagcGAAACGCCGCCCATCCCCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGaaa                                  <  2:6250847/76‑1 (MQ=255)
                                     gcagcagcGAAACGCCGCCCATCCCCGGCAAGGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAg                                 <  2:7530938/76‑1 (MQ=255)
                                     gcagcagcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAg                                 <  2:5594230/76‑1 (MQ=255)
                                      cagcagcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGc                                <  1:8582908/76‑1 (MQ=255)
                                       agcagcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAgaa                               >  2:6330997/1‑74 (MQ=255)
                                       agcagcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCa                               <  1:4266164/76‑1 (MQ=255)
                                         cagcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAgg                             <  1:7757800/76‑1 (MQ=255)
                                          agcGAACCGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGGGGCAGCCAGTTTACCGACGCAAGAAAGCAGGa                            <  1:1031799/76‑1 (MQ=255)
                                          agcGAAACGCCGCCCATCCCCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGa                            <  2:2148938/76‑1 (MQ=255)
                                          agcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGa                            <  1:7378230/76‑1 (MQ=255)
                                          agcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGa                            >  2:1002547/1‑76 (MQ=255)
                                          agcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGa                            <  2:7444002/76‑1 (MQ=255)
                                          agcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGa                            >  2:1888414/1‑76 (MQ=255)
                                          agcGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAACAGGa                            >  1:3205269/1‑76 (MQ=255)
                                            cGAACCCCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATa                          <  1:6866885/76‑1 (MQ=255)
                                            cGAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATa                          >  1:3238766/1‑76 (MQ=255)
                                             gAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGGAAGCAGGATaa                         >  1:2975796/1‑75 (MQ=255)
                                             gAAACGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAg                         <  2:756710/76‑1 (MQ=255)
                                              aaaCGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCCACGCAGGGAAGCAGGATaag                        >  2:5523573/1‑74 (MQ=255)
                                               aaCGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCg                       <  1:2330549/76‑1 (MQ=255)
                                                aCGCCGCCCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGGCAGGTTTCCCACGCGGGAAAGCAGGAAAgggg                      >  2:3767636/1‑73 (MQ=255)
                                                  gccgccCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCCGTTTTACGATGCAGGAAAGCAGGATAGCGaaa                    >  2:6117751/1‑76 (MQ=255)
                                                  gccgccCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGaaa                    <  1:8008648/76‑1 (MQ=255)
                                                   ccgccCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGaaaa                   <  1:1225072/76‑1 (MQ=255)
                                                   ccgccCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGAAGGATAGCGaaaa                   >  1:4386859/1‑76 (MQ=255)
                                                    cgccCATCACCGGCAATGGGAGCATCTGGATAGCGGCAGGCCGGTTACCGACGCAGGAAACGAGGATAGAGgaaaa                  >  2:4817737/1‑75 (MQ=255)
                                                    cccccATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAAt                  <  2:1500444/74‑1 (MQ=255)
                                                       ccATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAATcgc               <  1:3388266/76‑1 (MQ=255)
                                                       ccATCACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAATcgc               <  2:4287252/76‑1 (MQ=255)
                                                           cACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGGTTACCGACGCAGGGAAGCAGGATAGAGAAAAACgccccc           >  1:2402060/1‑76 (MQ=255)
                                                           cACCGGCAATGGGAGCATCTGGATAGCGGCAGCCAGGTTACCGACGCAGGAAAGCAGGATAACGAAAAACgccgcc           >  1:7827135/1‑76 (MQ=255)
                                                             ccGGCAATGGGAGCATCTGGATAGCGGCAGCCCGTTTACCGACGCCGGAAAGCAGGATAACGAAAAAAGccgcccc         >  2:1551231/1‑76 (MQ=255)
                                                              cGGCAATGGGAGCAGCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAATCGccgccccg        <  1:2175393/76‑1 (MQ=255)
                                                               gacAATGGGAGCATCTGGATGGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAATCGccgccccgc       <  2:164964/74‑1 (MQ=255)
                                                                 cAATGGGAGCATCTGGATAGCGGCAGGCAGGTTACCGACGCAGGAAAGCAGGATAGAGAAAAAAGCCGCCcccccg     >  2:7343734/1‑76 (MQ=255)
                                                                 cAATGGGAGCATCTGGACAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCccgccg     >  1:942298/1‑76 (MQ=255)
                                                                  aaTGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGGTAGAGAAAATCGCCGCCCCGCCGa    >  1:2021481/1‑76 (MQ=255)
                                                                  aaTGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAAGGAAAAACGCCCCCcccccgc    >  2:4965404/1‑75 (MQ=255)
                                                                    tGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAAACGCCCCCCCCCCGATa  >  1:5773581/1‑76 (MQ=255)
                                                                    tGGGAGCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAAAAGCCGCCCCCCCGATa  >  1:7524527/1‑76 (MQ=255)
                                                                         |                                                                      
AAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATAGCGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATA  >  REL606/2539923‑2540066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑