| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,774,914 | 0 | C | G | 41.0% | 207.3 / 144.5 | 156 | A20P (GCC→CCC) | yicJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (3/89); new base G (64/0); total (67/89) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-40 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 200 total aligned reads displayed.
GGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774840‑3774989 | aGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTggggggg > 2:552907/1‑74 (MQ=255) aaTGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGggggcggcg > 2:4953219/1‑73 (MQ=255) cgaaaaaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCa < 2:1753873/74‑1 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTcccc > 2:4440447/1‑75 (MQ=255) ccAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCa < 2:8517840/76‑1 (MQ=255) cAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCAt < 2:5870556/76‑1 (MQ=255) aaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGCGTCTCCCATGCCa > 1:7040870/1‑76 (MQ=255) aaTATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGGGGCGCCCCTGccg > 2:48497/1‑75 (MQ=255) atatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCACGCCac > 2:4659768/1‑75 (MQ=255) tatCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATa < 1:5240790/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATaa < 1:4734175/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATaa < 2:2738075/76‑1 (MQ=255) atCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATaa < 2:2327729/76‑1 (MQ=255) tCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAAc < 1:501247/76‑1 (MQ=255) tCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAAc < 1:2322120/76‑1 (MQ=255) tCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAAc < 2:3934807/76‑1 (MQ=255) cGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGCGGCTCCCCTCCCCTAAcc > 2:5318518/1‑76 (MQ=255) ggTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCATAACCa > 1:5567198/1‑76 (MQ=255) tataAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAAt < 1:2125866/76‑1 (MQ=255) ataAAAGAACATCATATATAATATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCTCACCaact > 2:2980359/1‑74 (MQ=255) aGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCATAACCAActttttt > 2:1147137/1‑73 (MQ=255) aGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCCTAACCAActttttt > 1:167623/1‑73 (MQ=255) aGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTtctc < 2:6461391/76‑1 (MQ=255) aGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTtctc < 2:3226761/76‑1 (MQ=255) gAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTctct < 1:7119475/76‑1 (MQ=255) gAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTctct < 1:3477341/76‑1 (MQ=255) gAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTctct < 1:3422362/76‑1 (MQ=255) gAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTctct < 1:2236072/76‑1 (MQ=255) aaCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGTCCCCCACCCCCTAAACAAATTTTTCtt > 2:6835137/1‑76 (MQ=255) aCATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCttt < 1:3023827/76‑1 (MQ=255) catcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTa < 1:3930202/76‑1 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGCGGCTCCCATGCCACAACCAATTTTTTCttttt > 2:1505085/1‑74 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTaa < 1:4201131/76‑1 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTaa < 1:1238877/76‑1 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTaa < 1:1517835/76‑1 (MQ=255) atcatATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTaa < 1:4621075/76‑1 (MQ=255) tcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCTAACCAAATTTTTCTTTTAc > 1:519491/1‑76 (MQ=255) tcatATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGCCTCCCCTGCCATAAACAACTTTCTCTTTTAc > 1:354427/1‑76 (MQ=255) catATATAACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCCCCATAACCACATTTTTCTTTTACg > 1:8648076/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCTTCCATAAACAAATTTTTCTTTTACgggc > 1:6778438/1‑74 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCTAACCAATTTTTTTTTTTTCGGac > 1:3287151/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCATAAACAAATTTTTCTTTTACGGac > 1:1975951/1‑76 (MQ=255) atataACATTACGTTATCGAAAATAATGTGGCTGGGGGCGGCCCCCCCGCCATAACCAAATTTCTCTTTAACTGac > 1:2999958/1‑76 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGaca < 1:8081833/76‑1 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGaca < 1:8667486/76‑1 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGaca < 1:3674301/76‑1 (MQ=255) tataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGaca < 1:6693787/76‑1 (MQ=255) ataACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGacaa < 1:920461/76‑1 (MQ=255) aaCATTACGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCACGCCATAACCCAATTTTTCTTTTACCGgcaaca > 2:5088017/1‑76 (MQ=255) aaCATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCCTAACCCACTTTTTCTTTTATCGacaaca > 2:2660558/1‑76 (MQ=255) aaCATTACGTTATCGAAAATAATGTGGCTGGGGGGGCGTCCCGCTCCCTAACCCACTTTCTCTTTTACTGgcaaca > 2:6102375/1‑76 (MQ=255) cATTACGTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCATAACCAAATTTTTCTTTTACAGACCACACt > 2:5666503/1‑76 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACtt < 1:3843271/76‑1 (MQ=255) aTTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACtt < 1:3131960/76‑1 (MQ=255) ttACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTc < 1:5963141/76‑1 (MQ=255) cGTTATCGAAAATAATGTGGCTGGGGGGGGGTCCCCTTCCCTAAACCATTTTTTCTTTTAATGACCAAACTTctct > 2:812149/1‑76 (MQ=255) cGTTATCGAAAATAATGTGGCTGGGGGCGGCTCCCATTCCATTACCAAATTTCTCTTTAACCGACAAAACCTTAct > 2:2184573/1‑76 (MQ=255) gTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCATAACCAAATTTTTCTTTTAATGAACACACATCActc > 1:2705944/1‑76 (MQ=255) gTTATCGAAAATAATGTGGCTGGGGGGGGCTCCCATCCCATAACCAATTTTCTCTTTTAAGGACAACACCTCActt > 1:5456838/1‑75 (MQ=255) ttATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCTCCACAACCAATTTTTTCTTTATCTGGCAACACATTActct > 2:7198963/1‑76 (MQ=255) ttATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCActct < 2:1489859/76‑1 (MQ=255) tATCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCATAACCAAATTTTTCTTTTACGGACAACACTTCACTCtt > 1:7605796/1‑76 (MQ=255) tATCGAAAATAATGTGGCTGGGGGGGGCTCCCCCGCCATAAACACTTTTTTCTTTTACTGACAACACTTCACTCtt > 1:6893364/1‑76 (MQ=255) tATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTCCTCTTTAACGGACAACACTTCACTCtt < 1:4968394/76‑1 (MQ=255) aTCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCATAACCAAATTTTTCTTTTAACGACAAAACCTCACTCttt > 2:1273747/1‑75 (MQ=255) aTCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTc < 2:3840911/76‑1 (MQ=255) aTCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTc < 2:4274924/76‑1 (MQ=255) aTCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTc < 2:8768223/76‑1 (MQ=255) tCGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCATAAACAAATTTCTCTTTTAAGGAGAAAACATCACCCtttt > 2:7506330/1‑74 (MQ=255) tCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCa < 2:9244586/76‑1 (MQ=255) tCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCa < 2:2115073/76‑1 (MQ=255) cGAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCCTAACCCATTTTCTCTTTTACGGGCAACACTTCACTCTTCAt > 2:8239919/1‑76 (MQ=255) gAAAATAATGTGGCTGGGGGGGGCTCCCCTGCCATAACCAAATTTTTTTTTTAAGGACAAAACATCACTCttcttc > 2:9060870/1‑76 (MQ=255) gAAAATAATGTGGCTGGGGGGGGCTCCCATGCCCTAAACAAATTTTTTTTTTACGGCCAACACCTCACCCTTCATc > 2:6616528/1‑76 (MQ=255) aaaaTAATGTGGCTGGGGGGGGCTCCCCTCCCCTAACCCAATTTTTCTTTTACGGACAAAACTTCCCtcttcttct > 2:9428587/1‑76 (MQ=255) aaaTAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTg < 2:1023382/76‑1 (MQ=255) aaaTAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTg < 2:7938738/76‑1 (MQ=255) aaaTAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTg < 2:4378416/76‑1 (MQ=255) aaaTAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTg < 2:9119715/76‑1 (MQ=255) ataatGTGGCTGGGGGGGTCTCCCCCGCCATAACCAAATTTCTCTTTTACGGGTAACACCTTACtcttcttcttct > 2:7091798/1‑76 (MQ=255) ataatGTGGCTGGGGGGGGCTCCCCTGCCCTAACCAATTTTCTCTTTTACCGACAACACTTCACTCTTCCTCTCCt > 2:7724125/1‑76 (MQ=255) aatGTTGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCttt < 2:7305877/76‑1 (MQ=255) atGTTGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt > 2:4248800/1‑76 (MQ=255) atGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 1:7453084/76‑1 (MQ=255) atGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 1:8315258/76‑1 (MQ=255) atGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 1:6089607/76‑1 (MQ=255) atGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 1:5640606/76‑1 (MQ=255) atGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 2:1573797/76‑1 (MQ=255) atGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCtttt < 1:5533/76‑1 (MQ=255) tgtgGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTc < 1:104640/76‑1 (MQ=255) gtgGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTcc < 2:8729866/76‑1 (MQ=255) tggctggcGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCt > 1:9562879/1‑76 (MQ=255) tggctggcGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCt < 2:2510382/76‑1 (MQ=255) ggctggcGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCtt < 2:2004825/76‑1 (MQ=255) gctggcGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTg < 2:3006107/76‑1 (MQ=255) ctggcGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGa > 2:8362600/1‑76 (MQ=255) ctggcGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGa > 1:6616831/1‑76 (MQ=255) tggcGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGAt > 1:1316528/1‑76 (MQ=255) ggcggcGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATg > 2:6585058/1‑76 (MQ=255) ggcggcGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATg < 1:851962/76‑1 (MQ=255) | GGCAGGAATGCCAAAAATATCGGTATAAAAGAACATCATATATAACATTACGTTATCGAAAATAATGTGGCTGGCGGCGTCTCCCATGCCATAACCAATTTTCTCTTTAACGGACAACACTTCACTCTTCATCTGCTTTTCCTTGATGCT > REL606/3774840‑3774989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |