| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,155,456 | C→T | intergenic (+16/‑99) | thrT → / → tuf | tRNA‑Thr/protein chain elongation factor EF‑Tu (duplicate of tufA) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,155,456 | 0 | C | T | 99.6% | 960.7 / ‑4.0 | 239 | intergenic (+16/‑99) | thrT/tuf | tRNA‑Thr/protein chain elongation factor EF‑Tu (duplicate of tufA) |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (148/90); total (148/91) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
Only 100 of 250 total aligned reads displayed.
TAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGAT > REL606/4155384‑4155528 | agagCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGtt > 2:180700/1‑76 (MQ=255) agagCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGtt > 1:2783899/1‑76 (MQ=255) agCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGtttt < 2:4468653/76‑1 (MQ=255) agCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGtttt > 1:6955562/1‑76 (MQ=255) gcgcACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGttttt < 2:8474052/76‑1 (MQ=255) cgcACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGtttttt < 1:5250359/76‑1 (MQ=255) gcACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTc < 2:8160239/76‑1 (MQ=255) gcACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTc < 2:7952120/76‑1 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:3176364/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:432323/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:5156980/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:3866898/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:5839292/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:6048247/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:648849/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:7406998/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:7866568/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:2675239/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:2373404/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:2079265/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:8616140/1‑76 (MQ=255) cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTcc > 2:1985909/1‑76 (MQ=255) ccTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTc < 2:4560512/76‑1 (MQ=255) ttGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTg < 1:769219/76‑1 (MQ=255) ttGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTg < 1:4931897/76‑1 (MQ=255) gTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGttt < 1:287922/76‑1 (MQ=255) tAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTa < 2:1129956/76‑1 (MQ=255) aGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTAtt > 1:7366767/1‑76 (MQ=255) tGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCAt > 1:1235554/1‑76 (MQ=255) tGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCAt > 1:8237274/1‑76 (MQ=255) tGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCAt < 1:8667369/76‑1 (MQ=255) tGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCAt > 1:595374/1‑76 (MQ=255) tGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCAt > 2:9343697/1‑76 (MQ=255) tGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCAt > 1:2297822/1‑76 (MQ=255) tGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCAt > 1:3554569/1‑76 (MQ=255) gAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCAtt < 1:1647508/76‑1 (MQ=255) gAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCAtt < 2:2393548/76‑1 (MQ=255) aGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTc > 2:7567781/1‑76 (MQ=255) ggTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTca < 1:2123563/76‑1 (MQ=255) ggTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTca > 2:37542/1‑76 (MQ=255) ggTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTca < 1:7610757/76‑1 (MQ=255) gTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTcaa > 2:4916975/1‑76 (MQ=255) gTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTcaa < 1:2729433/76‑1 (MQ=255) gTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTcaa < 1:2856593/76‑1 (MQ=255) gTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTcaa < 1:3790794/76‑1 (MQ=255) gTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTcaa < 1:6879595/76‑1 (MQ=255) gTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTcaa > 2:5464779/1‑76 (MQ=255) gTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTcaa < 2:3972553/76‑1 (MQ=255) tCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTcaac < 1:1961212/76‑1 (MQ=255) cAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGt < 1:517106/76‑1 (MQ=255) gTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCg < 2:5639366/76‑1 (MQ=255) gTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCg < 1:961561/76‑1 (MQ=255) gTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCg < 1:1866799/76‑1 (MQ=255) cGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGc < 2:1786532/76‑1 (MQ=255) taTCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAt > 2:6193368/2‑76 (MQ=255) aaTCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAt > 1:3422838/1‑76 (MQ=255) aaTCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAt < 2:6897915/76‑1 (MQ=255) aaTCTGCCTATCAGCACCACTTCTTTTCTCC‑CCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCCTg > 2:7738727/1‑76 (MQ=255) aTCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATg > 2:6801718/1‑76 (MQ=255) aTCTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATg > 1:7358956/1‑76 (MQ=255) cTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGtt > 1:2009891/1‑76 (MQ=255) cTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGtt > 1:7815407/1‑76 (MQ=255) cTGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGtt > 1:1802056/1‑76 (MQ=255) tGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTg > 1:346048/1‑76 (MQ=255) tGCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTg > 1:9195919/1‑76 (MQ=255) gCCTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGc > 1:3378867/1‑76 (MQ=255) ccTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGcc > 1:8060394/1‑76 (MQ=255) cTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCt < 1:8352573/76‑1 (MQ=255) cTATCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCt < 1:4451478/76‑1 (MQ=255) aTCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTgg > 1:1508303/1‑76 (MQ=255) aTCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTgg < 1:1116656/76‑1 (MQ=255) tCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGt < 2:9026538/76‑1 (MQ=255) tCAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGt < 2:5336941/76‑1 (MQ=255) cAGCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGtt > 1:6355368/1‑76 (MQ=255) tgCACCACTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTg > 2:9365772/2‑76 (MQ=255) ccacTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGAtgt > 2:535962/1‑76 (MQ=255) ccacTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGAtgt > 2:8652499/1‑76 (MQ=255) ccacTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGAtgt > 1:3930630/1‑76 (MQ=255) ccacTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGAtgt > 2:8391353/1‑76 (MQ=255) acTTCTTTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATgtgg > 2:3715632/1‑76 (MQ=255) cttcttTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATgtggt > 2:6319416/1‑76 (MQ=255) ttcttTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATgtggtg < 1:9067614/76‑1 (MQ=255) ttcttTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATgtggtg < 2:9075937/76‑1 (MQ=255) ttcttTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATgtggtg < 2:4707788/76‑1 (MQ=255) ttcttTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATgtggtg < 1:5897155/76‑1 (MQ=255) tcttTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGa > 2:6207058/1‑76 (MQ=255) tcttTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGa < 1:3203277/76‑1 (MQ=255) cttTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGat < 1:5596771/76‑1 (MQ=255) cttTTCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGat > 1:5712463/1‑76 (MQ=255) tttCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAGGTCGGGCATGTTGCCTGGTTGATGTGGTGatat > 1:4264778/1‑76 (MQ=255) tttCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGatat < 1:3296680/76‑1 (MQ=255) tttCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGatat < 1:3172411/76‑1 (MQ=255) tttCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGatat < 1:4784731/76‑1 (MQ=255) ttCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATc < 1:6615325/76‑1 (MQ=255) ttCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATc > 2:6598042/1‑76 (MQ=255) ttCTCCTCCTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATc < 1:7878272/76‑1 (MQ=255) tcctccTTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCAcc > 2:3445888/1‑76 (MQ=255) cctcctTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCg > 2:2811048/1‑76 (MQ=255) cctcctTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCg > 2:5980219/1‑76 (MQ=255) cctcctTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCg > 1:3737695/1‑76 (MQ=255) | TAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGAT > REL606/4155384‑4155528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |