| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,593,944 | T→A | I166N (ATC→AAC) | bglJ → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,593,944 | 0 | T | A | 99.1% | 1319.8 / 0.7 | 331 | I166N (ATC→AAC) | bglJ | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base A (125/203); total (127/204) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01 | |||||||||||
Only 100 of 332 total aligned reads displayed.
ATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGA > REL606/4593871‑4594016 | atatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCTGAGCAGCTTAAACGCAATATCAAAACGAAcc < 2:9219534/76‑1 (MQ=255)atatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAAcc < 2:1150030/76‑1 (MQ=255)atatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAAcc < 2:669880/76‑1 (MQ=255) tatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCg < 1:821825/76‑1 (MQ=255) tatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCg > 1:5703544/1‑76 (MQ=255) tatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCg < 1:3254307/76‑1 (MQ=255) agatGCGATGTATGGCGCGTGGCTACTCAATGACACAAATGGGCGAGCAGCTTAAACGCAATATCAAAACGAACCg < 1:645240/73‑1 (MQ=255) cTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACAt < 2:4356327/76‑1 (MQ=255) cTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACAt < 2:6379741/76‑1 (MQ=255) ttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATaa < 1:1439291/76‑1 (MQ=255) ttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATaa > 2:4411099/1‑76 (MQ=255) ttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATaa > 1:7508944/1‑76 (MQ=255) tATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATaaa > 2:5770956/1‑76 (MQ=255) tGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAAtt < 1:4655226/76‑1 (MQ=255) tCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTa > 2:7586098/1‑76 (MQ=255) tCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTa < 2:7213677/76‑1 (MQ=255) tCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTa > 2:7080972/1‑76 (MQ=255) tGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAAtgtg < 1:9542672/76‑1 (MQ=255) tGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAAtgtg < 1:7950226/76‑1 (MQ=255) gCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGAt > 1:1200388/1‑76 (MQ=255) cTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATg > 2:7503255/1‑76 (MQ=255) cTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATg > 1:1746600/1‑76 (MQ=255) tACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGt < 2:2806073/76‑1 (MQ=255) cTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCg < 1:799853/76‑1 (MQ=255) cTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCg < 1:7047463/76‑1 (MQ=255) cAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaa > 1:2795163/1‑76 (MQ=255) cAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaa > 1:5374506/1‑76 (MQ=255) cAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaa < 2:3333294/76‑1 (MQ=255) cAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaa > 1:3458720/1‑76 (MQ=255) aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGaaa < 2:5974131/76‑1 (MQ=255) tGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACt > 1:5551687/1‑76 (MQ=255) gACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTg < 1:7724954/76‑1 (MQ=255) gACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTg < 1:2491936/76‑1 (MQ=255) gACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTg > 1:8257846/1‑76 (MQ=255) aaaTTGCAGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGt < 2:4273593/76‑1 (MQ=255) aaTTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTc > 2:4806852/1‑76 (MQ=255) aaTTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTc < 2:7898865/76‑1 (MQ=255) ttGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg < 2:1746600/76‑1 (MQ=255) ttGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg < 2:6554447/76‑1 (MQ=255) ttGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg > 1:3586476/1‑76 (MQ=255) ttGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg < 2:8305007/76‑1 (MQ=255) tGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGt > 1:1521204/1‑76 (MQ=255) gCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGtt < 2:2463953/76‑1 (MQ=255) cGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCt > 2:9447195/1‑76 (MQ=255) cGAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCt > 1:653981/1‑76 (MQ=255) gAGCAGCTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTg < 2:5670342/76‑1 (MQ=255) agcagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGa < 2:5551687/76‑1 (MQ=255) agcagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGa < 2:1908296/76‑1 (MQ=255) cagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACg > 2:4949110/1‑76 (MQ=255) cagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACg > 2:31861/1‑76 (MQ=255) cagcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACg > 1:2200326/1‑76 (MQ=255) agcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGc > 2:2334/1‑76 (MQ=255) agcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGc > 2:4622935/1‑76 (MQ=255) agcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGc > 2:2644454/1‑76 (MQ=255) gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:2887848/76‑1 (MQ=255) gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:3544233/76‑1 (MQ=255) gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:3659341/76‑1 (MQ=255) gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:421483/76‑1 (MQ=255) gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:8398168/76‑1 (MQ=255) gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 1:6753474/76‑1 (MQ=255) gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:1970110/76‑1 (MQ=255) gcTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 2:4560281/76‑1 (MQ=255) cTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAg < 1:3900820/76‑1 (MQ=255) cTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAg < 2:7348455/76‑1 (MQ=255) cTTAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAg < 1:5525208/76‑1 (MQ=255) tAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg < 2:7042142/76‑1 (MQ=255) tAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg < 2:8632926/76‑1 (MQ=255) tAAACGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg < 2:5255243/76‑1 (MQ=255) aaaCGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgt < 2:8770220/76‑1 (MQ=255) aaaCGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgt > 1:5924425/1‑76 (MQ=255) aCGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttg < 2:3475637/76‑1 (MQ=255) aCGCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttg < 2:6228397/76‑1 (MQ=255) gCAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgtt < 2:4686128/76‑1 (MQ=255) cAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg > 2:51623/1‑76 (MQ=255) cAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg < 2:8043714/76‑1 (MQ=255) cAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg < 2:9060441/76‑1 (MQ=255) cAATATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg < 2:2952190/76‑1 (MQ=255) aaTATCAAAACGACCTGTGCCCATAGATTTCATGTGAGGTCGAAACCCGGCCTCAGTTCTGGCGGAGGGTTGTTgg < 2:1414446/76‑1 (MQ=255) aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 2:2683532/76‑1 (MQ=255) aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 2:8462993/76‑1 (MQ=255) aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 2:5070660/76‑1 (MQ=255) aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 2:1559067/76‑1 (MQ=255) aaTATCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTgg < 1:4158874/76‑1 (MQ=255) tatCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAg > 2:2301817/1‑76 (MQ=255) tCAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGc < 2:8897656/76‑1 (MQ=255) taaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGcc > 1:1420528/2‑76 (MQ=255) cAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGcc > 1:2907519/1‑76 (MQ=255) cAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGcc > 1:7316145/1‑76 (MQ=255) cAAAACGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGcc > 1:8400483/1‑76 (MQ=255) aaaaCGAACCGTGCACATAAATTTAATGTGATGTCTAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg < 2:3881734/76‑1 (MQ=255) aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg < 2:3415701/76‑1 (MQ=255) aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg > 2:692727/1‑76 (MQ=255) aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg > 2:1444056/1‑76 (MQ=255) aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg > 2:2358861/1‑76 (MQ=255) aaaaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg < 2:4450523/76‑1 (MQ=255) aaCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCa < 1:192540/76‑1 (MQ=255) aCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg > 1:4431954/1‑76 (MQ=255) aCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg > 1:3738735/1‑76 (MQ=255) aCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg < 2:5875355/76‑1 (MQ=255) aCGAACCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAg > 1:908379/1‑76 (MQ=255) | ATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGA > REL606/4593871‑4594016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |