Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,304,4070CA41.4% 150.1 / 135.4 116Q266K (CAA→AAA) yfaDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (42/26);  new base A (0/48);  total (42/74)
Fisher's exact test for biased strand distribution p-value = 6.33e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 124 total aligned reads displayed.

AGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAA  >  REL606/2304333‑2304482
                                                                          |                                                                           
agagaTTAATTGCTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACa                                                                            <  1:2905131/76‑1 (MQ=255)
agagaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACa                                                                            <  2:862307/76‑1 (MQ=255)
agagaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACa                                                                            <  2:4707865/76‑1 (MQ=255)
agagaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACa                                                                            <  2:5294172/76‑1 (MQ=255)
  agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAg                                                                          <  1:606422/76‑1 (MQ=255)
  agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAg                                                                          <  1:6848657/76‑1 (MQ=255)
  agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAg                                                                          <  1:6705233/76‑1 (MQ=255)
  agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAg                                                                          <  1:2708887/76‑1 (MQ=255)
  agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAg                                                                          <  1:2645875/76‑1 (MQ=255)
  agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAg                                                                          <  1:2548825/76‑1 (MQ=255)
  agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAg                                                                          <  2:5469325/76‑1 (MQ=255)
  agaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAg                                                                          <  2:671285/76‑1 (MQ=255)
   gaTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGc                                                                         <  2:717547/76‑1 (MQ=255)
    aTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGcc                                                                        <  2:7857399/76‑1 (MQ=255)
    aTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGcc                                                                        <  1:6437509/76‑1 (MQ=255)
    aTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGcc                                                                        <  2:6176473/76‑1 (MQ=255)
    aTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGcc                                                                        <  2:3698881/76‑1 (MQ=255)
    aTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGcc                                                                        <  2:3165256/76‑1 (MQ=255)
    aTTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGcc                                                                        <  2:1278114/76‑1 (MQ=255)
                tGCTGAACGGCTAGGGAAGGAAGGCAATAAAGTGGGCGGGAAGGAAGGTATGAAAAAAAAAGCCATTAAAATTGCt                                                            <  2:3596467/76‑1 (MQ=255)
                  cTGACCGGCTAGGGAAGGAAGGGCATAAAATGGGCGGGAAGGAAGTTATGCAAAAAAAAGCCATTAAAATTGCttt                                                          <  1:8285839/76‑1 (MQ=255)
                       cGGCTAGGGCAGGAGGGGCATAAAATTGGCGGGGAGGAAGGTATGAATAAAAAAGCCATTAAAATTGCTTTGCGCa                                                     <  1:2249253/76‑1 (MQ=255)
                       cGGCTACGGAAGGAAGGGCATAAATTGGGCGGGAAGAAAGGTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCa                                                     <  1:2997286/76‑1 (MQ=255)
                       cGGCAACGGCAGGAAGGGCATAAAATGGGCGGGAAGGAAGTTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCa                                                     <  1:1518168/76‑1 (MQ=255)
                          ctgcGGAAGGAGGGGCAACAAGTTGGCGGGAAGGAAGGTATGCATAAAAAAGCCATTAAAATTGCTTTGCGCATGc                                                  <  1:2949387/73‑1 (MQ=255)
                           tACGGCAGGAAGGGCTTCAAATTGGCGGGAAGAAAGGTATGCAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCt                                                 <  1:5807117/76‑1 (MQ=255)
                           tACGGCAGGAAGGGCATCAAAGGGGCTGGAAGGAAGTTATGCAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCt                                                 <  1:1514530/76‑1 (MQ=255)
                             cGGCAGGAAGGGCATCAAATTGGCTGGCAGGGAGGGAAGGATGAACAAAGCCTTAAAAATTCTTTTCGGCTGCTgg                                               >  2:7965430/1‑76 (MQ=255)
                              ggaaggaggGGCATAAAATGGGGGGGAAGAAAGGTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGa                                              <  2:6786810/73‑1 (MQ=255)
                              ggaaGGAAGGGCATAAAATGGGCGGGAAGGAAGTTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGa                                              <  1:377871/73‑1 (MQ=255)
                              ggCAGGAGGGGCATCAAATGGGCGGGAAGGAAGGTATGCAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGa                                              <  1:1597613/76‑1 (MQ=255)
                              ggCAGGAGGGGCATAAAGTTGGGGGGGAGGAAGGTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGa                                              <  1:4968588/76‑1 (MQ=255)
                              ggCAGGAAGGGAATAAAATGGGCTGGCAGGAAGGTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGa                                              <  1:8847942/76‑1 (MQ=255)
                               gaaGGAAGGGCATCAATTGGGCTGGAAGGAAGGTATGCAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGaa                                             <  2:3500043/74‑1 (MQ=255)
                               gCAGGAGGGACATAAAATTGGCTGGGAGAAAGGTAAGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGaa                                             <  2:6269084/76‑1 (MQ=255)
                               agaGGAAGGTAATAAAATTGGGTGGAAGGAAGGTATAAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGaa                                             <  2:4795489/74‑1 (MQ=255)
                                 aGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCCTGCTGGAACa                                           >  1:513704/1‑76 (MQ=255)
                                 aGGAAGGGCATCAAATTGGCTGGCAGGAAGGGATGCATGAACAAGCCCTTAAAATTGCTTTGCGCCTGCTGGAACa                                           >  1:3494450/1‑76 (MQ=255)
                                 aGGAAGGGCATAAAATTGGCTGGAAGGAAGGTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACa                                           <  1:6966545/76‑1 (MQ=255)
                                 aGGAAGGGCAAAAAATGGGCGGGAAGAAAGGAATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACa                                           <  1:908320/76‑1 (MQ=255)
                                   gaggggAATCAAGTGGGGGGGAAGGAAGTTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAgg                                         <  1:6242065/73‑1 (MQ=255)
                                   gAAGGGCATAAAGTGGGCGGGCAGGAAGTTAGAAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAgg                                         <  1:5961317/76‑1 (MQ=255)
                                   gAAGGGAATCAATTGGGGGGGAAGGAAGGTATAAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAgg                                         <  1:1729754/76‑1 (MQ=255)
                                    aaGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACCAGCCATTAAAATTGCTTTGCGCATGCTGGAACAggg                                        >  1:3582138/1‑76 (MQ=255)
                                    aaGGGCATAAAAGTGGCGGGGAGGAAGGTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAggg                                        <  2:3292955/76‑1 (MQ=255)
                                     aGGGCATCAAATTGGCTGGCCGGGAGGTATGCCTGAACAAGCCATTAAAATTGCTTTGCGCAAGCTGGAACACGGc                                       >  2:5121146/1‑76 (MQ=255)
                                     aGGGCATCAAATGGGCTGGAAGGAAGGTATGCAGAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGc                                       <  1:6212905/76‑1 (MQ=255)
                                     aGGGCATAAAATGGGCTGGAAGAAAGTTATAAATAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGc                                       <  1:9264256/76‑1 (MQ=255)
                                       ggCATAAAGTTGGCGGAGAGGAAGTTATGAATAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCtt                                     <  2:6160472/76‑1 (MQ=255)
                                       ggCATAAAATGGGCTGGAAGGAAGTAATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCtt                                     <  1:7865940/76‑1 (MQ=255)
                                        gCATCAAATGGGCTGGCGGGAGGGTAGACAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCttt                                    <  2:2412591/76‑1 (MQ=255)
                                               aTTGGCTGGGAGGGAGGGATGCATGAACAAACCCTTAAAAATTCTTTGTGCATGCTGCAAAAGGGCCTTGATCGTg                             >  2:4343321/1‑76 (MQ=255)
                                                 tggctggcAGGAAGGTAAACATGCAAAAGACATTAAAAATGCTCTGCGCCTCCAGGAGCACGGCTTTGATCGTGAc                           >  2:893483/1‑76 (MQ=255)
                                                  ggcgggcAGGAAGGTATGCAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGAcc                          <  2:5805333/76‑1 (MQ=255)
                                                   gctggcAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCa                         >  1:5805990/1‑76 (MQ=255)
                                                    ctggcAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAg                        >  1:9219948/1‑76 (MQ=255)
                                                      ggcAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGt                      >  2:110573/1‑76 (MQ=255)
                                                      ggaaGGAAGGTATACATAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGt                      <  2:150509/73‑1 (MQ=255)
                                                       gaaGGAAGGTATGCAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTg                     <  2:8996192/74‑1 (MQ=255)
                                                        cAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGc                    >  1:8275252/1‑76 (MQ=255)
                                                        cAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGc                    >  2:3036758/1‑76 (MQ=255)
                                                        cAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGc                    >  2:8606261/1‑76 (MQ=255)
                                                        cAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGc                    >  1:5356268/1‑76 (MQ=255)
                                                         aggaggGTATACAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   <  2:37117/76‑1 (MQ=255)
                                                         agaaaGGTATGCAGAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   <  2:4760214/73‑1 (MQ=255)
                                                         aagAATGTATGCAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   <  2:5564059/74‑1 (MQ=255)
                                                         aGGAAGTTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   <  2:5115991/76‑1 (MQ=255)
                                                         aGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   >  1:1129134/1‑76 (MQ=255)
                                                         aGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   >  1:3452209/1‑76 (MQ=255)
                                                         aGGAAGGTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   <  2:4982096/76‑1 (MQ=255)
                                                         aGGAAGGTATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   <  2:4329788/76‑1 (MQ=255)
                                                         aGGAAGGTATAAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   <  2:1423069/76‑1 (MQ=255)
                                                         aGGAAGATATGAATAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   <  2:2325413/76‑1 (MQ=255)
                                                         aGGAAGATATGAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCt                   <  2:6217630/76‑1 (MQ=255)
                                                          ggAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTc                  >  1:3247400/1‑76 (MQ=255)
                                                          ggAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTc                  >  1:978474/1‑76 (MQ=255)
                                                           gAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcg                 >  1:930389/1‑76 (MQ=255)
                                                           gAAGGTATAAAAAAAAAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcg                 <  1:3928453/76‑1 (MQ=255)
                                                            aaGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:3554181/1‑76 (MQ=255)
                                                            aaGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:4839655/1‑76 (MQ=255)
                                                            aaGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:1547382/1‑76 (MQ=255)
                                                            aaGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:6253290/1‑76 (MQ=255)
                                                            aaGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:6959536/1‑76 (MQ=255)
                                                            aaGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:7123628/1‑76 (MQ=255)
                                                            aaGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:719172/1‑76 (MQ=255)
                                                            aaGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:1893151/1‑76 (MQ=255)
                                                            aaGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:2672154/1‑76 (MQ=255)
                                                            aaGGGATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgc                >  1:8406009/1‑76 (MQ=255)
                                                             aGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgcg               >  1:1729735/1‑76 (MQ=255)
                                                             aGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTcgcg               >  2:7425822/1‑76 (MQ=255)
                                                                  tGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCAc          >  2:307887/1‑76 (MQ=255)
                                                                     aTGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCa       >  1:3874948/1‑76 (MQ=255)
                                                                     aTGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCa       >  2:5341772/1‑76 (MQ=255)
                                                                     aTGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCa       >  2:9121574/1‑76 (MQ=255)
                                                                      tGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAg      >  1:4723360/1‑76 (MQ=255)
                                                                       gAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGc     >  1:8563810/1‑76 (MQ=255)
                                                                       gAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGc     >  1:8315687/1‑76 (MQ=255)
                                                                       gAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGc     >  1:4097209/1‑76 (MQ=255)
                                                                         aCAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCCGCTa   >  2:9307237/1‑76 (MQ=255)
                                                                          cAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTaa  >  1:7026990/1‑76 (MQ=255)
                                                                          |                                                                           
AGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAA  >  REL606/2304333‑2304482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: