| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,144,295 | 0 | A | C | 43.2% | 309.7 / 122.3 | 220 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (101/24); new base C (0/95); total (101/119) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-39 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 259 total aligned reads displayed.
CGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGACG > REL606/2144220‑2144369 | cGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCa < 2:1386762/76‑1 (MQ=255)cGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCa < 2:5229736/76‑1 (MQ=255)cGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCa > 2:2344501/1‑76 (MQ=255) aTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCAcc > 2:6951221/1‑76 (MQ=255) aTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCAcc > 1:1626640/1‑76 (MQ=255) gTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCt < 1:9153728/76‑1 (MQ=255) gTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCt > 1:852934/1‑76 (MQ=255) tATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTg < 1:1646125/76‑1 (MQ=255) aTGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGc > 1:2127050/1‑76 (MQ=255) aTGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGc > 1:367831/1‑76 (MQ=255) tGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGcc < 1:8854038/76‑1 (MQ=255) tGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGcc < 1:7687652/76‑1 (MQ=255) gaagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCa < 1:7514890/76‑1 (MQ=255) aagaaTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAg < 2:2978347/76‑1 (MQ=255) aTAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTaga < 1:5871736/76‑1 (MQ=255) ccAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAgagctg < 2:3679163/76‑1 (MQ=255) ccAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAgagctg > 2:4966642/1‑76 (MQ=255) ccAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAgagctg > 2:9514434/1‑76 (MQ=255) ccAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAgagctg > 2:5836964/1‑76 (MQ=255) ccAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCGGGAATGACTTCCCCCTGCCAGTAgagctg < 2:3426798/76‑1 (MQ=255) aGAATGGTTAATAGGCGTTAGCCCGCGCGGAAGGAAATCTCGCCTGGAATGCCTTCCCCCTGCCAGTAgagctgag < 2:5680554/76‑1 (MQ=255) aGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAACTCTCGCCTGGAATGCCTTCCCCCTGCCAGTAgagctgag < 2:1759636/76‑1 (MQ=255) aGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAgagctgag > 1:9339336/1‑76 (MQ=255) aGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAgagctgag > 1:6105193/1‑76 (MQ=255) aGAATGGTTAATAGGCGTTAGACCGCGCGGAAGAAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAgagctgag < 2:9485034/76‑1 (MQ=255) aaTGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAgagctgagct < 2:1569138/76‑1 (MQ=255) aTGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGagctgagctg < 1:4500799/76‑1 (MQ=255) aTGGTTAATAGGCGTAAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGagctgagctg < 1:2491012/76‑1 (MQ=255) tGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGc > 2:5337176/1‑76 (MQ=255) ttAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCt < 1:9382112/54‑1 (MQ=255) ttAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAc > 2:321838/1‑76 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 2:598043/1‑76 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 2:9187124/1‑76 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 2:8073446/1‑76 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 2:6430437/1‑76 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 2:2373554/1‑76 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 2:3263897/1‑76 (MQ=255) aaTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgc > 2:4259812/1‑76 (MQ=255) aTAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAAcgcg > 1:4102860/1‑76 (MQ=255) tAGGCGTCAGCCCGCGCGGAAGGAAAGCTCCCCTGGAATGTCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCgg < 2:1927907/76‑1 (MQ=255) aGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 2:2568820/1‑76 (MQ=255) aGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:8375448/1‑76 (MQ=255) aGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:7661391/1‑76 (MQ=255) ggCGTTAGCCCGCGCGGAAGGAAATCTCGCCTGGAATGCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 1:6370414/76‑1 (MQ=255) ggCGTTAGCCCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 1:5373972/76‑1 (MQ=255) ggCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 1:5552390/76‑1 (MQ=255) ggCGTTAGACCGCGCGGAAGAAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTc < 1:1936034/76‑1 (MQ=255) gCGTTAGGCCGCGCGGAAGAAAACCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTca < 2:2140083/76‑1 (MQ=255) cGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCCGTATAGCTGAGCTGCAACGCGGTcag > 1:10508/1‑76 (MQ=255) cGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcag > 1:5299582/1‑76 (MQ=255) gTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagc > 1:4521122/1‑76 (MQ=255) ttAGGCCGCGGGGAAGAAAATCTTCCCGGTAATGCTTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcc < 1:181293/76‑1 (MQ=255) ttAGCCCGCGCGGAAGAAAATCTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcc < 2:1554643/76‑1 (MQ=255) ttAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcc < 2:2695013/76‑1 (MQ=255) ttAGACCGCGCGGAAGGAAATCTCGCCGGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcc < 2:865521/76‑1 (MQ=255) ttAGACCGCGCGGAAGAAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcc < 1:6924862/76‑1 (MQ=255) tAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcca > 2:5102407/1‑76 (MQ=255) tAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagcca > 2:7207745/1‑76 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTcagccag > 1:3072433/1‑76 (MQ=255) ccGGGCGGAAGAAAATCTCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTg < 2:1734504/76‑1 (MQ=255) gcgggGAGGGAAATCACGCCTGGAAGGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGc < 2:8847233/76‑1 (MQ=255) gcgcgGAAGGAAATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGc < 2:2056966/76‑1 (MQ=255) ggcgAAAGGAAGCCTCACCTGGAATGCTTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCg < 2:859815/75‑1 (MQ=255) cgcgGAGGAAAATCTCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCg < 2:6196065/76‑1 (MQ=255) cgcgGAAGGAAATCTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCg < 2:5900694/76‑1 (MQ=255) cgcgGAAGAAAACCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCg < 2:747205/76‑1 (MQ=255) cgcgAAAGGAAATCTCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCg < 2:5296311/76‑1 (MQ=255) gcgGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCgg > 2:3478295/1‑76 (MQ=255) ggaaagaaaACCTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGt < 2:1034359/73‑1 (MQ=255) ggaaggaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTa > 2:7963105/1‑76 (MQ=255) gaagaaaATCGCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAg < 1:4416445/76‑1 (MQ=255) aaggaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGa > 1:1223990/1‑76 (MQ=255) aaggaaATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGa > 1:7416069/1‑76 (MQ=255) aagaaaACCTCCCCTGAAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGa < 1:3989204/76‑1 (MQ=255) aggaaATCTCGCCGGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGAt < 2:7817136/76‑1 (MQ=255) agaaaaCCTCCCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGAt < 2:6878026/73‑1 (MQ=255) ggaaATCTCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATa < 1:4772002/76‑1 (MQ=255) gaaTTCTCGCGTGGAATGCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 2:4002354/76‑1 (MQ=255) gaaGTCGCGCCTAAAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 2:6056672/76‑1 (MQ=255) gaaGTCCCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 2:4832368/76‑1 (MQ=255) gaaATCTCGCCTGGAATGCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 2:92618/76‑1 (MQ=255) gaaATCTCGCCTGGAATGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 2:8776225/76‑1 (MQ=255) gaaACCGCGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 2:4725343/76‑1 (MQ=255) aaaaTCTCGCCTGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 2:7452890/75‑1 (MQ=255) aaaaTCTCGCCGGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAg < 2:9403973/75‑1 (MQ=255) aacctcGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCg < 2:7100235/73‑1 (MQ=255) aaTCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCg > 1:6618875/1‑76 (MQ=255) aaTCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCg > 1:3614328/1‑76 (MQ=255) cgcgTGGAATCCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTaaa < 2:6105193/76‑1 (MQ=255) cGCCTGGAATGACTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTaaa < 2:9339336/76‑1 (MQ=255) ccTGGAATGCCTTCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAAtt < 2:7913705/76‑1 (MQ=255) tGGAATGCCTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCg < 1:5067105/76‑1 (MQ=255) ggAATGACTTCACCCTGCCAGTAGGGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGc > 2:8521506/1‑76 (MQ=255) ggAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGc > 2:4641784/1‑76 (MQ=255) ggAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGc > 1:2868196/1‑76 (MQ=255) aTGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGctct > 2:4397686/1‑76 (MQ=255) aTGACTCCCCCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGctct < 2:4439703/76‑1 (MQ=255) gACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTcc > 1:2720760/1‑76 (MQ=255) tCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGAc > 2:7695930/1‑76 (MQ=255) tCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGAc > 1:3289622/1‑76 (MQ=255) | CGATGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCTGCCAGTAGAGCTGAGCTGCAACGCGGTCAGCCAGCTGGCGGTAGATAGCGGTAAATTCGCTCTCCGGACG > REL606/2144220‑2144369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |