| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,392,556 | 0 | C | T | 40.1% | 164.9 / 128.1 | 187 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (73/39); new base T (0/75); total (73/114) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-23 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 287 total aligned reads displayed.
AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 | cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 1:9280861/76‑1 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa > 1:3704828/1‑76 (MQ=255) cAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTa < 1:3443209/76‑1 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAg > 2:6133416/1‑76 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg < 1:3832282/76‑1 (MQ=255) aaCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAg > 2:3586891/1‑76 (MQ=255) aCTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGc < 2:1322313/76‑1 (MQ=255) cTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGcc < 1:254303/76‑1 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTTGCCa > 1:4859096/1‑76 (MQ=255) ttATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCa < 2:3649378/76‑1 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGGGGTTTTCTAGGCAg > 1:6504279/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGcccg > 1:7407429/1‑74 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGcccg > 1:6043154/1‑74 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAg > 1:931889/1‑76 (MQ=255) tataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAg > 1:7524583/1‑76 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGt > 1:6577721/1‑76 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGAGATTTTCTAGCCAGt > 1:8765961/1‑76 (MQ=255) ataCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGGGATTTTCTAGCCAgc > 1:2819951/1‑75 (MQ=255) cccTGTCTGATGGCGCCCTCGGCGAAGCGGTAACGGGTGAAGGTGCGGGCTGGGATAGCGTTTTTTTAGCCAGTAt < 2:1674451/75‑1 (MQ=255) ccTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGTTGGCGATTTTCTAGCCAGTGtt > 1:4353147/1‑76 (MQ=255) cTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTg > 2:8675811/1‑76 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGGGATTTTCTAGCCAGGATTgg > 1:5284195/1‑76 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGGTTTTCTAGCCAGGATTgg > 1:8600826/1‑76 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTgg > 1:2401904/1‑76 (MQ=255) tGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGGATTgg > 1:408139/1‑76 (MQ=255) gTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCCGTATTGGt > 2:269230/1‑76 (MQ=255) tCTGAGGGCGCGCTCTGGGAAGCGTTAACGCGTGAAGGGGCGGGCGGGGATAGAGTTTTTTTAGCCAGTATTGGTc < 2:4394697/76‑1 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGGAGGGATTTTCTAGCCAGTATTGGTcc > 1:8000979/1‑75 (MQ=255) cTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCCGTATTGGTca > 1:2134678/1‑76 (MQ=255) aTGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTcagca > 2:6733748/1‑76 (MQ=255) tGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTTGTcagcag > 2:7310401/1‑76 (MQ=255) gcgcccTGTGCGATGGGGGAGCGCGTGAGGGTGCTGGTTGGGAAAGATATTTTCTAGCCAGTATTGGTCAGCAttt < 2:7061567/76‑1 (MQ=255) ggcgcTCTGCGAAGCGGTAGCGGGTGAAGGTGCTGGCGGGGATAGCTTTTTTCTAGCCAGTATTGGTCAGCAGTTa < 1:7830099/75‑1 (MQ=255) gcgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAACCAGTATTGGGCAGCAGTTAg > 2:307568/1‑76 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAgg > 1:1745640/1‑76 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGGGATTTTCTAGCCAGTATTGGGCAGCAGTTAgg > 1:359415/1‑76 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAgg > 1:5498751/1‑76 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAgg > 1:9096658/1‑76 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAgg > 1:45363/1‑76 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAgg > 1:7719917/1‑76 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGGCAGCAGTTAgg > 1:2588487/1‑76 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGGATTGGGCAGCAGCTAgg > 1:1195279/1‑76 (MQ=255) cgcTCTGCGAAGCGGTAACGCGTGAAGGGGCTGGCTGGGATAGCGAATTTCTAGCCAGTTTTGGTCAGCAGTTAgg > 1:8955439/1‑76 (MQ=255) cgcTCTGCAAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAgg < 1:5004528/76‑1 (MQ=255) cgcTCGGCGAAGCGTTACCGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAgg < 1:8251760/76‑1 (MQ=255) cgcTCGGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAgg < 1:1434536/76‑1 (MQ=255) cgcTCGGCGAAGCGGTAACGCGTGAAGGTGCTGGCGGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAgg < 1:3255576/76‑1 (MQ=255) gcTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGGTAGCGATTTTCTAGCCAGTATTGGTCAGCCGTTAGGa > 2:7908135/1‑76 (MQ=255) ctctGGGAGGCGGTAACGCGTGAAGGTGCGGGTGGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 2:5228068/76‑1 (MQ=255) ctctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 2:5076609/76‑1 (MQ=255) ctcgGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGaa < 2:4513003/76‑1 (MQ=255) tctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCCGTATTGGTTCGCAGGTAGGAAc > 1:4017339/1‑76 (MQ=255) ctGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTTGTCAGCAGTTAGGAACg > 1:6139670/1‑76 (MQ=255) gCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGGCAGCAGTTAGGAACGGc > 1:4106076/1‑76 (MQ=255) cGAGGCGGTAACGCGTGAAGGTGCTGGGTGGGAGAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCt < 2:328751/76‑1 (MQ=255) cGAGGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGaa < 1:693756/71‑1 (MQ=255) aaGCGGTAACGCGTGAAGGTGCGGGCTGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGa < 2:6388901/76‑1 (MQ=255) aGCGGTACCGCGTGAAGGTGCTGGCTGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGaa < 1:1774744/76‑1 (MQ=255) cgttAACGGGGGAAGCTGGTGGAGGGGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATc < 2:6333368/73‑1 (MQ=255) gTAACGCGTGAGGGTGCGGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATccc < 2:4623816/76‑1 (MQ=255) tAAGGCGTGAAGGTGCGGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCt < 1:6237945/76‑1 (MQ=255) aaCGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGc > 1:7175777/1‑66 (MQ=255) cgcgTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATACCTTGa > 1:4452293/1‑76 (MQ=255) gggTGAAGGTGCGGGTTGGGATAGCATTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa < 1:8508235/74‑1 (MQ=255) gcgTGAAGGTGCTGGCTGGGATAGCTATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGaa < 1:2043026/76‑1 (MQ=255) cgTGAAGGTGCTGGCTGGGGTAGCGATTTTCTTGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAAACCCTTTaaa > 1:1106325/1‑75 (MQ=255) gggTGCGGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgggg < 1:5579832/75‑1 (MQ=255) aGGTGTTGGCTGGGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTgggg < 2:3998965/76‑1 (MQ=255) ggTGTTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggc < 2:563647/76‑1 (MQ=255) gtgtTGGCGGGGATAGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcg < 1:5653226/76‑1 (MQ=255) gTGCTGGCTGGGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcg < 1:10942/76‑1 (MQ=255) gTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCCGTTAGGAACGGCTGAATCCCTTTGACTgggggg > 2:7549294/1‑74 (MQ=255) tGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcgg > 2:1119012/1‑76 (MQ=255) tctggctggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcggc < 2:7191566/75‑1 (MQ=255) gctggctgGGATGGCTTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGggcggc < 1:3895262/76‑1 (MQ=255) gtggcgggGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCt < 1:8307618/75‑1 (MQ=255) ctggctggTAAAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCt < 1:945869/76‑1 (MQ=255) tgggtgggATAGCTTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTg < 1:5908733/76‑1 (MQ=255) tggctggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTg < 1:2242947/76‑1 (MQ=255) gtgtggGATGGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:5460271/73‑1 (MQ=255) gtgtggGATAGCGATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:9028035/73‑1 (MQ=255) ggttggGATGGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:9261446/73‑1 (MQ=255) gggtggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:8362322/73‑1 (MQ=255) gggtggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:2131756/73‑1 (MQ=255) gggtggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:8177312/73‑1 (MQ=255) ggctggGATAGGGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:8239551/76‑1 (MQ=255) ggctggGATAGCGTTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:8286944/76‑1 (MQ=255) ggctggGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:6573302/76‑1 (MQ=255) ggctggGATAGCGTTTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:2398538/76‑1 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg > 1:1912199/1‑76 (MQ=255) ggctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg > 1:2881029/1‑76 (MQ=255) ggcgggGATGGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTgg < 2:3957564/76‑1 (MQ=255) ggtggGATAGCCATTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGc < 2:6828008/74‑1 (MQ=255) gctggGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGc > 1:4957008/1‑76 (MQ=255) gggATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGaa > 2:3806007/1‑76 (MQ=255) gtaTTTTTTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAAt < 1:4248711/74‑1 (MQ=255) gATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGGATGTGAATc > 1:3546179/1‑76 (MQ=255) tttttttAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATcc < 2:403048/75‑1 (MQ=255) tttCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGGATGGGGATcccc > 1:811927/1‑74 (MQ=255) cTAGCCAGTATTGGTCAGCAGTTAGGAACGACTGAATCCCTTGAACTGGGGCGGCTGGCGGATGTGAATCCGCCTg > 1:462951/1‑76 (MQ=255) | AGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCGATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTG > REL606/4392481‑4392631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |