| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,376,348 | 0 | T | G | 44.4% | 242.5 / 70.6 | 187 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| Reads supporting (aligned to +/- strand): ref base T (21/83); new base G (82/1); total (103/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-31 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 268 total aligned reads displayed.
GCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376273‑2376423 | gCACCAGATGAGGGAAACTGTAGACCCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggt < 1:5772574/76‑1 (MQ=255)gCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggt < 1:8766167/76‑1 (MQ=255)gCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggt < 1:6476245/76‑1 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtggt < 2:5025435/76‑1 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtggt < 2:3016762/76‑1 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAgggggt > 2:9312475/1‑76 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAgggggg > 2:3629732/1‑75 (MQ=255) ccAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAgggggg > 2:6789259/1‑75 (MQ=255) aTGAGGCGACCTGTAGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGcca < 2:1730607/76‑2 (MQ=255) aTGAGGCGAACTGTAGACGCCCACTTGGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCt < 2:1368261/76‑1 (MQ=255) tGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCtt > 2:4904229/1‑76 (MQ=255) gAGGCGAACTGTAGACGCCCACTTTGTACCCGGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCttt < 2:8641277/76‑1 (MQ=255) aGGCGAACTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTg < 1:1380438/76‑1 (MQ=255) ggCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGGCCTTGc > 2:2548421/1‑76 (MQ=255) gCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGcc < 1:1771373/76‑1 (MQ=255) cGACCTTTAGCCCCCCACTGTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCa < 1:4359792/76‑1 (MQ=255) cGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCCTTGCCa > 2:4380359/1‑76 (MQ=255) gAACTGTAGACGCCCCCTTTGTGCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCAt < 2:2260077/76‑1 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCGTTGGCAtt > 1:8023956/1‑76 (MQ=255) aaCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCCtt > 1:7295408/1‑76 (MQ=255) aCTGTGGACGCCCCTTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTc < 1:8362307/76‑1 (MQ=255) aCTGTAGCCGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTc < 1:6091364/76‑1 (MQ=255) aCTGTAGACGCCCCCTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTc < 1:8654408/76‑1 (MQ=255) aCTGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTc < 1:1891513/76‑1 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTc < 1:4541792/76‑1 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTc < 1:4571796/76‑1 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGcccttccccttc > 2:3331401/1‑76 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGGCATTc > 2:5089224/1‑76 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCCTTTCCATTc > 2:6129109/1‑76 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTc > 2:1831932/1‑76 (MQ=255) aCTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTc > 2:8147291/1‑76 (MQ=255) tGTAGACGCCCACTTTGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGt < 1:1420923/76‑1 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGt < 1:4491262/76‑1 (MQ=255) tGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGt < 1:2183029/76‑1 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGtc > 1:8511837/1‑75 (MQ=255) gTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCCTTCgca > 1:5389207/1‑74 (MQ=255) tAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGGCTTTGCCATTCGTAc > 1:2996965/1‑76 (MQ=255) gACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGTCCTTTGTAccc > 2:5733934/1‑76 (MQ=255) gACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGGGACCTTTTCATTCGTAccc > 2:6397183/1‑76 (MQ=255) gACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGGCTTTGCCCTTCGTAccc > 1:3361415/1‑76 (MQ=255) gACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCCTTCGTTccc > 1:9232275/1‑76 (MQ=255) cccACTTTGCCCCCCGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCaa < 2:3309798/76‑1 (MQ=255) ccACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCCTTGCCATTCGTACCCGCAAc > 1:1318863/1‑76 (MQ=255) tttGTCCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTa < 1:5500346/76‑1 (MQ=255) tttGTCCCCTGCCCCCATCAGAATCGCCTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTa < 1:749077/76‑1 (MQ=255) tttGTACCCGGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTa < 1:2412376/76‑1 (MQ=255) ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTCCCCGCCACGGGaa > 1:2800445/1‑76 (MQ=255) ttGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGaa > 1:259028/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGGCTTTGCCATTCGTACCCGCCAACGTaaa > 2:1984076/1‑76 (MQ=255) tGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTaaa < 1:9488322/76‑1 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAacac < 1:7127091/76‑1 (MQ=255) aCCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCCCAACGGTAAaaac > 1:7849535/1‑76 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 1:7642561/76‑1 (MQ=255) ccTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGCAAACACaa > 2:8382166/1‑76 (MQ=255) ccTCCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACaa < 1:7996610/76‑1 (MQ=255) cTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCCCAACGGTAAACACaaa > 2:7135023/1‑76 (MQ=255) ttccgccAGCAGAATCGCCTCAAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 2:6675563/74‑1 (MQ=255) tGCCCCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt < 2:3940827/76‑1 (MQ=255) ccgccATCAAAACCGACGCCAGCGAACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATgg < 1:4802888/76‑1 (MQ=255) ccATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGgcgc > 1:211578/1‑76 (MQ=255) ccATCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCCTTGGCCTTCGGACCCGCCACGGGAAAAACAAATGgcgc > 1:5675233/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTg > 1:9381769/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCCTTCGTACCCGCCACGGTAAACACAAAAGGCGCTg > 2:2021718/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAAGGCGCTg > 1:761463/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGGCGCTg > 1:3600354/1‑76 (MQ=255) aTCAGAATCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGGACCCGCAACGGTAAACACAAATGGCGCTg > 2:4618061/1‑76 (MQ=255) aTCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTTcc > 1:6717612/1‑75 (MQ=255) aTCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACCCAAATGGCGCTGGGTTcc > 1:4181015/1‑75 (MQ=255) aTCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTTCa > 1:1039294/1‑76 (MQ=255) tCGACTCCAGTGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAg > 1:8837330/1‑76 (MQ=255) tCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGttttcg > 2:549339/1‑73 (MQ=255) tCGACTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTTCAg > 1:3751227/1‑76 (MQ=255) tCGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTccg > 1:5018202/1‑74 (MQ=255) cGACTCGGGGGTACGCCAGGTGGTTCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 2:1129801/76‑1 (MQ=255) cGACTCCTGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:3308248/76‑1 (MQ=255) cGACTCCAGCGTACGGCAGGTGGTTCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:7729714/76‑1 (MQ=255) cGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAgg < 1:3858302/76‑1 (MQ=255) cGACTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGGTTCAgg > 2:7834755/1‑76 (MQ=255) aCTCCAGCGTACGGCAGGGGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGAc > 2:2374915/1‑76 (MQ=255) aCTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAAGGCGCTGGGTTCAGGAc > 2:5145742/1‑76 (MQ=255) aCTCCAGCGTACGGCAGGGGGGGCCTTTGCCATTCGTACCCGCAACGGGAAACACAAATGGCGCTGGTTTCAGGag > 1:313128/1‑75 (MQ=255) ccAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc < 1:4904229/76‑1 (MQ=255) ccAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGcc < 1:2295845/76‑1 (MQ=255) aGCGTACGGCAGGTTTTCCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCaa < 1:6245834/76‑1 (MQ=255) gCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:5801619/76‑1 (MQ=255) gCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAg < 1:6330786/76‑1 (MQ=255) tACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcg < 1:6933863/76‑1 (MQ=255) tACGGCAGGTGGTCCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGAcg < 2:8004112/76‑1 (MQ=255) ggtggtGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCcgcg > 2:7453130/1‑76 (MQ=255) gtggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa < 2:8023956/76‑1 (MQ=255) gtggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa < 1:1159086/76‑1 (MQ=255) gtggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa < 1:1854722/76‑1 (MQ=255) gtggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa < 2:7295408/76‑1 (MQ=255) gtggtgCCTTTCCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa < 1:6631269/76‑1 (MQ=255) gtggtgCCTTTCCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa < 1:8192739/76‑1 (MQ=255) gtggtgCCTTTCCCATCCGTCCCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGa < 1:52196/76‑1 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 1:4081125/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 2:4535956/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 2:3983703/1‑76 (MQ=255) tggtgCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAc > 2:8171913/1‑76 (MQ=255) | GCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTTCAGGACGCCAAGACGCGCCGCGAC > REL606/2376273‑2376423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |